ID   AZUR_BORBR              Reviewed;         150 AA.
AC   P0A321; P00278;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Azurin;
DE   Flags: Precursor;
GN   OrderedLocusNames=BB3856;
OS   Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
OS   (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-588 / NCTC 13252 / RB50;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA   Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA   Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA   Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA   Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA   Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-150.
RC   STRAIN=ATCC 4617 / NCIB 9935 / NCTC 8344 / NRRL B-140;
RA   Ambler R.P.;
RT   "Species differences in the amino-acid sequences of bacterial proteins.";
RL   (In) Hawkes J.G. (eds.);
RL   Chemotaxonomy and serotaxonomy, pp.57-64, Academic Press, London (1968).
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE35830.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BX640448; CAE35830.1; ALT_INIT; Genomic_DNA.
DR   PIR; A00284; AZBR.
DR   AlphaFoldDB; P0A321; -.
DR   SMR; P0A321; -.
DR   STRING; 257310.BB3856; -.
DR   EnsemblBacteria; CAE35830; CAE35830; BB3856.
DR   KEGG; bbr:BB3856; -.
DR   eggNOG; COG3241; Bacteria.
DR   HOGENOM; CLU_112845_1_0_4; -.
DR   Proteomes; UP000001027; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd13922; Azurin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR014068; Azurin.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR028871; BlueCu_1_BS.
DR   InterPro; IPR008972; Cupredoxin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR02695; azurin; 1.
DR   PROSITE; PS00196; COPPER_BLUE; 1.
PE   1: Evidence at protein level;
KW   Copper; Direct protein sequencing; Disulfide bond; Electron transport;
KW   Metal-binding; Periplasm; Signal; Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           22..150
FT                   /note="Azurin"
FT                   /id="PRO_0000002859"
FT   DOMAIN          22..150
FT                   /note="Plastocyanin-like"
FT   BINDING         67
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         133
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   DISULFID        24..47
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   150 AA;  15973 MW;  531E739204D936FA CRC64;
     MFKQVLGGMA LMAAFSAPVL AAECSVDIAG TDQMQFDKKA IEVSKSCKQF TVNLKHTGKL
     PRNVMGHNWV LTKTADMQAV EKDGIAAGLD NQYLKAGDTR VLAHTKVLGG GESDSVTFDV
     AKLAAGDDYT FFCSFPGHGA LMKGTLKLVD