ID   AZO1_STAS1              Reviewed;         188 AA.
AC   Q49VB1;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=FMN-dependent NADPH-azoreductase;
DE            EC=1.7.-.-;
DE   AltName: Full=NADPH-dependent flavo-azoreductase;
DE   AltName: Full=NADPH-flavin azoreductase;
GN   Name=azo1; OrderedLocusNames=SSP2154;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Catalyzes the reductive cleavage of azo bond in aromatic azo
CC       compounds to the corresponding amines. Requires NADPH, but not NADH, as
CC       an electron donor for its activity (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the azoreductase type 2 family. {ECO:0000305}.
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DR   EMBL; AP008934; BAE19299.1; -; Genomic_DNA.
DR   RefSeq; WP_011303787.1; NZ_MTGA01000039.1.
DR   AlphaFoldDB; Q49VB1; -.
DR   SMR; Q49VB1; -.
DR   STRING; 342451.SSP2154; -.
DR   EnsemblBacteria; BAE19299; BAE19299; SSP2154.
DR   KEGG; ssp:SSP2154; -.
DR   PATRIC; fig|342451.11.peg.2145; -.
DR   eggNOG; COG0431; Bacteria.
DR   HOGENOM; CLU_055322_1_2_9; -.
DR   OMA; PEYHSGM; -.
DR   OrthoDB; 1818437at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.360; -; 1.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR005025; FMN_Rdtase-like.
DR   Pfam; PF03358; FMN_red; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..188
FT                   /note="FMN-dependent NADPH-azoreductase"
FT                   /id="PRO_0000246000"
SQ   SEQUENCE   188 AA;  20909 MW;  B69FD2DC2ADA05E8 CRC64;
     MKGLIIVGSA KVGSHTTALS QYLKGHFEEH DFDVEIFDLA EQPIHQLDVS GASNLSESYK
     NNLQTLKTKA READFFVLGS PNYHGSYSGI LKNALDHLTM DDFKMKPVGL IGNSGGIVSS
     EPLSHLRLIV RSLLGIAVPT QIATHDTDFK KLDDGTYYLA NEEFQLRARL FVDQIISFVK
     NSPYEHLK