ABAA_EMENI
ID ABAA_EMENI Reviewed; 796 AA.
AC P20945; C8VTG3; Q5BGA8;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 3.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Conidiophore development regulator abaA {ECO:0000305};
GN Name=abaA {ECO:0000303|PubMed:2655931}; ORFNames=AN0422;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, AND FUNCTION.
RC STRAIN=FGSC 26;
RX PubMed=2655931; DOI=10.1016/0092-8674(89)90800-3;
RA Mirabito P.M., Adams T.H., Timberlake W.E.;
RT "Interactions of three sequentially expressed genes control temporal and
RT spatial specificity in Aspergillus development.";
RL Cell 57:859-868(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=2823119; DOI=10.1128/mcb.7.9.3113-3118.1987;
RA Boylan M.T., Mirabito P.M., Willett C.E., Zimmerman C.R., Timberlake W.E.;
RT "Isolation and physical characterization of three essential conidiation
RT genes from Aspergillus nidulans.";
RL Mol. Cell. Biol. 7:3113-3118(1987).
RN [5]
RP INDUCTION.
RX PubMed=2117702; DOI=10.1128/mcb.10.9.4912-4919.1990;
RA Adams T.H., Timberlake W.E.;
RT "Upstream elements repress premature expression of an Aspergillus
RT developmental regulatory gene.";
RL Mol. Cell. Biol. 10:4912-4919(1990).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=2152124; DOI=10.2307/3869172;
RA Sewall T.C., Mims C.W., Timberlake W.E.;
RT "abaA controls phialide differentiation in Aspergillus nidulans.";
RL Plant Cell 2:731-739(1990).
RN [7]
RP FUNCTION.
RX PubMed=2196567; DOI=10.1073/pnas.87.14.5405;
RA Adams T.H., Timberlake W.E.;
RT "Developmental repression of growth and gene expression in Aspergillus.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:5405-5409(1990).
RN [8]
RP DOMAIN TEA.
RX PubMed=2070413; DOI=10.1016/0092-8674(91)90132-i;
RA Buerglin T.R.;
RT "The TEA domain: a novel, highly conserved DNA-binding motif.";
RL Cell 66:11-12(1991).
RN [9]
RP FUNCTION.
RX PubMed=7704830; DOI=10.1139/m94-164;
RA Sewall T.C.;
RT "Cellular effects of misscheduled brlA, abaA, and wetA expression in
RT Aspergillus nidulans.";
RL Can. J. Microbiol. 40:1035-1042(1994).
RN [10]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=8139553; DOI=10.1128/mcb.14.4.2503-2515.1994;
RA Andrianopoulos A., Timberlake W.E.;
RT "The Aspergillus nidulans abaA gene encodes a transcriptional activator
RT that acts as a genetic switch to control development.";
RL Mol. Cell. Biol. 14:2503-2515(1994).
RN [11]
RP INDUCTION.
RX PubMed=24587098; DOI=10.1371/journal.pone.0089883;
RA Park H.S., Nam T.Y., Han K.H., Kim S.C., Yu J.H.;
RT "VelC positively controls sexual development in Aspergillus nidulans.";
RL PLoS ONE 9:E89883-E89883(2014).
CC -!- FUNCTION: BrlA, abaA and wetA are pivotal regulators of conidiophore
CC development and conidium maturation (PubMed:2655931, PubMed:2196567).
CC They act individually and together to regulate their own expression and
CC that of numerous other sporulation-specific gene (PubMed:2655931,
CC PubMed:2823119). Controls temporal and spatial specificity in
CC Aspergillus development (PubMed:2655931, PubMed:7704830). Directs the
CC differentiation of phialides and is continuously required for
CC maintenance of their function (PubMed:2152124). Expression of abaA
CC leads to activation of brlA and wetA, cessation of vegetative growth,
CC and accentuated cellular vacuolization (PubMed:2655931). Binds to the
CC sequence 5'-CATTCY-3', where Y is a pyrimidine, making both major- and
CC minor-groove contacts (PubMed:8139553). Multiple abaA binding sites are
CC present in the cis-acting regulatory regions of several developmentally
CC controlled structural genes as well as those of the upstream regulatory
CC gene brlA, the downstream regulatory gene wetA, and abaA itself
CC (PubMed:8139553). {ECO:0000269|PubMed:2152124,
CC ECO:0000269|PubMed:2196567, ECO:0000269|PubMed:2655931,
CC ECO:0000269|PubMed:2823119, ECO:0000269|PubMed:7704830,
CC ECO:0000269|PubMed:8139553}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:I1S4T3}.
CC Note=localizes to the nuclei of phialides and terminal cells of mature
CC conidia (By similarity). {ECO:0000250|UniProtKB:I1S4T3}.
CC -!- INDUCTION: Expression is induced during conidiation, after conidiophore
CC vesicles, metullae, and phialides had formed (PubMed:2823119).
CC Negatively regulated by velC (PubMed:24587098). In the promoter, a 45-
CC base-pair region encompassing the major and minor abaA transcription
CC initiation sites contains directly repeated sequences related to the
CC mammalian initiator (Inr) element and is sufficient for correct
CC transcription initiation and for developmental induction
CC (PubMed:2117702). {ECO:0000269|PubMed:2117702,
CC ECO:0000269|PubMed:24587098, ECO:0000269|PubMed:2823119}.
