ID   RS12_BRUME              Reviewed;         123 AA.
AC   Q8GH23; Q8YHP5;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=30S ribosomal protein S12;
GN   Name=rpsL; OrderedLocusNames=BMEI0752;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LEU-91.
RC   STRAIN=Rev.1;
RX   PubMed=12057611; DOI=10.1016/s0264-410x(02)00159-7;
RA   Cloeckaert A., Grayon M., Grepinet O.;
RT   "Identification of Brucella melitensis vaccine strain Rev.1 by PCR-RFLP
RT   based on a mutation in the rpsL gene.";
RL   Vaccine 20:2546-2550(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. {ECO:0000250}.
CC   -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC       involved in tRNA selection in the A site and with the mRNA backbone.
CC       Located at the interface of the 30S and 50S subunits, it traverses the
CC       body of the 30S subunit contacting proteins on the other side and
CC       probably holding the rRNA structure together. The combined cluster of
CC       proteins S8, S12 and S17 appears to hold together the shoulder and
CC       platform of the 30S subunit (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC       S17. May interact with IF1 in the 30S initiation complex (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL51933.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF448459; AAN77280.1; -; Genomic_DNA.
DR   EMBL; AE008917; AAL51933.1; ALT_INIT; Genomic_DNA.
DR   PIR; AB3346; AB3346.
DR   RefSeq; WP_002964366.1; NZ_GG703780.1.
DR   AlphaFoldDB; Q8GH23; -.
DR   SMR; Q8GH23; -.
DR   STRING; 224914.BMEI0752; -.
DR   EnsemblBacteria; AAL51933; AAL51933; BMEI0752.
DR   GeneID; 45124606; -.
DR   GeneID; 55590913; -.
DR   KEGG; bme:BMEI0752; -.
DR   PATRIC; fig|224914.52.peg.701; -.
DR   eggNOG; COG0048; Bacteria.
DR   OMA; SPALEKC; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_S12/S23.
DR   InterPro; IPR005679; Ribosomal_S12_bac.
DR   PANTHER; PTHR11652; PTHR11652; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   3: Inferred from homology;
KW   Methylation; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding; tRNA-binding.
FT   CHAIN           1..123
FT                   /note="30S ribosomal protein S12"
FT                   /id="PRO_0000146190"
FT   MOD_RES         89
FT                   /note="3-methylthioaspartic acid"
FT                   /evidence="ECO:0000250"
FT   VARIANT         91
FT                   /note="P -> L (in strain: Rev.1; induces streptomycin
FT                   resistance in this live attenuated vaccine strain)"
FT                   /evidence="ECO:0000269|PubMed:12057611"
SQ   SEQUENCE   123 AA;  13871 MW;  183E267BBD72B54B CRC64;
     MPTVNQLIRK PRTAPVKRNK VPALQANPQK RGVCTRVYTT TPKKPNSALR KVAKVRLTNG
     FEVIGYIPGE GHNLQEHSVV MIRGGRVKDL PGVRYHIIRG VLDTQGVKNR KQRRSKYGAK
     RPK