ID   B2DYE_RANDY             Reviewed;          37 AA.
AC   P0C5X5;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Brevinin-2DYe;
DE   AltName: Full=Brevinin-2CDYa;
OS   Rana dybowskii (Dybovsky's frog) (Korean brown frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=71582;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=17688900; DOI=10.1016/j.toxicon.2007.06.023;
RA   Conlon J.M., Kolodziejek J., Nowotny N., Leprince J., Vaudry H., Coquet L.,
RA   Jouenne T., Iwamuro S.;
RT   "Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families
RT   isolated from the skin of the Japanese brown frog, Rana dybowskii.";
RL   Toxicon 50:746-756(2007).
RN   [2]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Skin secretion;
RX   PubMed=19539775; DOI=10.1016/j.cbpb.2009.05.015;
RA   Jin L.-L., Li Q., Song S.-S., Feng K., Zhang D.-B., Wang Q.-Y., Chen Y.-H.;
RT   "Characterization of antimicrobial peptides isolated from the skin of the
RT   Chinese frog, Rana dybowskii.";
RL   Comp. Biochem. Physiol. 154B:174-178(2009).
CC   -!- FUNCTION: Antimicrobial peptide. Active against the Gram-positive
CC       bacterium S.aureus (MIC=15 uM) and the Gram-negative bacterium E.coli
CC       (MIC=30 uM). {ECO:0000269|PubMed:17688900}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19539775}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:19539775}.
CC   -!- MASS SPECTROMETRY: Mass=3671.7; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17688900};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C5X5; -.
DR   SMR; P0C5X5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..37
FT                   /note="Brevinin-2DYe"
FT                   /id="PRO_0000311603"
FT   DISULFID        31..37
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   37 AA;  3675 MW;  3D6C8EEFF95B8AF7 CRC64;
     GLFSVVTGVL KAVGKNVAKN VGGSLLEQLK CKISGGC