RS15_THETH
ID RS15_THETH Reviewed; 89 AA.
AC P80378;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=30S ribosomal protein S15 {ECO:0000255|HAMAP-Rule:MF_01343};
GN Name=rpsO {ECO:0000255|HAMAP-Rule:MF_01343};
GN Synonyms=rps15 {ECO:0000255|HAMAP-Rule:MF_01343};
OS Thermus thermophilus.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=274;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=VK1;
RX PubMed=9208917; DOI=10.1111/j.1432-1033.1997.00291.x;
RA Serganov A., Rak A., Garber M.B., Reinbolt J., Ehresmann B., Ehresmann C.,
RA Grunberg-Manago M., Portier C.;
RT "Ribosomal protein S15 from Thermus thermophilus -- cloning, sequencing,
RT overexpression of the gene and RNA-binding properties of the protein.";
RL Eur. J. Biochem. 246:291-300(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=VK1;
RA Serganov A.A., Garber M.B., Portier C.;
RT "Polynucleotide phosphorylase from Thermus thermophilus: cloning,
RT sequencing and expression of the gene and biochemical properties of the
RT enzyme.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP STRUCTURE BY NMR.
RX PubMed=8989316; DOI=10.1038/nsb0197-20;
RA Berglund H., Rak A., Serganov A., Garber M.B., Haerd T.;
RT "Solution structure of the ribosomal RNA binding protein S15 from Thermus
RT thermophilus.";
RL Nat. Struct. Biol. 4:20-23(1997).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RC STRAIN=VK1;
RX PubMed=11071808; DOI=10.1006/jmbi.2000.4204;
RA Ennifar E., Nikulin A., Tishchenko S., Serganov A., Nevskaya N.,
RA Garber M.B., Ehresmann B., Ehresmann C., Nikonov S., Dumas P.;
RT "The crystal structure of UUCG tetraloop.";
RL J. Mol. Biol. 304:35-42(2000).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF S15 AND RRNA.
RC STRAIN=VK1;
RX PubMed=10742169; DOI=10.1038/74028;
RA Nikulin A., Serganov A., Ennifar E., Tishchenko S., Nevskaya N.,
RA Shepard W., Portier C., Garber M.B., Ehresmann B., Ehresmann C.,
RA Nikonov S., Dumas P.;
RT "Crystal structure of the S15-rRNA complex.";
RL Nat. Struct. Biol. 7:273-277(2000).
CC -!- FUNCTION: One of the primary rRNA binding proteins, it binds directly
CC to 16S rRNA where it helps nucleate assembly of the platform of the 30S
CC subunit by binding and bridging several RNA helices of the 16S rRNA.
CC -!- FUNCTION: Forms an intersubunit bridge (bridge B4) with the 23S rRNA of
CC the 50S subunit in the ribosome. {ECO:0000255|HAMAP-Rule:MF_01343}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Forms a bridge to the 50S
CC subunit in the 70S ribosome, contacting the 23S rRNA.
CC {ECO:0000255|HAMAP-Rule:MF_01343}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS15 family.
CC {ECO:0000255|HAMAP-Rule:MF_01343}.
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DR EMBL; Z37726; CAA85782.1; -; Genomic_DNA.
DR EMBL; Z84207; CAB06340.1; -; Genomic_DNA.
DR RefSeq; WP_008632621.1; NZ_VHHQ01000002.1.
DR PDB; 1AB3; NMR; -; A=2-89.
DR PDB; 1DK1; X-ray; 2.80 A; A=2-87.
DR PDB; 1F7Y; X-ray; 2.80 A; A=1-89.
DR PDB; 1KUQ; X-ray; 2.84 A; A=1-87.
DR PDB; 2FKX; NMR; -; A=2-89.
DR PDB; 4V49; X-ray; 8.70 A; O=2-89.
DR PDB; 4V8X; X-ray; 3.35 A; AO/CO=1-89.
DR PDBsum; 1AB3; -.
DR PDBsum; 1DK1; -.
DR PDBsum; 1F7Y; -.
DR PDBsum; 1KUQ; -.
DR PDBsum; 2FKX; -.
DR PDBsum; 4V49; -.
DR PDBsum; 4V8X; -.
DR AlphaFoldDB; P80378; -.
DR SMR; P80378; -.
DR DIP; DIP-6174N; -.
DR GeneID; 3169463; -.
DR OMA; FKTHVKD; -.
DR EvolutionaryTrace; P80378; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00353; Ribosomal_S15p_S13e; 1.
DR HAMAP; MF_01343_B; Ribosomal_S15_B; 1.
DR InterPro; IPR000589; Ribosomal_S15.
DR InterPro; IPR005290; Ribosomal_S15_bac-type.
DR InterPro; IPR009068; S15_NS1_RNA-bd.
DR PANTHER; PTHR23321; PTHR23321; 1.
DR Pfam; PF00312; Ribosomal_S15; 1.
DR SMART; SM01387; Ribosomal_S15; 1.
DR SUPFAM; SSF47060; SSF47060; 1.
DR TIGRFAMs; TIGR00952; S15_bact; 1.
DR PROSITE; PS00362; RIBOSOMAL_S15; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..89
FT /note="30S ribosomal protein S15"
FT /id="PRO_0000115572"
FT HELIX 5..15
FT /evidence="ECO:0007829|PDB:1DK1"
FT TURN 19..22
FT /evidence="ECO:0007829|PDB:1AB3"
FT HELIX 26..45
FT /evidence="ECO:0007829|PDB:1DK1"
FT TURN 46..48
FT /evidence="ECO:0007829|PDB:1DK1"
FT HELIX 51..73
FT /evidence="ECO:0007829|PDB:1DK1"
FT HELIX 75..81
FT /evidence="ECO:0007829|PDB:1DK1"
FT TURN 82..86
FT /evidence="ECO:0007829|PDB:1DK1"
SQ SEQUENCE 89 AA; 10554 MW; 9DB7E1C533DBF340 CRC64;
MPITKEEKQK VIQEFARFPG DTGSTEVQVA LLTLRINRLS EHLKVHKKDH HSHRGLLMMV
GQRRRLLRYL QREDPERYRA LIEKLGIRG
