ID   AZO1_STAES              Reviewed;         188 AA.
AC   Q8CTR9;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=FMN-dependent NADPH-azoreductase;
DE            EC=1.7.-.-;
DE   AltName: Full=NADPH-dependent flavo-azoreductase;
DE   AltName: Full=NADPH-flavin azoreductase;
GN   Name=azo1; OrderedLocusNames=SE_0330;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo
CC       compounds to the corresponding amines. Requires NADPH, but not NADH, as
CC       an electron donor for its activity (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the azoreductase type 2 family. {ECO:0000305}.
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DR   EMBL; AE015929; AAO03927.1; -; Genomic_DNA.
DR   RefSeq; NP_763885.1; NC_004461.1.
DR   RefSeq; WP_001832301.1; NZ_WBME01000014.1.
DR   AlphaFoldDB; Q8CTR9; -.
DR   SMR; Q8CTR9; -.
DR   STRING; 176280.SE_0330; -.
DR   EnsemblBacteria; AAO03927; AAO03927; SE_0330.
DR   GeneID; 50019506; -.
DR   KEGG; sep:SE_0330; -.
DR   PATRIC; fig|176280.10.peg.304; -.
DR   eggNOG; COG0431; Bacteria.
DR   HOGENOM; CLU_055322_1_2_9; -.
DR   OMA; PEYHSGM; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NADP; Oxidoreductase.
FT   CHAIN           1..188
FT                   /note="FMN-dependent NADPH-azoreductase"
FT                   /id="PRO_0000245998"
SQ   SEQUENCE   188 AA;  20730 MW;  3E404E15D2811FEE CRC64;
     MKGLIIIGSA QVGSHTNALS KYLKGQLGEH DVEVEIFDLA EKPIHQLDFA GTTQAVDEIK
     NNVKSLQNKA MEADFLILGT PNYHGSFSGI LKNALDHLNM DHFKMKPVGL ICNSGGIVSS
     EPLSHLRVIV RSLLGIAVPT QIATHDSDYA KLEDGTLYLE DNEFQLRSKL FVDQIVSFVT
     NSPYEHLK