ID   AZO1_STAHJ              Reviewed;         188 AA.
AC   Q4L3N6;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=FMN-dependent NADPH-azoreductase;
DE            EC=1.7.-.-;
DE   AltName: Full=NADPH-dependent flavo-azoreductase;
DE   AltName: Full=NADPH-flavin azoreductase;
GN   Name=azo1; OrderedLocusNames=SH2432;
OS   Staphylococcus haemolyticus (strain JCSC1435).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=279808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCSC1435;
RX   PubMed=16237012; DOI=10.1128/jb.187.21.7292-7308.2005;
RA   Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y.,
RA   Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C.,
RA   Hiramatsu K.;
RT   "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the
RT   extreme plasticity of its genome and the evolution of human-colonizing
RT   staphylococcal species.";
RL   J. Bacteriol. 187:7292-7308(2005).
CC   -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo
CC       compounds to the corresponding amines. Requires NADPH, but not NADH, as
CC       an electron donor for its activity (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the azoreductase type 2 family. {ECO:0000305}.
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DR   EMBL; AP006716; BAE05741.1; -; Genomic_DNA.
DR   RefSeq; WP_011276686.1; NC_007168.1.
DR   AlphaFoldDB; Q4L3N6; -.
DR   SMR; Q4L3N6; -.
DR   STRING; 279808.SH2432; -.
DR   EnsemblBacteria; BAE05741; BAE05741; SH2432.
DR   KEGG; sha:SH2432; -.
DR   eggNOG; COG0431; Bacteria.
DR   HOGENOM; CLU_055322_1_2_9; -.
DR   OMA; PEYHSGM; -.
DR   OrthoDB; 1818437at2; -.
DR   Proteomes; UP000000543; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NADP; Oxidoreductase.
FT   CHAIN           1..188
FT                   /note="FMN-dependent NADPH-azoreductase"
FT                   /id="PRO_0000245999"
SQ   SEQUENCE   188 AA;  20724 MW;  847223EB21D5F979 CRC64;
     MKGLIIVGSA KVGSHTNALA KYLVGQFDTH DLDVDIYDLA ERPLNQLDFS GTTPSIDEIK
     TNIKDFQEKV MAADFLVLGT PNYHGSYSGI LKNALDHINM DYVKMKPVGL IGNSGGIVSS
     EPLSHLRVIV RSLLGIAVPT QIATHDSDYS KLDDGTLYLD DDQFQLRAKL FVDQIVSFVN
     NSPYEHLK