ID   AZO1_STAEQ              Reviewed;         188 AA.
AC   Q5HRI7;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=FMN-dependent NADPH-azoreductase;
DE            EC=1.7.-.-;
DE   AltName: Full=NADPH-dependent flavo-azoreductase;
DE   AltName: Full=NADPH-flavin azoreductase;
GN   Name=azo1; OrderedLocusNames=SERP0206;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo
CC       compounds to the corresponding amines. Requires NADPH, but not NADH, as
CC       an electron donor for its activity (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the azoreductase type 2 family. {ECO:0000305}.
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DR   EMBL; CP000029; AAW53570.1; -; Genomic_DNA.
DR   RefSeq; WP_002438713.1; NC_002976.3.
DR   AlphaFoldDB; Q5HRI7; -.
DR   SMR; Q5HRI7; -.
DR   STRING; 176279.SERP0206; -.
DR   EnsemblBacteria; AAW53570; AAW53570; SERP0206.
DR   KEGG; ser:SERP0206; -.
DR   eggNOG; COG0431; Bacteria.
DR   HOGENOM; CLU_055322_1_2_9; -.
DR   OMA; PEYHSGM; -.
DR   OrthoDB; 1818437at2; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..188
FT                   /note="FMN-dependent NADPH-azoreductase"
FT                   /id="PRO_0000245997"
SQ   SEQUENCE   188 AA;  20687 MW;  32B05315CC80E2EE CRC64;
     MKGLIIIGSA QVGSHTNALS KYLKGQLGEH DVEVEIFDLA EKPIHQLDFA GTTQAVDEIK
     NNVKSLQSKA MEADFLILGT PNYHGSFSGI LKNALDHLNM DHFKMKPVGL ICNSGGIVSS
     EPLSHLRVIV RSLLGIAVPT QIATHDSDYA KLEDGTLYLE DNEFQLRAKL FVDQIVSFVT
     NSPYEHLK