ID   AZO1_STAAM              Reviewed;         188 AA.
AC   Q99W49;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=FMN-dependent NADPH-azoreductase;
DE            EC=1.7.-.-;
DE   AltName: Full=NADPH-dependent flavo-azoreductase;
DE   AltName: Full=NADPH-flavin azoreductase;
GN   Name=azo1; OrderedLocusNames=SAV0560;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo
CC       compounds to the corresponding amines. Requires NADPH, but not NADH, as
CC       an electron donor for its activity (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the azoreductase type 2 family. {ECO:0000305}.
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DR   EMBL; BA000017; BAB56722.1; -; Genomic_DNA.
DR   RefSeq; WP_000677261.1; NC_002758.2.
DR   AlphaFoldDB; Q99W49; -.
DR   SMR; Q99W49; -.
DR   PaxDb; Q99W49; -.
DR   EnsemblBacteria; BAB56722; BAB56722; SAV0560.
DR   KEGG; sav:SAV0560; -.
DR   HOGENOM; CLU_055322_1_2_9; -.
DR   OMA; PEYHSGM; -.
DR   PhylomeDB; Q99W49; -.
DR   BioCyc; SAUR158878:SAV_RS03125-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NADP; Oxidoreductase.
FT   CHAIN           1..188
FT                   /note="FMN-dependent NADPH-azoreductase"
FT                   /id="PRO_0000245992"
SQ   SEQUENCE   188 AA;  20912 MW;  D7CA1764A344D641 CRC64;
     MKGLIIIGSA QVNSHTSALA RYLTEHFKTH DIEAEIFDLA EKPLNQLDFS GTTPSIDEIK
     QNMKDLKEKA MAADFLILGT PNYHGSYSGI LKNALDHLNM DYFKMKPVGL IGNSGGIVSS
     EPLSHLRVIV RSLLGIAVPT QIATHDSDFA KNEDGSYYLN DSEFQLRARL FVDQIVSFVN
     NSPYEHLK