RS12_ENTFA
ID RS12_ENTFA Reviewed; 137 AA.
AC Q839H1;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=30S ribosomal protein S12 {ECO:0000255|HAMAP-Rule:MF_00403};
GN Name=rpsL {ECO:0000255|HAMAP-Rule:MF_00403}; OrderedLocusNames=EF_0198;
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583;
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
CC -!- FUNCTION: With S4 and S5 plays an important role in translational
CC accuracy. {ECO:0000255|HAMAP-Rule:MF_00403}.
CC -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC involved in tRNA selection in the A site and with the mRNA backbone.
CC Located at the interface of the 30S and 50S subunits, it traverses the
CC body of the 30S subunit contacting proteins on the other side and
CC probably holding the rRNA structure together. The combined cluster of
CC proteins S8, S12 and S17 appears to hold together the shoulder and
CC platform of the 30S subunit. {ECO:0000255|HAMAP-Rule:MF_00403}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC S17. May interact with IF1 in the 30S initiation complex.
CC {ECO:0000255|HAMAP-Rule:MF_00403}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000255|HAMAP-Rule:MF_00403}.
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DR EMBL; AE016830; AAO80068.1; -; Genomic_DNA.
DR RefSeq; NP_813997.1; NC_004668.1.
DR RefSeq; WP_002356190.1; NZ_KE136524.1.
DR PDB; 6WUB; EM; 3.20 A; l=2-137.
DR PDB; 7P7R; EM; 2.90 A; m=1-137.
DR PDBsum; 6WUB; -.
DR PDBsum; 7P7R; -.
DR AlphaFoldDB; Q839H1; -.
DR SMR; Q839H1; -.
DR STRING; 226185.EF_0198; -.
DR EnsemblBacteria; AAO80068; AAO80068; EF_0198.
DR GeneID; 60892694; -.
DR KEGG; efa:EF0198; -.
DR PATRIC; fig|226185.45.peg.68; -.
DR eggNOG; COG0048; Bacteria.
DR HOGENOM; CLU_104295_1_1_9; -.
DR OMA; SPALEKC; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methylation; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; tRNA-binding.
FT CHAIN 1..137
FT /note="30S ribosomal protein S12"
FT /id="PRO_0000146223"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 102
FT /note="3-methylthioaspartic acid"
FT /evidence="ECO:0000250"
FT HELIX 4..9
FT /evidence="ECO:0007829|PDB:6WUB"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 43..53
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 63..70
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 92..97
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 106..110
FT /evidence="ECO:0007829|PDB:6WUB"
FT STRAND 114..117
FT /evidence="ECO:0007829|PDB:6WUB"
FT TURN 127..131
FT /evidence="ECO:0007829|PDB:6WUB"
SQ SEQUENCE 137 AA; 15277 MW; EF6DE7883EF8DB56 CRC64;
MPTINQLVRK PRKSKVEKSD SPALNKGYNS FKKTQTNVNS PQKRGVCTRV GTMTPKKPNS
ALRKYARVRL SNLIEVTAYI PGIGHNLQEH SVVLLRGGRV KDLPGVRYHI VRGALDTAGV
NDRKQSRSKY GTKRPKA
