RS12_MYCTU
ID RS12_MYCTU Reviewed; 124 AA.
AC P9WH63; L0T4E7; P41196; Q933X1; Q93MR7; Q93MR8; Q93T44;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=30S ribosomal protein S12;
GN Name=rpsL; Synonyms=rps12; OrderedLocusNames=Rv0682; ORFNames=MTV040.10;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS STREPTOMYCIN RESISTANT.
RX PubMed=7934937; DOI=10.1111/j.1365-2958.1993.tb01253.x;
RA Finken M., Kirschner P., Meier A., Wrede A., Boettger E.C.;
RT "Molecular basis of streptomycin resistance in Mycobacterium tuberculosis:
RT alterations of the ribosomal protein S12 gene and point mutations within a
RT functional 16S ribosomal RNA pseudoknot.";
RL Mol. Microbiol. 9:1239-1246(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=7968530; DOI=10.1111/j.1365-2958.1993.tb00924.x;
RA Nair J., Rouse D.A., Bai G.H., Morris S.L.;
RT "The rpsL gene and streptomycin resistance in single and multiple drug-
RT resistant strains of Mycobacterium tuberculosis.";
RL Mol. Microbiol. 10:521-527(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=8192450; DOI=10.1128/aac.38.2.238;
RA Honore N., Cole S.T.;
RT "Streptomycin resistance in mycobacteria.";
RL Antimicrob. Agents Chemother. 38:238-242(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=An03, F05, F12, and F18;
RA Orru G., Iona E., Vicidomini S., Oggioni M.R., Fattorini L., Orefici G.,
RA Pozzi G.;
RT "Mutations associated to streptomycin resistant strains of Mycobacterium
RT tuberculosis isolated in Italy.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [7]
RP VARIANTS STREPTOMYCIN RESISTANT.
RC STRAIN=C37, C9, TCVGH1, TCVGH21, and TCVGH25;
RA Shi Z.-Y., Lee K., Hu C.-H., Liu M.-F.;
RT "The allelic profiles of multidrug-resistant Mycobacterium tuberculosis
RT isolates from Taiwan.";
RL Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-104, AND VARIANT THR-43.
RA Hainan H., Jinxiang H., Changzheng S.S.;
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: With S4 and S5 plays an important role in translational
CC accuracy. {ECO:0000250}.
CC -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC involved in tRNA selection in the A site and with the mRNA backbone.
CC Located at the interface of the 30S and 50S subunits, it traverses the
CC body of the 30S subunit contacting proteins on the other side and
CC probably holding the rRNA structure together. The combined cluster of
CC proteins S8, S12 and S17 appears to hold together the shoulder and
CC platform of the 30S subunit (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC S17. May interact with IF1 in the 30S initiation complex (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
CC -!- CAUTION: Because the enzyme that would modify Asp-89 to 3-
CC methylthioaspartic acid has not been found in the proteome of this
CC organism, that modification is not predicted. {ECO:0000305}.
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DR EMBL; X70995; CAA50323.1; -; Genomic_DNA.
DR EMBL; L08011; AAA66176.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP43425.1; -; Genomic_DNA.
DR EMBL; AF398879; AAK83385.1; -; Genomic_DNA.
DR EMBL; AF398880; AAK83386.1; -; Genomic_DNA.
DR EMBL; AF398881; AAK83387.1; -; Genomic_DNA.
DR EMBL; AF398882; AAK83388.1; -; Genomic_DNA.
DR EMBL; AY156729; AAN52761.1; -; Genomic_DNA.
DR EMBL; AY156730; AAN52762.1; -; Genomic_DNA.
DR EMBL; AY156732; AAN52764.1; -; Genomic_DNA.
DR EMBL; AY156733; AAN52765.1; -; Genomic_DNA.
DR EMBL; AF367438; AAK53753.1; -; Genomic_DNA.
DR PIR; S39591; S39591.
DR RefSeq; NP_215196.1; NC_000962.3.
DR RefSeq; WP_003403453.1; NZ_NVQJ01000007.1.
DR PDB; 7KGB; EM; 2.70 A; l=1-124.
DR PDB; 7MSC; EM; 2.97 A; l=1-124.
DR PDB; 7MSH; EM; 3.23 A; l=1-124.
DR PDB; 7MSM; EM; 2.79 A; l=1-124.
DR PDB; 7MSZ; EM; 3.10 A; l=1-124.
DR PDB; 7MT2; EM; 2.76 A; l=1-124.
DR PDB; 7MT3; EM; 2.80 A; l=1-124.
DR PDB; 7MT7; EM; 2.71 A; l=1-124.
DR PDBsum; 7KGB; -.
DR PDBsum; 7MSC; -.
DR PDBsum; 7MSH; -.
DR PDBsum; 7MSM; -.
DR PDBsum; 7MSZ; -.
DR PDBsum; 7MT2; -.
DR PDBsum; 7MT3; -.
DR PDBsum; 7MT7; -.
DR AlphaFoldDB; P9WH63; -.
DR SMR; P9WH63; -.
DR STRING; 83332.Rv0682; -.
DR PaxDb; P9WH63; -.
DR DNASU; 888259; -.
DR GeneID; 45424644; -.
DR GeneID; 888259; -.
DR KEGG; mtu:Rv0682; -.
DR TubercuList; Rv0682; -.
DR eggNOG; COG0048; Bacteria.
DR OMA; SPALEKC; -.
DR PhylomeDB; P9WH63; -.
DR PRO; PR:P9WH63; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; tRNA-binding.
FT CHAIN 1..124
FT /note="30S ribosomal protein S12"
FT /id="PRO_0000146272"
FT REGION 105..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 43
FT /note="K -> R (in strain: 9106, 9181, An03, F12, F18 and
FT TCVGH21; resistant to streptomycin)"
FT VARIANT 43
FT /note="K -> T (in strain: TCVGH25; probably resistant to
FT streptomycin)"
FT /evidence="ECO:0000269|Ref.8"
FT VARIANT 88
FT /note="K -> Q (in strain: F05; resistant to streptomycin)"
FT VARIANT 88
FT /note="K -> R (in strain: C37; probably resistant to
FT streptomycin)"
FT VARIANT 88
FT /note="K -> T (in strain: F18; resistant to streptomycin)"
SQ SEQUENCE 124 AA; 13849 MW; 2B441B040CEF20CD CRC64;
MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR KVARVKLTSQ
VEVTAYIPGE GHNLQEHSMV LVRGGRVKDL PGVRYKIIRG SLDTQGVKNR KQARSRYGAK
KEKG
