ID   B1HN1_ODOHA             Reviewed;          71 AA.
AC   E7EKC4;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Brevinin-1HN1 {ECO:0000303|PubMed:22450466};
DE   Flags: Precursor;
OS   Odorrana hainanensis (Odor frog) (Rana hainanensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Odorrana.
OX   NCBI_TaxID=431935;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ADV36124.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-71, SYNTHESIS OF 48-71,
RP   FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Skin {ECO:0000269|PubMed:22450466}, and
RC   Skin secretion {ECO:0000269|PubMed:22450466};
RX   PubMed=22450466; DOI=10.1016/j.peptides.2012.03.007;
RA   Wang H., Yu Z., Hu Y., Li F., Liu L., Zheng H., Meng H., Yang S., Yang X.,
RA   Liu J.;
RT   "Novel antimicrobial peptides isolated from the skin secretions of Hainan
RT   odorous frog, Odorrana hainanensis.";
RL   Peptides 35:285-290(2012).
CC   -!- FUNCTION: Has antimicrobial activity against Gram-positive bacteria and
CC       fungi but has weak or no activity against a range of Gram-negative
CC       bacteria except P.faecalis. Active against the Gram-positive bacteria
CC       E.faecium 091299 (MIC=19 uM), E.faecalis 981 (MIC=19 uM), S.aureus ATCC
CC       25923 (MIC=1.2 uM), S.carnosus KHS (MIC=4.8 uM), B.licheniformis X39
CC       (MIC=2.4 uM) and R.rhodochrous X15 (MIC=1.2 uM). Active against the
CC       Gram-negative bacterium P.faecalis X29 (MIC=4.8 uM), is virtually
CC       inactive against E.coli ATCC 25922 (MIC=150 uM) and inactive against
CC       P.aeruginosa and S.typhi. Has antifungal activity against C.albicans
CC       ATCC 2002 (MIC=2.4 uM) and is also active against the slime mold 090223
CC       (MIC=1.2 uM). Has low hemolytic activity against human erythrocytes
CC       (LC(50)=75 uM). {ECO:0000269|PubMed:22450466}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22450466}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:22450466}.
CC   -!- MASS SPECTROMETRY: Mass=2664.40; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22450466};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000255}.
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DR   EMBL; HQ735101; ADV36124.1; -; mRNA.
DR   AlphaFoldDB; E7EKC4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012520; Antimicrobial_frog_1.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF08018; Antimicrobial_1; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Cytolysis; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Hemolysis; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..45
FT                   /evidence="ECO:0000255, ECO:0000269|PubMed:22450466"
FT                   /id="PRO_0000423521"
FT   PEPTIDE         48..71
FT                   /note="Brevinin-1HN1"
FT                   /evidence="ECO:0000269|PubMed:22450466"
FT                   /id="PRO_0000423522"
FT   DISULFID        65..71
FT                   /evidence="ECO:0000269|PubMed:22450466"
SQ   SEQUENCE   71 AA;  8276 MW;  A799D047166148F1 CRC64;
     MFTSKKPLLL LFFLGTINLS LCEQERDADE EERRDDPDER DVEVEKRFLP LIASLAANFV
     PKIFCKITKK C