ID   ABAMS_PEA               Reviewed;         764 AA.
AC   Q9LRH7;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Mixed-amyrin synthase;
DE            EC=5.4.99.39;
DE            EC=5.4.99.40;
GN   Name=OSCPSM;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=10848960; DOI=10.1046/j.1432-1327.2000.01357.x;
RA   Morita M., Shibuya M., Kushiro T., Masuda K., Ebizuka Y.;
RT   "Molecular cloning and functional expression of triterpene synthases from
RT   pea (Pisum sativum) new alpha-amyrin-producing enzyme is a multifunctional
RT   triterpene synthase.";
RL   Eur. J. Biochem. 267:3453-3460(2000).
CC   -!- FUNCTION: Multifunctional oxidosqualene cyclase producing alpha- and
CC       beta-amyrin and several other minor triterpenes.
CC       {ECO:0000269|PubMed:10848960}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = beta-amyrin; Xref=Rhea:RHEA:31007,
CC         ChEBI:CHEBI:10352, ChEBI:CHEBI:15441; EC=5.4.99.39;
CC         Evidence={ECO:0000269|PubMed:10848960};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = alpha-amyrin; Xref=Rhea:RHEA:31387,
CC         ChEBI:CHEBI:10213, ChEBI:CHEBI:15441; EC=5.4.99.40;
CC         Evidence={ECO:0000269|PubMed:10848960};
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB034803; BAA97559.1; -; mRNA.
DR   AlphaFoldDB; Q9LRH7; -.
DR   SMR; Q9LRH7; -.
DR   PRIDE; Q9LRH7; -.
DR   KEGG; ag:BAA97559; -.
DR   BRENDA; 5.4.99.40; 4872.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0042561; F:alpha-amyrin synthase activity; IDA:UniProtKB.
DR   GO; GO:0042300; F:beta-amyrin synthase activity; IDA:UniProtKB.
DR   GO; GO:0019745; P:pentacyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..764
FT                   /note="Mixed-amyrin synthase"
FT                   /id="PRO_0000413970"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          514..556
FT                   /note="PFTB 2"
FT   REPEAT          591..631
FT                   /note="PFTB 3"
FT   REPEAT          640..681
FT                   /note="PFTB 4"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   764 AA;  88277 MW;  DED4CAE6CB822197 CRC64;
     MWKLKIGDGG KDRNIFSTNN FVGRQTWEFD PDAGTSQEKA QVEAARQHFY DNRFEVKACS
     DLLWRFQILK EKNFKQTIES VKIKDEEEIS EENVAITLRR AVHHLSTLQS NDGHWPALNA
     GPLFYFPPLV FCMYVTGHLD SIFPYEYRKE ILRYIYCHQN EDGGWGLHVE GHSIMFCTVL
     NYICMRILGE GPNGGKEDAC ARARKWIHDH GSVTHVSSWG KIWLSVLGIF DWCASNPMPP
     EFWMLPSFLL KHPAKMLCYC RLVYMPMSYL YGKRFVGPIT PLILMLREEL LTQPYEKVNW
     KKTRHLCAKE DLYYPHPLIQ DLIWDSLYIF VEPLLTHWPF NKLLREKALQ TVMKHIHYED
     ENSRYITIGC VEKVLCILAC WVEDPNGDAF KKHLARLPDY LWVSEDGMTL HSFGSQTWDA
     SLIIQALLAT NLIEDVGPIL TKAHEFIKKS QVRDNPSGDF KSMYRHISKG SWTFSDKDHG
     WQVSDCTAES LKCCLLLSML PPEIVGEKME PEMLYDSVNI LLSLQGKKGG LPAWEPSEAV
     EWLELFNPIE FLEEIVVERE YVECTSSAIQ ALVLFKKLYP EHRKKEVENF IANAVRFLEY
     KQTSDGSWYG NWGICFTYGS WFALNGLVAA GKTYDNCAAI RKGVEFLLTT QREDGGWGES
     HLSSSKKIYV PLERSQSNIV QTSWAIMGLI HAGQMERDPT PLHRAVKLII NFQQEEGDWP
     QQELTGVFMK NCMLQYAMYR DIFPTWALAE YRRRILLASP AVAI