RS12_SYNE7
ID RS12_SYNE7 Reviewed; 124 AA.
AC P63200; P18662; Q31PV2;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=30S ribosomal protein S12;
GN Name=rpsL; Synonyms=rps12; OrderedLocusNames=Synpcc7942_0887;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT STREPTOMYCIN RESISTANT.
RX PubMed=11495986; DOI=10.1099/00221287-147-8-2077;
RA Matsuoka M., Takahama K., Ogawa T.;
RT "Gene replacement in cyanobacteria mediated by a dominant streptomycin-
RT sensitive rps12 gene that allows selection of mutants free from drug
RT resistance markers.";
RL Microbiology 147:2077-2087(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: With S4 and S5 plays an important role in translational
CC accuracy. {ECO:0000250}.
CC -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC involved in tRNA selection in the A site and with the mRNA backbone.
CC Located at the interface of the 30S and 50S subunits, it traverses the
CC body of the 30S subunit contacting proteins on the other side and
CC probably holding the rRNA structure together. The combined cluster of
CC proteins S8, S12 and S17 appears to hold together the shoulder and
CC platform of the 30S subunit (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC S17. May interact with IF1 in the 30S initiation complex (By
CC similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: The streptomycin sensitive allele is dominant to the
CC resistant allele.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
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DR EMBL; CP000100; ABB56917.1; -; Genomic_DNA.
DR RefSeq; WP_011242965.1; NC_007604.1.
DR AlphaFoldDB; P63200; -.
DR SMR; P63200; -.
DR STRING; 1140.Synpcc7942_0887; -.
DR PRIDE; P63200; -.
DR EnsemblBacteria; ABB56917; ABB56917; Synpcc7942_0887.
DR KEGG; syf:Synpcc7942_0887; -.
DR eggNOG; COG0048; Bacteria.
DR HOGENOM; CLU_104295_1_2_3; -.
DR OMA; SPALEKC; -.
DR OrthoDB; 1707228at2; -.
DR BioCyc; SYNEL:SYNPCC7942_0887-MON; -.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Methylation; Ribonucleoprotein; Ribosomal protein;
KW RNA-binding; rRNA-binding; tRNA-binding.
FT CHAIN 1..124
FT /note="30S ribosomal protein S12"
FT /id="PRO_0000146336"
FT REGION 105..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 89
FT /note="3-methylthioaspartic acid"
FT /evidence="ECO:0000250"
FT VARIANT 43
FT /note="K -> R (in allele RPS12-R43; confers streptomycin
FT resistance)"
SQ SEQUENCE 124 AA; 13991 MW; 0412723637E0561E CRC64;
MPTIQQLIRD EREKITKKTK SPALKNCPQR RGVCTRVYTT TPKKPNSALR KVARVRLTSG
FEVTAYIPGI GHNLQEHSVV MIRGGRVKDL PGVRYHIIRG TLDTAGVKDR RQSRSKYGAK
RPKA
