ID   ABAN1_LIMMU             Reviewed;          11 AA.
AC   C0HL60;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=Antibacterial peptide AN1 {ECO:0000305|PubMed:29064056};
DE   AltName: Full=Bacteriocin AN1 {ECO:0000303|PubMed:29064056};
DE   Flags: Fragment;
OS   Limosilactobacillus mucosae (Lactobacillus mucosae).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Limosilactobacillus.
OX   NCBI_TaxID=97478;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   STRAIN=AN1 {ECO:0000303|PubMed:29064056};
RX   PubMed=29064056; DOI=10.1007/s12602-017-9341-3;
RA   Repally A., Perumal V., Dasari A., Palanichamy E., Venkatesan A.;
RT   "Isolation, Identification of Lactobacillus mucosae AN1 and its
RT   Antilisterial Peptide Purification and Characterization.";
RL   Probiotics Antimicrob. Proteins 10:775-786(2018).
CC   -!- FUNCTION: Has antibacterial activity against L.monocytogenes.
CC       {ECO:0000269|PubMed:29064056}.
CC   -!- ACTIVITY REGULATION: Antimicrobial activity was completely lost after
CC       treatment with proteinase k, or high concentrations of chymotrypsin,
CC       trypsin or pepsin (PubMed:29064056). Also inhibited by isopropanol,
CC       methanol, acetonitrile and chloroform (PubMed:29064056). There was no
CC       significant loss of activity with either lipase, peroxidase, or amylase
CC       (PubMed:29064056). {ECO:0000269|PubMed:29064056}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is about 6. Activity decreases sharply with increasing
CC         alkalinity. {ECO:0000269|PubMed:29064056};
CC       Temperature dependence:
CC         Optimum temperature is 30 degrees Celsius. Active from 30 to 100
CC         degrees Celsius. {ECO:0000269|PubMed:29064056};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29064056}.
CC   -!- MASS SPECTROMETRY: Mass=10668; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:29064056};
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:1900426; P:positive regulation of defense response to bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Secreted.
FT   PEPTIDE         1..11
FT                   /note="Antibacterial peptide AN1"
FT                   /id="PRO_0000454660"
FT   NON_TER         11
FT                   /evidence="ECO:0000303|PubMed:29064056"
SQ   SEQUENCE   11 AA;  1248 MW;  48439DA5CB01AAA7 CRC64;
     LVALDTEFAN R