ID   AZPB7_ASPTN             Reviewed;         593 AA.
AC   Q0CF67;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Transcription factor ATEG_07667 {ECO:0000303|PubMed:31908094};
DE   AltName: Full=Azasperpyranone A biosynthesis cluster B protein ATEG_07667 {ECO:0000303|PubMed:31908094};
GN   ORFNames=ATEG_07667;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND BIOTECHNOLOGY.
RX   PubMed=31908094; DOI=10.1002/anie.201915514;
RA   Huang X., Zhang W., Tang S., Wei S., Lu X.;
RT   "Collaborative biosynthesis of a class of bioactive azaphilones by two
RT   separate gene clusters containing four PKS/NRPSs with transcriptional
RT   cosstalk in fungi.";
RL   Angew. Chem. Int. Ed. 59:4349-4353(2020).
CC   -!- FUNCTION: Transcriptional regulator that regulates both the
CC       azasperpyranone A biosynthesis clusters A and B (PubMed:31908094).
CC       Specifically up-regulates the expression of the cluster A and B
CC       specific transcription factors ATEG_03638 and ATEG_07666, which in turn
CC       activate the expression of their respective clusters (PubMed:31908094).
CC       {ECO:0000269|PubMed:31908094}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Results in the significant down-regulation of
CC       both azasperpyranone A biosynthesis clusters A and B, including the
CC       cluster-specific transcription factors ATEG_03638 and ATEG_07666.
CC       {ECO:0000269|PubMed:31908094}.
CC   -!- BIOTECHNOLOGY: Azasperpyranones display potential anti-cancer
CC       activities (PubMed:31908094). Azasperpyranones A, C, D, and F exhibit
CC       potent growth-inhibitory activity against the A549, HepG2, HCT-116, and
CC       HL-60 cell lines, with IC(50) values of 2.39-14.42 mm, respectively
CC       (PubMed:31908094). Moreover, azasperpyranone D significantly inhibits
CC       HCT-116 xenograft tumor growth in BALB/c-nu mice (PubMed:31908094). In
CC       addition, azasperpyranones A and C can bind with four kinds of
CC       therapeutic targets for cancer, eEF2K, FGFR, survivin, and TNF-a
CC       (PubMed:31908094). {ECO:0000269|PubMed:31908094}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAU31929.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; CH476604; EAU31929.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001216288.1; XM_001216288.1.
DR   AlphaFoldDB; Q0CF67; -.
DR   EnsemblFungi; EAU31929; EAU31929; ATEG_07667.
DR   GeneID; 4322612; -.
DR   eggNOG; ENOG502SD7F; Eukaryota.
DR   HOGENOM; CLU_006524_7_1_1; -.
DR   OrthoDB; 895136at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   SUPFAM; SSF57701; SSF57701; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..593
FT                   /note="Transcription factor ATEG_07667"
FT                   /id="PRO_0000450093"
FT   DNA_BIND        18..47
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
SQ   SEQUENCE   593 AA;  67315 MW;  70AB55850238E0C8 CRC64;
     MERSKSALAH SSSYGQACTQ CYKAKCRCVR TPSGDTCERC IRLKKRCEPS ESVRRRNAQS
     TQTAKVSDRR IARLEDKMES LLSAMQSFIG TTASSASSAN VIQPQQLNCD GLPSSTSYSN
     SSLVTPASST LGFNEGQTFA PDSLTTVSPN SNQNAIFLSQ PYVPSTPAPS PNHADERLHF
     FRTRMLPSFP FLDLTPDMTS WYLRQNRPFL FQAIHTVTTF STQERLTQVE ELKRLIFTSA
     LLKVQSNIDL LLGLLTYLAW STDPFLGRAD LVSRLMMLAI SLVISSHLGR QDALRWTPQM
     EEALRVLTIS EACPSDRLFV AQVRLQLLKQ RSDEARQQDE ARTGTAPTAA SAPRLLYLKT
     LRRELQELRA SFPPDLHQID VLNTHAQYVE LYINQLAYSI SQDSLPLDLA GRMGTGSQLG
     FQRLECLWQS VENIKSWLDN FYKIPCSKLV GQPFHFWSQM ILTITLLKYL TTLKDPDWDC
     QAVRNTVHLI STMDCMLQKL DLSSKEPELQ CNDHLLKFLS KLLSRCRVWA EARWNIASQM
     QDGDTRQCRS ANPDTNSHNY YIPDLDQMVF MQSMDLGDDQ WFENVLGMPT MFY