ID   ABA3_BOTFU              Reviewed;         417 AA.
AC   Q14RS2;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Alpha-ionylideneethane synthase aba3 {ECO:0000303|PubMed:30226766};
DE            EC=4.2.3.- {ECO:0000269|PubMed:30226766};
DE   AltName: Full=Abscisic acid biosynthesis cluster protein 3 {ECO:0000303|PubMed:30226766};
DE   AltName: Full=Sesquiterpene synthase aba3 {ECO:0000303|PubMed:30226766};
GN   Name=aba3 {ECO:0000303|PubMed:16820452};
OS   Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Sclerotiniaceae; Botrytis.
OX   NCBI_TaxID=40559;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, DISRUPTION
RP   PHENOTYPE, AND PATHWAY.
RC   STRAIN=SAS56;
RX   PubMed=16820452; DOI=10.1128/aem.02919-05;
RA   Siewers V., Kokkelink L., Smedsgaard J., Tudzynski P.;
RT   "Identification of an abscisic acid gene cluster in the grey mold Botrytis
RT   cinerea.";
RL   Appl. Environ. Microbiol. 72:4619-4626(2006).
RN   [2]
RP   FUNCTION.
RX   PubMed=15240257; DOI=10.1128/aem.70.7.3868-3876.2004;
RA   Siewers V., Smedsgaard J., Tudzynski P.;
RT   "The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis
RT   in Botrytis cinerea.";
RL   Appl. Environ. Microbiol. 70:3868-3876(2004).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=30226766; DOI=10.1021/jacs.8b08925;
RA   Takino J., Kozaki T., Sato Y., Liu C., Ozaki T., Minami A., Oikawa H.;
RT   "Unveiling biosynthesis of the phytohormone abscisic acid in fungi:
RT   unprecedented mechanism of core scaffold formation catalyzed by an unusual
RT   sesquiterpene synthase.";
RL   J. Am. Chem. Soc. 140:12392-12395(2018).
CC   -!- FUNCTION: Alpha-ionylideneethane synthase; part of the gene cluster
CC       that mediates the biosynthesis of abscisic acid (ABA), a phytohormone
CC       that acts antagonistically toward salicylic acid (SA), jasmonic acid
CC       (JA) and ethylene (ETH) signaling, to impede plant defense responses
CC       (PubMed:15240257, PubMed:16820452). The first step of the pathway
CC       catalyzes the reaction from farnesyl diphosphate to alpha-
CC       ionylideneethane performed by the alpha-ionylideneethane synthase aba3
CC       via a three-step reaction mechanism involving 2 neutral intermediates,
CC       beta-farnesene and allofarnesene (PubMed:30226766). The cytochrome P450
CC       monooxygenase aba1 might then be involved in the conversion of alpha-
CC       ionylideneethane to alpha-ionylideneacetic acid (Probable). Alpha-
CC       ionylideneacetic acid is further converted to abscisic acid in 2 steps
CC       involving the cytochrome P450 monooxygenase aba2 and the short-chain
CC       dehydrogenase/reductase aba4, via the intermediates 1'-deoxy-ABA or
CC       1',4'-trans-diol-ABA, depending on the order of action of these 2
CC       enzymes (Probable). Aba2 is responsible for the hydroxylation of carbon
CC       atom C-1' and aba4 might be involved in the oxidation of the C-4'
CC       carbon atom (PubMed:16820452). {ECO:0000269|PubMed:15240257,
CC       ECO:0000269|PubMed:16820452, ECO:0000269|PubMed:30226766,
CC       ECO:0000305|PubMed:16820452}.
CC   -!- PATHWAY: Hormone biosynthesis. {ECO:0000269|PubMed:30226766}.
CC   -!- INDUCTION: Expression is enhanced at 60 and 90 min after the addition
CC       of the ABA precursor mevalonic acid (MVA) to the medium, but declined
CC       after 120 min. {ECO:0000269|PubMed:16820452}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of abscisic acid (ABA).
CC       {ECO:0000269|PubMed:16820452}.
CC   -!- SIMILARITY: Belongs to the alpha-ionylideneethane synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AM237449; CAJ87067.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q14RS2; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009688; P:abscisic acid biosynthetic process; IDA:GO_Central.
PE   1: Evidence at protein level;
KW   Lyase; Virulence.
FT   CHAIN           1..417
FT                   /note="Alpha-ionylideneethane synthase aba3"
FT                   /id="PRO_0000448432"
SQ   SEQUENCE   417 AA;  47913 MW;  2B624DC7563C544C CRC64;
     MQQVITQTLV DDRFIQISDS KKSEGLATDS TKRQSQEQPI HDKDPIKAAT AAMATTPLVK
     EHQDTWYYPP DIANDLQSIN LPAELKGEIF ACAWEYTRCV IPNYTNWNRY VAFMRIIIMG
     IIAEFRGEMV DVTASNNLLG YDLDATLAAL FEGTPGHKEM AREYKTFLLI TADKASERRD
     GELFRRYVNA LAQSPRHWFR MRDCDALARF TIASALACND LDDIWFTEDQ FEILTEIGDT
     LYDAVAFYKH RAEGETNSTF AYMPEDLRIK AYSECREILW ALDAAWARNP KLANVINFVR
     FFGGPIHMMM RRYRFVEENL TIGKSETDKV VDQTRKNFKL WNRVDANKRS VLNTQRYKAL
     IARSEELMFP GLAEFLEMGG DGICDKCKYR ESTVQNCHTS LVVLNYAANA DYRGEST