ABA4_ARATH
ID ABA4_ARATH Reviewed; 220 AA.
AC Q8LFP9; Q9FZH4;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein ABA DEFICIENT 4, chloroplastic {ECO:0000303|PubMed:17470058};
DE Flags: Precursor;
GN Name=ABA4 {ECO:0000303|PubMed:17470058};
GN OrderedLocusNames=At1g67080 {ECO:0000312|Araport:AT1G67080};
GN ORFNames=F1O19.13 {ECO:0000312|EMBL:AAF98216.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17351115; DOI=10.1105/tpc.106.049114;
RA Dall'Osto L., Cazzaniga S., North H., Marion-Poll A., Bassi R.;
RT "The Arabidopsis aba4-1 mutant reveals a specific function for neoxanthin
RT in protection against photooxidative stress.";
RL Plant Cell 19:1048-1064(2007).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17470058; DOI=10.1111/j.1365-313x.2007.03094.x;
RA North H.M., De Almeida A., Boutin J.P., Frey A., To A., Botran L.,
RA Sotta B., Marion-Poll A.;
RT "The Arabidopsis ABA-deficient mutant aba4 demonstrates that the major
RT route for stress-induced ABA accumulation is via neoxanthin isomers.";
RL Plant J. 50:810-824(2007).
RN [7]
RP INDUCTION BY HEAT STRESS.
RX PubMed=22525244; DOI=10.1016/j.plantsci.2012.03.001;
RA Baron K.N., Schroeder D.F., Stasolla C.;
RT "Transcriptional response of abscisic acid (ABA) metabolism and transport
RT to cold and heat stress applied at the reproductive stage of development in
RT Arabidopsis thaliana.";
RL Plant Sci. 188:48-59(2012).
CC -!- FUNCTION: Required for neoxanthin biosynthesis, an intermediary step in
CC abscisic acid (ABA) biosynthesis. Probably not involved directly in the
CC enzymatic conversion of violaxanthin to neoxanthin. Cannot convert
CC violaxanthin to neoxanthin in vitro. Required for ABA biosynthesis in
CC response to drought stress (PubMed:17470058). Required for neoxanthin
CC biosynthesis which is involved in photoprotection of photosystem II
CC (PSII). Neoxanthin acts as an antioxidant within the photosystem PSII
CC supercomplex (PubMed:17351115). {ECO:0000269|PubMed:17351115,
CC ECO:0000269|PubMed:17470058}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000255};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in root vasculature, root hairs, leaves,
CC trichomes, sepals, stamens, stigma, pedicels, siliques and embryo.
CC {ECO:0000269|PubMed:17470058}.
CC -!- INDUCTION: Induced by heat stress. {ECO:0000269|PubMed:22525244}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants accumulate violaxanthin and completely
CC lack neoxanthin. {ECO:0000269|PubMed:17351115,
CC ECO:0000269|PubMed:17470058}.
CC -!- MISCELLANEOUS: Plants over-expressing ABA4 have increased levels of
CC trans-neoxanthin. {ECO:0000269|PubMed:17470058}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF98216.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007152; AAF98216.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34593.1; -; Genomic_DNA.
DR EMBL; AK175554; BAD43317.1; -; mRNA.
DR EMBL; BT024604; ABD43002.1; -; mRNA.
DR EMBL; AY084717; AAM61291.1; -; mRNA.
DR PIR; F96694; F96694.
DR RefSeq; NP_564889.1; NM_105378.4.
DR AlphaFoldDB; Q8LFP9; -.
DR STRING; 3702.AT1G67080.1; -.
DR iPTMnet; Q8LFP9; -.
DR PaxDb; Q8LFP9; -.
DR PRIDE; Q8LFP9; -.
DR ProteomicsDB; 244545; -.
DR EnsemblPlants; AT1G67080.1; AT1G67080.1; AT1G67080.
DR GeneID; 843028; -.
DR Gramene; AT1G67080.1; AT1G67080.1; AT1G67080.
DR KEGG; ath:AT1G67080; -.
DR Araport; AT1G67080; -.
DR TAIR; locus:2019718; AT1G67080.
DR eggNOG; ENOG502QVVZ; Eukaryota.
DR HOGENOM; CLU_098835_0_0_1; -.
DR InParanoid; Q8LFP9; -.
DR OMA; RHIFQDG; -.
DR OrthoDB; 1588997at2759; -.
DR PhylomeDB; Q8LFP9; -.
DR BioCyc; ARA:AT1G67080-MON; -.
DR PRO; PR:Q8LFP9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LFP9; baseline and differential.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009688; P:abscisic acid biosynthetic process; IMP:TAIR.
DR GO; GO:0032928; P:regulation of superoxide anion generation; IMP:TAIR.
DR GO; GO:0016122; P:xanthophyll metabolic process; IMP:TAIR.
DR InterPro; IPR025461; ABA4-like.
DR Pfam; PF14108; DUF4281; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..37
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 38..220
FT /note="Protein ABA DEFICIENT 4, chloroplastic"
FT /id="PRO_0000435892"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 220 AA; 24624 MW; 0286CA46945F93C1 CRC64;
MGFSSFISQP LSSSLSVMKR NVSAKRSELC LDSSKIRLDH RWSFIGGSRI SVQSNSYTVV
HKKFSGVRAS WLTTTQIASS VFAVGTTAVL PFYTLMVVAP KAEITKKCME SSVPYIILGV
LYVYLLYISW TPETLKYMFS SKYMLPELSG IAKMFSSEMT LASAWIHLLV VDLFAARQVY
NDGLENQIET RHSVSLCLLF CPVGIVSHFV TKAIINNQYK
