ID   AZUP_METEA              Reviewed;         148 AA.
AC   P04171; C5AWF0;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Pseudoazurin;
DE   Flags: Precursor;
GN   OrderedLocusNames=MexAM1_META1p0857;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
RN   [2]
RP   PROTEIN SEQUENCE OF 26-148.
RX   PubMed=4091802; DOI=10.1042/bj2320451;
RA   Ambler R.P., Tobari J.;
RT   "The primary structures of Pseudomonas AM1 amicyanin and pseudoazurin. Two
RT   new sequence classes of blue copper proteins.";
RL   Biochem. J. 232:451-457(1985).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 26-148.
RX   PubMed=15299445; DOI=10.1107/s0907444994000260;
RA   Inoue T., Kai Y., Harada S., Kasai N., Ohshiro Y., Suzuki S., Kohzuma T.,
RA   Tobari J.;
RT   "Refined crystal structure of pseudoazurin from Methylobacterium extorquens
RT   AM1 at 1.5-A resolution.";
RL   Acta Crystallogr. D 50:317-328(1994).
CC   -!- COFACTOR:
CC       Name=Cu cation; Xref=ChEBI:CHEBI:23378;
CC       Note=Binds 1 copper ion per subunit.;
CC   -!- SUBCELLULAR LOCATION: Periplasm.
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DR   EMBL; CP001510; ACS38778.1; -; Genomic_DNA.
DR   PIR; A00293; CUPSZM.
DR   RefSeq; WP_012752226.1; NC_012808.1.
DR   PDB; 1PMY; X-ray; 1.50 A; A=26-148.
DR   PDBsum; 1PMY; -.
DR   AlphaFoldDB; P04171; -.
DR   SMR; P04171; -.
DR   STRING; 272630.MexAM1_META1p0857; -.
DR   EnsemblBacteria; ACS38778; ACS38778; MexAM1_META1p0857.
DR   KEGG; mea:Mex_1p0857; -.
DR   eggNOG; COG3794; Bacteria.
DR   HOGENOM; CLU_124330_0_0_5; -.
DR   OMA; GFKCAPH; -.
DR   OrthoDB; 1654242at2; -.
DR   EvolutionaryTrace; P04171; -.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd04218; Pseudoazurin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR002386; Amicyanin/Pseudoazurin.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR001235; Copper_blue_Plastocyanin.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR012745; Pseudoazurin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   PRINTS; PR00155; AMICYANIN.
DR   PRINTS; PR00156; COPPERBLUE.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR02375; pseudoazurin; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Copper; Direct protein sequencing; Electron transport;
KW   Metal-binding; Periplasm; Signal; Transport.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:4091802"
FT   CHAIN           26..148
FT                   /note="Pseudoazurin"
FT                   /id="PRO_0000085539"
FT   DOMAIN          30..118
FT                   /note="Plastocyanin-like"
FT   BINDING         65
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         103
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         106
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         111
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   STRAND          27..36
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          39..50
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          55..59
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          61..64
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          89..92
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          97..102
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   TURN            105..110
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   STRAND          112..119
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   HELIX           124..128
FT                   /evidence="ECO:0007829|PDB:1PMY"
FT   HELIX           134..145
FT                   /evidence="ECO:0007829|PDB:1PMY"
SQ   SEQUENCE   148 AA;  15861 MW;  8E2F4F2ACE293B86 CRC64;
     MMIFRALIAA ATLAIAIATT LPAAADEVAV KMLNSGPGGM MVFDPALVRL KPGDSIKFLP
     TDKGHNVETI KGMAPDGADY VKTTVGQEAV VKFDKEGVYG FKCAPHYMMG MVALVVVGDK
     RDNLEAAKSV QHNKLTQKRL DPLFAQIQ