ID   B1ITB_RANIT             Reviewed;          20 AA.
AC   C0HL48;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Brevinin-1ITb {ECO:0000303|PubMed:28699258};
OS   Rana italica (Italian stream frog) (Rana graeca italica).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=147302 {ECO:0000303|PubMed:28699258};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   OXIDATION AT MET-8, AND DISULFIDE BOND.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:28699258};
RX   PubMed=28699258; DOI=10.1002/psc.3025;
RA   Conlon J.M., Musale V., Attoub S., Mangoni M.L., Leprince J., Coquet L.,
RA   Jouenne T., Abdel-Wahab Y.H.A., Flatt P.R., Rinaldi A.C.;
RT   "Cytotoxic peptides with insulin-releasing activities from skin secretions
RT   of the Italian stream frog Rana italica (Ranidae).";
RL   J. Pept. Sci. 23:769-776(2017).
CC   -!- FUNCTION: Antimicrobial peptide. {ECO:0000250|UniProtKB:C0HL47}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28699258}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:28699258}.
CC   -!- MASS SPECTROMETRY: Mass=2227.2; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28699258};
CC   -!- MASS SPECTROMETRY: Mass=2243.2; Method=MALDI; Note=Oxidized.;
CC       Evidence={ECO:0000269|PubMed:28699258};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HL48; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0098542; P:defense response to other organism; IEA:InterPro.
DR   InterPro; IPR012520; Antimicrobial_frog_1.
DR   Pfam; PF08018; Antimicrobial_1; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Oxidation; Secreted.
FT   PEPTIDE         1..20
FT                   /note="Brevinin-1ITb"
FT                   /evidence="ECO:0000269|PubMed:28699258"
FT                   /id="PRO_0000442263"
FT   MOD_RES         8
FT                   /note="Methionine sulfoxide; partial"
FT                   /evidence="ECO:0000269|PubMed:28699258"
FT   DISULFID        14..20
FT                   /evidence="ECO:0000269|PubMed:28699258"
SQ   SEQUENCE   20 AA;  2230 MW;  25BBF5BA8DC9EB92 CRC64;
     IVPFLLGMVP KLICLITKKC