ID   AZI2_MACFA              Reviewed;         394 AA.
AC   Q4R3X1; Q4R7X6;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=5-azacytidine-induced protein 2;
GN   Name=AZI2; ORFNames=QtsA-13627, QtsA-14164;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Adapter protein which binds TBK1 and IKBKE playing a role in
CC       antiviral innate immunity (By similarity). Activates serine/threonine-
CC       protein kinase TBK1 and facilitates its oligomerization (By
CC       similarity). Enhances the phosphorylation of NF-kappa-B p65 subunit
CC       RELA by TBK1 (By similarity). Promotes TBK1-induced as well as TNF-
CC       alpha or PMA-induced activation of NF-kappa-B (By similarity).
CC       Participates in IFNB promoter activation via TICAM1 (By similarity).
CC       {ECO:0000250|UniProtKB:Q9H6S1}.
CC   -!- SUBUNIT: Homodimer (By similarity). Interacts with IKBKE, TBK1 and
CC       TICAM1 (By similarity). {ECO:0000250|UniProtKB:Q9H6S1,
CC       ECO:0000250|UniProtKB:Q9QYP6}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H6S1}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q4R3X1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q4R3X1-2; Sequence=VSP_023821;
CC   -!- PTM: Ubiquitinated via 'Lys-48'-linked polyubiquitination by TRIM38,
CC       leading to its degradation. {ECO:0000250|UniProtKB:Q9QYP6}.
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DR   EMBL; AB168685; BAE00796.1; -; mRNA.
DR   EMBL; AB179144; BAE02195.1; -; mRNA.
DR   AlphaFoldDB; Q4R3X1; -.
DR   SMR; Q4R3X1; -.
DR   STRING; 9541.XP_005545633.1; -.
DR   eggNOG; ENOG502QV07; Eukaryota.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR024581; TBD.
DR   Pfam; PF12845; TBD; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Phosphoprotein;
KW   Reference proteome; Ubl conjugation.
FT   CHAIN           1..394
FT                   /note="5-azacytidine-induced protein 2"
FT                   /id="PRO_0000280603"
FT   REGION          1..197
FT                   /note="Homodimerization"
FT                   /evidence="ECO:0000250"
FT   REGION          216..257
FT                   /note="Interaction with TBK1 and IKBKE"
FT                   /evidence="ECO:0000250"
FT   REGION          355..379
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          40..76
FT                   /evidence="ECO:0000255"
FT   COILED          102..196
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        355..369
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         318
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q4KMA0"
FT   MOD_RES         355
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6S1"
FT   VAR_SEQ         1..51
FT                   /note="MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKR
FT                   -> MNLLLPILLLSLHMKTSKND (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_023821"
FT   CONFLICT        191
FT                   /note="R -> Q (in Ref. 1; BAE00796)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   394 AA;  45053 MW;  000182BB41886B4F CRC64;
     MDALVEDDIC ILNHEKAHKR DTVTPVSIYS GDESVASHFA LVTAYEDIKK RLKDSEKENS
     LLKKRIRFLE EKLIARFDEE TSSVGREQVN KAYHAYREVC IDRDNLKSKL DKMNKDNSES
     LKVLNEQLQS KEVELLQLRT EVETQQVMRN LNTPSSNWEV EKLSCDLKIH GLEQELELMR
     KECSDLKIEL RKAKQTDPYQ EDNLKSRDLQ KLSISSDNMQ HAYWELKREM SNLHLVTQVQ
     AELLRKLKTS TAIKKACAPV GCSEDLGRDS TKLHLMNFTA TYTRHPPLSP NGKALCHAAS
     SPLPGDIKVL SEKAVLQSWT DNERSIPNDG TCFQEHSSYG RNSLSLEDNS WVFPSPPKSS
     ETAFGETKSK TLPLPNLPPL HYLDQHNQNC LYKN