ID   B1_USTMA                Reviewed;         410 AA.
AC   P22015; A0A0D1E8M0; Q4PH36;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Mating-type locus allele B1 protein;
GN   ORFNames=UMAG_12052;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=1967554; DOI=10.1016/0092-8674(90)90744-y;
RA   Schulz B., Banuett F., Dahl M., Schlesinger R., Schaefer W., Martin T.,
RA   Herskowitz I., Kahmann R.;
RT   "The b alleles of U. maydis, whose combinations program pathogenic
RT   development, code for polypeptides containing a homeodomain-related
RT   motif.";
RL   Cell 60:295-306(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=2227416; DOI=10.1101/gad.4.8.1384;
RA   Kronstad J.W., Leong S.A.;
RT   "The b mating-type locus of Ustilago maydis contains variable and constant
RT   regions.";
RL   Genes Dev. 4:1384-1395(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The B locus has at least 25 alleles, and any combination of
CC       two different B alleles yields a multimeric regulatory protein, that
CC       activates genes responsible for the pathogenicity and for the sexual
CC       development of the fungus within the corn plant.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=KIS72159.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; M58553; AAA63553.1; -; Genomic_DNA.
DR   EMBL; X61305; CAA43602.1; -; Genomic_DNA.
DR   EMBL; CM003140; KIS72159.1; ALT_SEQ; Genomic_DNA.
DR   PIR; A32696; A32696.
DR   PIR; A36671; A36671.
DR   RefSeq; XP_011386823.1; XM_011388521.1.
DR   AlphaFoldDB; P22015; -.
DR   SMR; P22015; -.
DR   EnsemblFungi; KIS72159; KIS72159; UMAG_12052.
DR   GeneID; 23567838; -.
DR   KEGG; uma:UMAG_12052; -.
DR   eggNOG; ENOG502RSBJ; Eukaryota.
DR   HOGENOM; CLU_671208_0_0_1; -.
DR   InParanoid; P22015; -.
DR   OMA; LWFINAR; -.
DR   OrthoDB; 1288080at2759; -.
DR   Proteomes; UP000000561; Chromosome 1.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR008422; Homeobox_KN_domain.
DR   InterPro; IPR008888; Ustilago_mating.
DR   Pfam; PF05920; Homeobox_KN; 1.
DR   Pfam; PF05722; Ustilago_mating; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..410
FT                   /note="Mating-type locus allele B1 protein"
FT                   /id="PRO_0000049401"
FT   DNA_BIND        107..184
FT                   /note="Homeobox; TALE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..110
FT                   /note="Variable domain between B alleles"
FT   REGION          111..410
FT                   /note="Highly conserved between B alleles"
FT   REGION          202..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          278..335
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          333..410
FT                   /note="Not essential for B1 function"
FT   REGION          374..395
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           276..308
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        202..229
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        309..335
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        168..169
FT                   /note="QL -> HV (in Ref. 1; AAA63553)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        218
FT                   /note="S -> Y (in Ref. 1; AAA63553)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        292
FT                   /note="K -> N (in Ref. 1; AAA63553)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   410 AA;  46197 MW;  AF7AA3EC852F2985 CRC64;
     MSSDPNFSLI SFLECLNEIE HEFLRDKGEN YPVLVRKLRE LQQKIPNDIA NLPRDPETIQ
     QIHQTTHRIR AVAQAFIRFD QKFVSLCSEV VHGTSKVMQE FNVVSPDVGC RNLSEDLPAY
     HMRKHFLLTL DNPYPTQEEK ETLVRLTNES TARVGQSSVN RPPLEVHQLT LWFINARRRS
     GWSHILKKFA REDRSRMKHL VRAKLSSSNQ STPPSSTSDS LSNNLDDVLS DNLGRPLTPV
     DKQQFEDDWA SMISWIKYGV KEKVGDWVYD LCAASKKTPK PGMPRPVTTV AKRHPARKTK
     PAAKPKSRTA NPRASTTPSI DSTLDSSKLE STPELSMCST ADTSFSTFGS SLSMSHYNPF
     QDGNDILQSP TVKARGNRKV KALPKRAGKQ QPDEVDNGKI PFLCLSVAFV