ID   ABAQ_ACIBT              Reviewed;         434 AA.
AC   P0DPR4;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-DEC-2018, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Quinolone resistance transporter {ECO:0000303|PubMed:29941648};
GN   Name=abaQ {ECO:0000303|PubMed:29941648}; ORFNames=AUO97_17040;
OS   Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC
OS   KC755 / 5377).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX   NCBI_TaxID=400667;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377;
RA   Singh M., Fernando D., Kumar A.;
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377;
RX   PubMed=28507065; DOI=10.1128/iai.00327-17;
RA   Perez-Varela M., Corral J., Vallejo J.A., Rumbo-Feal S., Bou G., Aranda J.,
RA   Barbe J.;
RT   "Mutations in the beta-subunit of the RNA polymerase impair the surface-
RT   associated motility and virulence of Acinetobacter baumannii.";
RL   Infect. Immun. 85:E00327-E00327(2017).
RN   [3]
RP   FUNCTION IN QUINOLONE RESISTANCE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377;
RX   PubMed=29941648; DOI=10.1128/aac.00906-18;
RA   Perez-Varela M., Corral J., Aranda J., Barbe J.;
RT   "Functional characterization of AbaQ, a novel efflux pump mediating
RT   quinolone resistance in Acinetobacter baumannii.";
RL   Antimicrob. Agents Chemother. 62:E00906-E00906(2018).
CC   -!- FUNCTION: Efflux pump that mediates resistance to quinolone-type
CC       antibiotics. {ECO:0000269|PubMed:29941648}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation of the gene causes a significant
CC       reduction in the surface-associated motility and virulence
CC       (PubMed:28507065). Mutant has 2- to 4-fold higher susceptibility to
CC       novobiocin and trimethoprim, and 8- to 32-fold higher susceptibility to
CC       the quinolone-type antibiotics nalidixic acid, levofloxacin and
CC       ciprofloxacin (PubMed:29941648). Mutation does not affect
CC       susceptibility to beta-lactams, aminoglycosides, macrolides and
CC       polymyxins (PubMed:29941648). {ECO:0000269|PubMed:28507065,
CC       ECO:0000269|PubMed:29941648}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP018664; APP32449.1; -; Genomic_DNA.
DR   RefSeq; WP_000345069.1; NZ_CP053098.1.
DR   AlphaFoldDB; P0DPR4; -.
DR   SMR; P0DPR4; -.
DR   GeneID; 66397048; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..434
FT                   /note="Quinolone resistance transporter"
FT                   /id="PRO_0000445957"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        396..416
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   434 AA;  47931 MW;  5B9002EF88E449D0 CRC64;
     MDFEKDVIRT VTFKLIPALV ILYLVAYIDR AAVGFAHLHM GADVGIGDAA YGLGAGLFFI
     GYFLFEVPSN LLLDKFGARK WFTRILLTWG LITMAMALIQ GPKSFYLLRF LLGVAEAGFF
     PGVLYLITQW YPVRHRGKIM GMFVLSQPIA MMIAGPLAGL LLGMDGIANL HGWQWLFVAV
     GLPAVLLALP TFLWLPDNID KVKWLSIEQK QWLKNELVKD EAEYDQTRHA NPLHALKDKR
     VLLLALYYLP VTLSIYGLNL WLPTIIKQFG GGSDIQIGFL SSIPYIFGII GLLIIPRSTD
     RLNDRYGHLS FLYALGACAM FLSGWLNSPV MQLAALAVVA FCLFSSTAVF WTLPGRFLTG
     ASAAAGIALI NSVGNLGGYV GPFGIGLLKE YTGNMAAGLY FLSIVMLFGL ILTYIVYAKL
     ERQKTQTVNI QKPL