RS12_HAEIN
ID RS12_HAEIN Reviewed; 124 AA.
AC P63196; P44412;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=30S ribosomal protein S12;
DE AltName: Full=Streptomycin resistance protein;
GN Name=rpsL; Synonyms=rps12, strA; OrderedLocusNames=HI_0581;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS STREPTOMYCIN RESISTANT
RP ARG-43 AND VAL-81.
RC STRAIN=Rd / BC200;
RX PubMed=1512195; DOI=10.1128/jb.174.17.5604-5608.1992;
RA Stuy J.H., Walter R.B.;
RT "Cloning, characterization, and DNA base sequence of the high-level
RT streptomycin resistance gene strA1 of Haemophilus influenzae Rd.";
RL J. Bacteriol. 174:5604-5608(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: With S4 and S5 plays an important role in translational
CC accuracy. {ECO:0000250}.
CC -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC involved in tRNA selection in the A site and with the mRNA backbone.
CC Located at the interface of the 30S and 50S subunits, it traverses the
CC body of the 30S subunit contacting proteins on the other side and
CC probably holding the rRNA structure together. The combined cluster of
CC proteins S8, S12 and S17 appears to hold together the shoulder and
CC platform of the 30S subunit (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC S17. May interact with IF1 in the 30S initiation complex (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
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DR EMBL; M86701; AAA25003.1; -; Genomic_DNA.
DR EMBL; M86702; AAA25004.1; -; Genomic_DNA.
DR EMBL; L42023; AAC22239.1; -; Genomic_DNA.
DR PIR; B42939; A42939.
DR RefSeq; NP_438739.1; NC_000907.1.
DR RefSeq; WP_005543325.1; NC_000907.1.
DR AlphaFoldDB; P63196; -.
DR SMR; P63196; -.
DR STRING; 71421.HI_0581; -.
DR EnsemblBacteria; AAC22239; AAC22239; HI_0581.
DR GeneID; 49634547; -.
DR KEGG; hin:HI_0581; -.
DR PATRIC; fig|71421.8.peg.602; -.
DR eggNOG; COG0048; Bacteria.
DR HOGENOM; CLU_104295_1_2_6; -.
DR OMA; SPALEKC; -.
DR PhylomeDB; P63196; -.
DR BioCyc; HINF71421:G1GJ1-594-MON; -.
DR PRO; PR:P63196; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Methylation; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; tRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..124
FT /note="30S ribosomal protein S12"
FT /id="PRO_0000146232"
FT MOD_RES 89
FT /note="3-methylthioaspartic acid"
FT /evidence="ECO:0000250"
FT VARIANT 43
FT /note="K -> R (in streptomycin resistant strA1)"
FT /evidence="ECO:0000269|PubMed:1512195"
FT VARIANT 81
FT /note="L -> V (in streptomycin resistant stra1)"
FT /evidence="ECO:0000269|PubMed:1512195"
SQ SEQUENCE 124 AA; 13749 MW; D51C8A7BDD4E1D45 CRC64;
MATINQLVRK PRVKKVVKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRIRLTNG
FEVTSYIGGE GHNLQEHSVV LIRGGRVKDL PGVRYHTVRG ALDCAGVKDR KQGRSKYGVK
RPKA
