ID   AZG1_PAELB              Reviewed;         441 AA.
AC   A0A2A5K5H4;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Nucleobase transporter PlAzg1 {ECO:0000303|PubMed:29385571};
GN   ORFNames=PL1_1235 {ECO:0000312|EMBL:PCK72294.1};
OS   Paenibacillus larvae subsp. larvae (strain NRRL B-3650 / LMG 16245).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=741161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=21923906; DOI=10.1186/1471-2164-12-450;
RA   Chan Q.W., Cornman R.S., Birol I., Liao N.Y., Chan S.K., Docking T.R.,
RA   Jackman S.D., Taylor G.A., Jones S.J., de Graaf D.C., Evans J.D.,
RA   Foster L.J.;
RT   "Updated genome assembly and annotation of Paenibacillus larvae, the agent
RT   of American foulbrood disease of honey bees.";
RL   BMC Genomics 12:450-450(2011).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=29385571; DOI=10.1093/femsle/fny018;
RA   Alexander C.R., Dingman D.W., Schultes N.P., Mourad G.S.;
RT   "The solute transport profile of two Aza-guanine transporters from the
RT   Honey bee pathogen Paenibacillus larvae.";
RL   FEMS Microbiol. Lett. 365:0-0(2018).
CC   -!- FUNCTION: Transports adenine, hypoxanthine, xanthine and uracil.
CC       Transport is probably proton-dependent. {ECO:0000269|PubMed:29385571}.
CC   -!- ACTIVITY REGULATION: Inhibited by the proton gradient disruptor
CC       carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by the sodium
CC       gradient disruptor ouabain. {ECO:0000269|PubMed:29385571}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=2.95 uM for adenine {ECO:0000269|PubMed:29385571};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. Azg-like subfamily. {ECO:0000305}.
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DR   EMBL; ADZY03000026; PCK72294.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A5K5H4; -.
DR   SMR; A0A2A5K5H4; -.
DR   EnsemblBacteria; PCK72294; PCK72294; PL1_1235.
DR   Proteomes; UP000217691; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:1904823; P:purine nucleobase transmembrane transport; IEA:UniProt.
DR   InterPro; IPR045018; Azg-like.
DR   InterPro; IPR026033; Azg-like_bact_archaea.
DR   InterPro; IPR006043; NCS2.
DR   PANTHER; PTHR43337; PTHR43337; 1.
DR   Pfam; PF00860; Xan_ur_permease; 1.
DR   PIRSF; PIRSF005353; PbuG; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..441
FT                   /note="Nucleobase transporter PlAzg1"
FT                   /id="PRO_0000446239"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        347..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        421..441
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   441 AA;  47611 MW;  4C0FA09D7E1A413E CRC64;
     MKGTFDVSRW ETWFQLKERG TTWTTEILAG CTTFMTMVFI LVVNPAILSD AGMDFNGVYV
     ATVLVTLIST LIIALFGNFP FVIAPGMGIN AFFAYSVVKA QGIPWQTALG SVFLAGTVFL
     VLALTRYRRF LLDAIPQSLK YAITAGTGLF ICFVGLQNAK LVISSPDTLV TLGNLREPGT
     LLSIIGLAVT LLLMTYRIRG ALFLGMIVTS VLAWIMGLMQ LPSHFLSIPS GLAHTALQLD
     IDGVFNYDML AVTFTFLLIS VFETTGTMVS LAEQAGLMKE GRFPHSRSAL LANAVGVTSG
     ALLGTSPITT LVESGSGIAA GGRTGLTPIV TCILLVITMF FAPVAETLAS TPFVTAPALV
     IVGFSMLEEM VHVEWKSFEE AFPSFLVMVT MPLTYSVSSA IGIGFIVYVL LKLFRGKAKE
     VHPALYFFAL FFFIQLGFIH S