RS12_DROME
ID RS12_DROME Reviewed; 139 AA.
AC P80455; Q0E8F1; Q9VU40;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=40S ribosomal protein S12;
GN Name=RpS12 {ECO:0000312|FlyBase:FBgn0286213}; Synonyms=RpS12E;
GN ORFNames=CG11271 {ECO:0000312|FlyBase:FBgn0286213};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ovary;
RA Greig S.R.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF GLY-97.
RX PubMed=31841522; DOI=10.1371/journal.pgen.1008513;
RA Ji Z., Kiparaki M., Folgado V., Kumar A., Blanco J., Rimesso G., Chuen J.,
RA Liu Y., Zheng D., Baker N.E.;
RT "Drosophila RpS12 controls translation, growth, and cell competition
RT through Xrp1.";
RL PLoS Genet. 15:e1008513-e1008513(2019).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (6.0 ANGSTROMS) OF THE 80S RIBOSOME, AND
RP FUNCTION.
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- FUNCTION: Subunit of the 40S ribosomal complex (PubMed:23636399). In
CC wing imaginal disks, might have a role in translation rate, growth and
CC cell competition, probably through regulation of Xrp1 expression
CC (PubMed:31841522). Might have a role in development and longevity
CC (PubMed:31841522). {ECO:0000269|PubMed:23636399,
CC ECO:0000269|PubMed:31841522}.
CC -!- SUBUNIT: Subunit of the 40S ribosomal complex (PubMed:23636399).
CC {ECO:0000269|PubMed:23636399}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eS12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA61806.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X89659; CAA61806.1; ALT_INIT; mRNA.
DR EMBL; AE014296; AAN11845.1; -; Genomic_DNA.
DR EMBL; AY058531; AAL13760.1; -; mRNA.
DR PIR; S58022; S58022.
DR RefSeq; NP_729866.1; NM_168534.4.
DR PDB; 4V6W; EM; 6.00 A; AM=1-139.
DR PDB; 6XU6; EM; 3.50 A; AM=21-139.
DR PDB; 6XU7; EM; 4.90 A; AM=21-139.
DR PDB; 6XU8; EM; 3.00 A; AM=21-139.
DR PDBsum; 4V6W; -.
DR PDBsum; 6XU6; -.
DR PDBsum; 6XU7; -.
DR PDBsum; 6XU8; -.
DR AlphaFoldDB; P80455; -.
DR SMR; P80455; -.
DR BioGRID; 64826; 110.
DR IntAct; P80455; 4.
DR STRING; 7227.FBpp0075612; -.
DR PaxDb; P80455; -.
DR PRIDE; P80455; -.
DR DNASU; 39480; -.
DR EnsemblMetazoa; FBtr0075878; FBpp0075612; FBgn0286213.
DR GeneID; 39480; -.
DR KEGG; dme:Dmel_CG11271; -.
DR CTD; 6206; -.
DR FlyBase; FBgn0286213; RpS12.
DR VEuPathDB; VectorBase:FBgn0286213; -.
DR eggNOG; KOG3406; Eukaryota.
DR GeneTree; ENSGT00390000018318; -.
DR HOGENOM; CLU_110343_1_0_1; -.
DR InParanoid; P80455; -.
DR OMA; CDEPMYK; -.
DR OrthoDB; 1474866at2759; -.
DR PhylomeDB; P80455; -.
DR Reactome; R-DME-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-DME-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-DME-72649; Translation initiation complex formation.
DR Reactome; R-DME-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-DME-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-DME-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-DME-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-DME-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 39480; 1 hit in 1 CRISPR screen.
DR ChiTaRS; RpS12; fly.
DR GenomeRNAi; 39480; -.
DR PRO; PR:P80455; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0286213; Expressed in seminal fluid secreting gland and 38 other tissues.
DR Genevisible; P80455; DM.
DR GO; GO:0022626; C:cytosolic ribosome; IDA:FlyBase.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:FlyBase.
DR GO; GO:0002181; P:cytoplasmic translation; TAS:FlyBase.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IMP:FlyBase.
DR Gene3D; 3.30.1330.30; -; 1.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR InterPro; IPR000530; Ribosomal_S12e.
DR PANTHER; PTHR11843; PTHR11843; 1.
DR Pfam; PF01248; Ribosomal_L7Ae; 1.
DR PRINTS; PR00972; RIBSOMALS12E.
DR SUPFAM; SSF55315; SSF55315; 1.
DR PROSITE; PS01189; RIBOSOMAL_S12E; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..139
FT /note="40S ribosomal protein S12"
FT /id="PRO_0000122330"
FT MUTAGEN 97
FT /note="G->D: Moderately delays development and reduces
FT lifespan. In wing imaginal disks, increases its transcript
FT level."
FT /evidence="ECO:0000269|PubMed:23636399"
SQ SEQUENCE 139 AA; 15169 MW; 4810D50ADC87C253 CRC64;
MADVDVDVPS AAPVLDGAMD INTALQEVLK KSLIADGLVH GIHQACKALD KRQAVLCILA
ESFDEPNYKK LVTALCNEHQ IPLIRVDSHK KLGEWSGLCK IDKEGKPRKV CGCSVVVIKD
FGEETPALDV VKDHLRQNS
