ID   AZG2_PAELB              Reviewed;         498 AA.
AC   A0A2A5K485;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Nucleobase transporter PlAzg2 {ECO:0000303|PubMed:29385571};
GN   ORFNames=PL1_1481 {ECO:0000312|EMBL:PCK71842.1};
OS   Paenibacillus larvae subsp. larvae (strain NRRL B-3650 / LMG 16245).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=741161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=21923906; DOI=10.1186/1471-2164-12-450;
RA   Chan Q.W., Cornman R.S., Birol I., Liao N.Y., Chan S.K., Docking T.R.,
RA   Jackman S.D., Taylor G.A., Jones S.J., de Graaf D.C., Evans J.D.,
RA   Foster L.J.;
RT   "Updated genome assembly and annotation of Paenibacillus larvae, the agent
RT   of American foulbrood disease of honey bees.";
RL   BMC Genomics 12:450-450(2011).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=29385571; DOI=10.1093/femsle/fny018;
RA   Alexander C.R., Dingman D.W., Schultes N.P., Mourad G.S.;
RT   "The solute transport profile of two Aza-guanine transporters from the
RT   Honey bee pathogen Paenibacillus larvae.";
RL   FEMS Microbiol. Lett. 365:0-0(2018).
CC   -!- FUNCTION: Transports adenine, guanine, hypoxanthine, xanthine, cytosine
CC       and uracil. Transport is probably proton-dependent.
CC       {ECO:0000269|PubMed:29385571}.
CC   -!- ACTIVITY REGULATION: Inhibited by the proton gradient disruptor
CC       carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by the sodium
CC       gradient disruptor ouabain. {ECO:0000269|PubMed:29385571}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=1.92 uM for adenine {ECO:0000269|PubMed:29385571};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. Azg-like subfamily. {ECO:0000305}.
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DR   EMBL; ADZY03000057; PCK71842.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A5K485; -.
DR   SMR; A0A2A5K485; -.
DR   EnsemblBacteria; PCK71842; PCK71842; PL1_1481.
DR   Proteomes; UP000217691; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:1904823; P:purine nucleobase transmembrane transport; IEA:UniProt.
DR   InterPro; IPR045018; Azg-like.
DR   InterPro; IPR006043; NCS2.
DR   PANTHER; PTHR43337; PTHR43337; 1.
DR   Pfam; PF00860; Xan_ur_permease; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..498
FT                   /note="Nucleobase transporter PlAzg2"
FT                   /id="PRO_0000446240"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        478..498
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   498 AA;  53246 MW;  6A67A8B63C15A55A CRC64;
     MYVSSLLFLY ASESYESFIF HFQMCYNKAH NGFMPSIFQQ LKGLGEGLQW KVGISLDHQE
     KQTSSHEKEM TSPNSATRLI PSDWRRELLA GTVSFFAAVY IIIVNSSILA DAGIPQEAGI
     IATILASAIG CFIMGLWGNA PLVIVPGMGI NAMFTYTLVQ GMGLTWQQAL AAVMMSGICF
     FAISMTSLVE KLRTAIPASL QEAISVGIGL MLVLIGLHKG GVIASDRSSV IAVQSFADPG
     VLVTLATLAL TCILYIRKVP GNLLLAIIGG SALAYLFKAV PSKAATGVGG SSWSSYGDLF
     GQLSVKGASI TTLVIAVFSL TLVIVFENVG LINAQLKMSG RTERFKRVTQ ATSLTVILSG
     IFGTSPTVST VEAAAGISAG GRTGWASIAT GTLFLLSFIA MPVITLVPDQ AVAPILIFIG
     GLMMPAVRHI SFERMEEGLP AFFIIAFIPL MHSIVDGIAI GFISYALFHI AVGKWREVKP
     LFYIISLLFV MHFVLQTM