ID   B1DYC_RANDY             Reviewed;          20 AA.
AC   P0C5W8;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Brevinin-1DYc;
OS   Rana dybowskii (Dybovsky's frog) (Korean brown frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=71582;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=17688900; DOI=10.1016/j.toxicon.2007.06.023;
RA   Conlon J.M., Kolodziejek J., Nowotny N., Leprince J., Vaudry H., Coquet L.,
RA   Jouenne T., Iwamuro S.;
RT   "Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families
RT   isolated from the skin of the Japanese brown frog, Rana dybowskii.";
RL   Toxicon 50:746-756(2007).
CC   -!- FUNCTION: Antimicrobial peptide. Has low activity against the Gram-
CC       positive bacterium S.aureus and the Gram-negative bacterium E.coli
CC       (MIC<15 uM). Has a strong hemolytic activity.
CC       {ECO:0000269|PubMed:17688900}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=2274.3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17688900};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0C5W8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012520; Antimicrobial_frog_1.
DR   Pfam; PF08018; Antimicrobial_1; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Hemolysis; Secreted.
FT   PEPTIDE         1..20
FT                   /note="Brevinin-1DYc"
FT                   /id="PRO_0000311596"
FT   DISULFID        14..20
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   20 AA;  2278 MW;  69D6856FA52C7595 CRC64;
     FLPLLLAGLP KLLCLFFKKC