ID   RS12_STRFL              Reviewed;         123 AA.
AC   P0A4A6; P97222;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=30S ribosomal protein S12;
GN   Name=rpsL;
OS   Streptomyces filamentosus (Streptomyces roseosporus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=67294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31568 / A21978.6 / NRRL 11379;
RX   PubMed=8981996; DOI=10.1128/jb.179.1.180-186.1997;
RA   Hosted T.J., Baltz R.H.;
RT   "Use of rpsL for dominance selection and gene replacement in Streptomyces
RT   roseosporus.";
RL   J. Bacteriol. 179:180-186(1997).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. {ECO:0000250}.
CC   -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC       involved in tRNA selection in the A site and with the mRNA backbone.
CC       Located at the interface of the 30S and 50S subunits, it traverses the
CC       body of the 30S subunit contacting proteins on the other side and
CC       probably holding the rRNA structure together. The combined cluster of
CC       proteins S8, S12 and S17 appears to hold together the shoulder and
CC       platform of the 30S subunit (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC       S17. May interact with IF1 in the 30S initiation complex (By
CC       similarity). {ECO:0000250}.
CC   -!- BIOTECHNOLOGY: One streptomycin resistant strain of S.coelicolor and
CC       S.lividans (K88E) produces increased quantities of the natural
CC       antibiotic actinorhodin; strains which are resistant to multiple drugs
CC       produce more antibiotic.
CC   -!- MISCELLANEOUS: The streptomycin sensitive allele is dominant to the
CC       resistant allele.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U60191; AAC44743.1; -; Genomic_DNA.
DR   RefSeq; WP_003948652.1; NZ_PDCL01000001.1.
DR   AlphaFoldDB; P0A4A6; -.
DR   SMR; P0A4A6; -.
DR   GeneID; 58470329; -.
DR   GeneID; 63982115; -.
DR   GeneID; 67401280; -.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_S12/S23.
DR   InterPro; IPR005679; Ribosomal_S12_bac.
DR   PANTHER; PTHR11652; PTHR11652; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Methylation; Ribonucleoprotein; Ribosomal protein;
KW   RNA-binding; rRNA-binding; tRNA-binding.
FT   CHAIN           1..123
FT                   /note="30S ribosomal protein S12"
FT                   /id="PRO_0000146321"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         89
FT                   /note="3-methylthioaspartic acid"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   123 AA;  13771 MW;  9F9C1621AD40D5A0 CRC64;
     MPTIQQLVRK GRQDKVEKNK TPALEGSPQR RGVCTRVFTT TPKKPNSALR KVARVRLTSG
     IEVTAYIPGE GHNLQEHSIV LVRGGRVKDL PGVRYKIIRG SLDTQGVKNR KQARSRYGAK
     KEK