AZR1_YEAST
ID AZR1_YEAST Reviewed; 613 AA.
AC P50080; D6VV06;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Azole resistance protein 1;
GN Name=AZR1; OrderedLocusNames=YGR224W; ORFNames=G8537;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8701610;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<385::aid-yea910>3.0.co;2-g;
RA van der Aart Q.J.M., Kleine K., Steensma H.Y.;
RT "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1
RT region from the right arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 12:385-390(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11113970;
RX DOI=10.1002/1097-0061(200012)16:16<1469::aid-yea640>3.0.co;2-a;
RA Tenreiro S., Rosa P.C., Viegas C.A., Sa-Correia I.;
RT "Expression of the AZR1 gene (ORF YGR224w), encoding a plasma membrane
RT transporter of the major facilitator superfamily, is required for
RT adaptation to acetic acid and resistance to azoles in Saccharomyces
RT cerevisiae.";
RL Yeast 16:1469-1481(2000).
RN [5]
RP INDUCTION.
RX PubMed=12529331; DOI=10.1074/jbc.m208549200;
RA Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.;
RT "A general strategy to uncover transcription factor properties identifies a
RT new regulator of drug resistance in yeast.";
RL J. Biol. Chem. 278:11427-11432(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Transporter protein required for adaptation to high stress
CC imposed by low-chain organic acids, in particular by acetic acid, and
CC for resistance to azoles, especially to ketoconazole and fluconazole.
CC {ECO:0000269|PubMed:11113970}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11113970};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11113970}.
CC -!- INDUCTION: Transcriptionally regulated by PDR8.
CC {ECO:0000269|PubMed:12529331}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; X87941; CAA61172.1; -; Genomic_DNA.
DR EMBL; Z73009; CAA97252.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08317.1; -; Genomic_DNA.
DR PIR; S57687; S57687.
DR RefSeq; NP_011740.3; NM_001181353.3.
DR AlphaFoldDB; P50080; -.
DR BioGRID; 33477; 83.
DR DIP; DIP-7543N; -.
DR IntAct; P50080; 2.
DR STRING; 4932.YGR224W; -.
DR TCDB; 2.A.1.3.53; the major facilitator superfamily (mfs).
DR CarbonylDB; P50080; -.
DR PaxDb; P50080; -.
DR PRIDE; P50080; -.
DR EnsemblFungi; YGR224W_mRNA; YGR224W; YGR224W.
DR GeneID; 853139; -.
DR KEGG; sce:YGR224W; -.
DR SGD; S000003456; AZR1.
DR VEuPathDB; FungiDB:YGR224W; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176547; -.
DR HOGENOM; CLU_000960_22_1_1; -.
DR InParanoid; P50080; -.
DR OMA; HLTWRWA; -.
DR BioCyc; YEAST:G3O-30905-MON; -.
DR PRO; PR:P50080; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P50080; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:1901474; F:azole transmembrane transporter activity; IMP:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045117; P:azole transmembrane transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 2.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..613
FT /note="Azole resistance protein 1"
FT /id="PRO_0000173426"
FT TOPO_DOM 1..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..102
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..134
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..298
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..375
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..443
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..472
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 494..581
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 582..602
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 603..613
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 613 AA; 67169 MW; CF65C211E81D3FFF CRC64;
MKGEPKTYSM SDLSYYGEKA QQQNEKQQKQ YVVRRNSTQS TSKQNVSVVL EDNASESNEL
PKGFILYASL IALALSLFLA ALDIMIVSTI IEEVAKQFGS YSEIGWLFTG YSLPNALLAL
IWGRIATPIG FKETMLFAIV IFEIGSLISA LANSMSMLIG GRVIAGVGGC GIQSLSFVIG
STLVEESQRG ILIAVLSCSF AIASVVGPFL GGVFTSSVTW RWCFYVNLPI GGLAFFLFLF
FYNPGLSTFQ ETMDNIRKFP SQFIEIVRNV AYHLLKIKGF SKLNGWRKPF MELIFMYDII
EFVFCSAGFT CILLAFTFGG NRYAWNSASI IILFIIGIVL VVLAGIYDFL VFPKFNIVKA
TPHYQPLMSW TNIKKPGIFT VNIALFLTCA GYISQFTYIV QYFQLIYNDS AWRAAVHLVA
CIISTVVTAI LCGAITDKTR QIKPIIVISS IFGVVGAGIL TLLNNNANNS AHIGLLILPG
VAFGGLAQSS MLASQIQLDK KSPTFRSDFV SITTFNTFCK NLGQALGGVI SNTVFSAAAI
KKLTKANIQL PDGTTVDNLV IYRQTNFDGS HSKLGNIISE SLTDVFYMAL GFYALSLIFA
VFASNKKVTA SLR