CC -!- DISRUPTION PHENOTYPE: Prevents the formation of normal conidiophores
CC (PubMed:2823119, PubMed:2152124). Fails to form any viable conidia,
CC even though it does produce morphologically normal
CC phialides(PubMed:2655931). {ECO:0000269|PubMed:2152124,
CC ECO:0000269|PubMed:2655931, ECO:0000269|PubMed:2823119}.
CC -!- SIMILARITY: Belongs to the TEC1 family. {ECO:0000305}.
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DR EMBL; J04850; AAA33286.1; -; Genomic_DNA.
DR EMBL; AACD01000007; EAA66521.1; -; Genomic_DNA.
DR EMBL; BN001308; CBF89518.1; -; Genomic_DNA.
DR PIR; A32434; A32434.
DR RefSeq; XP_658026.1; XM_652934.1.
DR AlphaFoldDB; P20945; -.
DR SMR; P20945; -.
DR STRING; 162425.CADANIAP00002276; -.
DR EnsemblFungi; CBF89518; CBF89518; ANIA_00422.
DR EnsemblFungi; EAA66521; EAA66521; AN0422.2.
DR GeneID; 2876199; -.
DR KEGG; ani:AN0422.2; -.
DR VEuPathDB; FungiDB:AN0422; -.
DR eggNOG; KOG3841; Eukaryota.
DR HOGENOM; CLU_356362_0_0_1; -.
DR InParanoid; P20945; -.
DR OMA; MWVSAPQ; -.
DR OrthoDB; 258258at2759; -.
DR Proteomes; UP000000560; Chromosome VIII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:AspGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0070787; P:conidiophore development; IMP:CACAO.
DR GO; GO:0048315; P:conidium formation; IMP:AspGD.
DR GO; GO:0070790; P:phialide development; IMP:AspGD.
DR GO; GO:0075307; P:positive regulation of conidium formation; IMP:AspGD.
DR GO; GO:0070807; P:positive regulation of phialide development; IMP:AspGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:AspGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:AspGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 6.10.20.40; -; 1.
DR InterPro; IPR000818; TEA/ATTS_dom.
DR InterPro; IPR038096; TEA/ATTS_sf.
DR Pfam; PF01285; TEA; 1.
DR PRINTS; PR00065; TEADOMAIN.
DR SMART; SM00426; TEA; 1.
DR PROSITE; PS00554; TEA_1; 1.
DR PROSITE; PS51088; TEA_2; 1.
PE 1: Evidence at protein level;
KW Activator; Conidiation; DNA-binding; Nucleus; Reference proteome;
KW Sporulation; Transcription; Transcription regulation.
FT CHAIN 1..796
FT /note="Conidiophore development regulator abaA"
FT /id="PRO_0000205940"
FT DNA_BIND 133..207
FT /note="TEA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00505"
FT REGION 215..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 341..362
FT /note="Leucine-zipper-like"
FT REGION 612..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..643
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 631
FT /note="T -> I (in Ref. 1; AAA33286)"
FT /evidence="ECO:0000305"
FT CONFLICT 641
FT /note="I -> T (in Ref. 1; AAA33286)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 796 AA; 89171 MW; 934AC9510AAA1CCE CRC64;
MATDWQPECM VSQNQAALES IGAHSDRALQ NTSGNVQAYS DSLAHHDTTG RDDPLQHYTL
KYPHPPVPVP SHPLPTATAN LYHPQLLSHR YQVKKLRRLQ SNGSSYAGSR RGRSYLKSQK
YLEYRARPRR DTGKDGEPVW SDELEDAFQQ ALEANPPMGR RKWSERGKSY GRNELIAEYI
YKLTGKRRTR KQVSSHLQVL DSFLKGDPDW ERLVREQSDR STAQTQPVGP RWRTSMDHLP
SSHYGTHATS SYPEPMRLMP PYSADLQLPQ YSPTSTQQDT NNNTIQGLSF DMWVSAPNKP
DRIDDAYHLY TRLQGDQRQP PMPLEDLKNW RVSFPHLSSS LSDVNDPLNC EIILLETNLE
LMDDFPPMGS RLGIHLELDI ANPMSGTAPT VNQMENWTCS TYIYEDGRRT MEAYHNLTKP
HTTKVKPPFE SSWWAKTFTK LTQDKREAES TGHHHAADER TRRYFHSLTA VQEIRATVPP
SLRRLQNHYP GSPAEESKRM AIILWKFRQT RPNEVGTTTW RKLITSPDRA LTNSPRPSTA
IDLPPLSLDS ILLSKPTSNL YQAPPQHHDL LHQNAPSQQS WSLYQPSHDH VNSLYHSAGA
FDFLNSITKP EEGLSDKTAP TSVLDPFPNL TQQTTSQTAG INVSSGTPVM LQIPDLSLSS
NLGTYGLGHE SHYVPSHHNA ANLHDHSSTT GLGHYFAPST QSLDEISHSH APWSAPNTTI
SGDTSGGNYH HLPFTTSDHS VTVSRESHQN HSFEGLLPSD DLVGIVGGLS GDPNMNGAGP
EHTSSAYAEH TAVEAV
