ID   AZPB6_ASPTN             Reviewed;         601 AA.
AC   Q0CF68;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Transcription factor ATEG_07666 {ECO:0000303|PubMed:31908094};
DE   AltName: Full=Azasperpyranone A biosynthesis cluster B protein ATEG_07666 {ECO:0000303|PubMed:31908094};
GN   ORFNames=ATEG_07666;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND BIOTECHNOLOGY.
RX   PubMed=31908094; DOI=10.1002/anie.201915514;
RA   Huang X., Zhang W., Tang S., Wei S., Lu X.;
RT   "Collaborative biosynthesis of a class of bioactive azaphilones by two
RT   separate gene clusters containing four PKS/NRPSs with transcriptional
RT   cosstalk in fungi.";
RL   Angew. Chem. Int. Ed. 59:4349-4353(2020).
CC   -!- FUNCTION: Specific transcriptional regulator for the azasperpyranone A
CC       biosynthesis cluster B. {ECO:0000269|PubMed:31908094}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- INDUCTION: Expression is induced by the azasperpyranone transcriptional
CC       regulator ATEG_07667. {ECO:0000269|PubMed:31908094}.
CC   -!- DISRUPTION PHENOTYPE: Results in the significant down-regulation of
CC       cluster B, whereas it exerts no influence on cluster A.
CC       {ECO:0000269|PubMed:31908094}.
CC   -!- BIOTECHNOLOGY: Azasperpyranones display potential anti-cancer
CC       activities (PubMed:31908094). Azasperpyranones A, C, D, and F exhibit
CC       potent growth-inhibitory activity against the A549, HepG2, HCT-116, and
CC       HL-60 cell lines, with IC(50) values of 2.39-14.42 mm, respectively
CC       (PubMed:31908094). Moreover, azasperpyranone D significantly inhibits
CC       HCT-116 xenograft tumor growth in BALB/c-nu mice (PubMed:31908094). In
CC       addition, azasperpyranones A and C can bind with four kinds of
CC       therapeutic targets for cancer, eEF2K, FGFR, survivin, and TNF-a
CC       (PubMed:31908094). {ECO:0000269|PubMed:31908094}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAU31928.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; CH476604; EAU31928.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001216287.1; XM_001216287.1.
DR   AlphaFoldDB; Q0CF68; -.
DR   EnsemblFungi; EAU31928; EAU31928; ATEG_07666.
DR   GeneID; 4322611; -.
DR   eggNOG; ENOG502RZ01; Eukaryota.
DR   HOGENOM; CLU_011017_3_1_1; -.
DR   OrthoDB; 455010at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..601
FT                   /note="Transcription factor ATEG_07666"
FT                   /id="PRO_0000450092"
FT   DNA_BIND        17..44
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
SQ   SEQUENCE   601 AA;  66402 MW;  06DEA20BFE95CCB0 CRC64;
     MSCPAARKGR QQPGFACEEC RRRKARCDRV RPKCGFCTEN GMQCVIVEKR QQRGPIKGQL
     NSMQSQLELP AGIPPVLDDF DIQASGLSEH DMSLVAATSV GTSTEASLIL PAALDKLELP
     ASSEAAAAGL PGWPEWLDWQ DMNNSSLILP ELTASTLTDP LSLDSLNGSP PTMVDSSSKI
     QMTDLIRAEL DQLYFDRVHA FCPIIHRRRY FASLAQDNHT TAQLCLQSAM RALAAAMSAN
     SSHLSEQFYS EARSLLDTHS QTPATPRDKI PLEHIQAWLL LSHYELLRIG VHQAMLTAGR
     AFRLVQMARL CEIDAPGGDL QLSPVASSSS SISTNTDSSE SFVDAEEGRR TFWKKWHVFL
     HADTMQNSLT RLLPIQVSTR LPAPEANFQN NQPTRTSFLA DAMAQTGPST LSPFAECIIM
     ATLHGRCMTH RRLYATEFEA GTRDFCIRQG WLATAVERRV QMLVPSPAVD SDPMLLFTHM
     LAYRATVHLS ITVQQASWRT VDQQVLAAAY QQRAAQAASE IVRLAKAVPY LSPFKTHPFL
     PDTLACAATF LSTQTATDGG GDDIQHLLRV LGELQDTHSL ARDYLQTLKL QVQGNRQDGW
     Y