ID   AZUR2_METJ              Reviewed;         129 AA.
AC   P12335;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Azurin iso-2;
OS   Methylomonas sp. (strain J).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylomonas.
OX   NCBI_TaxID=32038;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=2505762; DOI=10.1042/bj2610495;
RA   Ambler R.P., Tobari J.;
RT   "Two distinct azurins function in the electron-transport chain of the
RT   obligate methylotroph Methylomonas J.";
RL   Biochem. J. 261:495-499(1989).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
RX   PubMed=10631606; DOI=10.1007/pl00010653;
RA   Suzuki S., Nakamura N., Yamaguchi K., Kataoka K., Inoue T., Nishio N.,
RA   Kai Y., Tobari J.;
RT   "Spectroscopic and electrochemical properties of two azurins (Az-iso1 and
RT   Az-iso2) from the obligate methylotroph Methylomonas sp. strain J and the
RT   structure of novel Az-iso2.";
RL   J. Biol. Inorg. Chem. 4:749-758(1999).
CC   -!- FUNCTION: This methylothroph organism uses azurin in the oxidation of
CC       methylamine. Iso-2 is probably the acceptor of electrons from
CC       methylamine dehydrogenase.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- INDUCTION: Produced during growth on methylamine.
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DR   PIR; S05247; S05247.
DR   PDB; 1CUO; X-ray; 1.60 A; A=1-129.
DR   PDB; 1UAT; X-ray; 1.90 A; A=1-129.
DR   PDBsum; 1CUO; -.
DR   PDBsum; 1UAT; -.
DR   AlphaFoldDB; P12335; -.
DR   SMR; P12335; -.
DR   EvolutionaryTrace; P12335; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR014068; Azurin.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR008972; Cupredoxin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR02695; azurin; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Copper; Direct protein sequencing; Disulfide bond;
KW   Electron transport; Metal-binding; Periplasm; Transport.
FT   CHAIN           1..129
FT                   /note="Azurin iso-2"
FT                   /id="PRO_0000085541"
FT   DOMAIN          1..129
FT                   /note="Plastocyanin-like"
FT   BINDING         46
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         112
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         117
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   BINDING         121
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT   DISULFID        3..26
FT                   /evidence="ECO:0000250"
FT   STRAND          4..9
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          11..13
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          18..23
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          27..35
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          37..39
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   HELIX           41..44
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          49..52
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   HELIX           53..55
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   HELIX           56..64
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          81..83
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          92..98
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   HELIX           99..101
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          108..111
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   TURN            118..120
FT                   /evidence="ECO:0007829|PDB:1CUO"
FT   STRAND          121..128
FT                   /evidence="ECO:0007829|PDB:1CUO"
SQ   SEQUENCE   129 AA;  13805 MW;  872C6E5B09078BED CRC64;
     ASCETTVTSG DTMTYSTRSI SVPASCAEFT VNFEHKGHMP KTGMGHNWVL AKSADVGDVA
     KEGAHAGADN NFVTPGDKRV IAFTPIIGGG EKTSVKFKVS ALSKDEAYTY FCSYPGHFSM
     MRGTLKLEE