ID   AZUR_METFK              Reviewed;         148 AA.
AC   Q50400; Q1H3W1;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Azurin;
DE   Flags: Precursor;
GN   Name=azu; OrderedLocusNames=Mfla_0556;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7635847; DOI=10.1128/jb.177.15.4575-4578.1995;
RA   Gak E.R., Chistoserdov A.Y., Lidstrom M.E.;
RT   "Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus
RT   flagellatum KT.";
RL   J. Bacteriol. 177:4575-4578(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KT / ATCC 51484 / DSM 6875;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable electron acceptor for methylamine dehydrogenase.
CC   -!- PATHWAY: One-carbon metabolism; methylamine degradation.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABE48826.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L37426; AAC41479.1; -; Genomic_DNA.
DR   EMBL; CP000284; ABE48826.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_048811529.1; NC_007947.1.
DR   AlphaFoldDB; Q50400; -.
DR   SMR; Q50400; -.
DR   STRING; 265072.Mfla_0556; -.
DR   EnsemblBacteria; ABE48826; ABE48826; Mfla_0556.
DR   KEGG; mfa:Mfla_0556; -.
DR   eggNOG; COG3241; Bacteria.
DR   HOGENOM; CLU_112845_1_0_4; -.
DR   OrthoDB; 1735306at2; -.
DR   UniPathway; UPA00895; -.
DR   Proteomes; UP000002440; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   CDD; cd13922; Azurin; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR014068; Azurin.
DR   InterPro; IPR000923; BlueCu_1.
DR   InterPro; IPR008972; Cupredoxin.
DR   Pfam; PF00127; Copper-bind; 1.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   TIGRFAMs; TIGR02695; azurin; 1.
PE   3: Inferred from homology;
KW   Copper; Disulfide bond; Electron transport; Metal-binding; Periplasm;
KW   Reference proteome; Signal; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..148
FT                   /note="Azurin"
FT                   /id="PRO_0000002862"
FT   DOMAIN          19..148
FT                   /note="Plastocyanin-like"
FT   BINDING         65
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         131
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         136
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   BINDING         140
FT                   /ligand="Cu cation"
FT                   /ligand_id="ChEBI:CHEBI:23378"
FT                   /evidence="ECO:0000250"
FT   DISULFID        22..45
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   148 AA;  15875 MW;  29602BF462568990 CRC64;
     MRNQLLFALA FIPTIAAAAS NCEVNVSAGD SMAFNTRSID IPKSCKEFTV NFAHTGTASK
     AGMGHNWVLA RSADVNDLAK AGVEAGIDKN FIPPNDPRVL AYTPLVGGGE KTSVTFKPSI
     LKDGESYSFY CSFAFHSFMM RGTVKLVD