B2L14_HUMAN
ID B2L14_HUMAN Reviewed; 327 AA.
AC Q9BZR8; A8KAD0; Q96QR5; Q9BZR7;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Apoptosis facilitator Bcl-2-like protein 14;
DE Short=Bcl2-L-14;
DE AltName: Full=Apoptosis regulator Bcl-G;
GN Name=BCL2L14; Synonyms=BCLG;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP SUBCELLULAR LOCATION.
RX PubMed=11054413; DOI=10.1074/jbc.m005889200;
RA Guo B., Godzik A., Reed J.C.;
RT "Bcl-G, a novel pro-apoptotic member of the Bcl-2 family.";
RL J. Biol. Chem. 276:2780-2785(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RX PubMed=15284860; DOI=10.1038/sj.leu.2403441;
RA Montpetit A., Larose J., Boily G., Langlois S., Trudel N., Sinnett D.;
RT "Mutational and expression analysis of the chromosome 12p candidate tumor
RT suppressor genes in pre-B acute lymphoblastic leukemia.";
RL Leukemia 18:1499-1504(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION BY MELK.
RX PubMed=17280616; DOI=10.1186/bcr1650;
RA Lin M.L., Park J.H., Nishidate T., Nakamura Y., Katagiri T.;
RT "Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary
RT carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of
RT the Bcl-2 family.";
RL Breast Cancer Res. 9:R17-R17(2007).
CC -!- FUNCTION: Plays a role in apoptosis.
CC -!- INTERACTION:
CC Q9BZR8; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-1385773, EBI-742054;
CC Q9BZR8; Q08380: LGALS3BP; NbExp=2; IntAct=EBI-1385773, EBI-354956;
CC Q9BZR8; Q14680: MELK; NbExp=4; IntAct=EBI-1385773, EBI-1046702;
CC Q9BZR8; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-1385773, EBI-79165;
CC Q9BZR8; Q96N21: TEPSIN; NbExp=3; IntAct=EBI-1385773, EBI-11139477;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11054413}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, cytosol. Note=Diffusely
CC distributed throughout the cytosol.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Endomembrane system.
CC Note=Predominantly localized to cytosolic organelles.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Bcl-Gl, Long;
CC IsoId=Q9BZR8-1; Sequence=Displayed;
CC Name=2; Synonyms=Bcl-Gs, Short;
CC IsoId=Q9BZR8-2; Sequence=VSP_012239, VSP_012240;
CC Name=3; Synonyms=Median;
CC IsoId=Q9BZR8-3; Sequence=VSP_012241, VSP_012242;
CC -!- TISSUE SPECIFICITY: Isoform 1 is widely expressed. Isoform 2 is testis-
CC specific. {ECO:0000269|PubMed:11054413}.
CC -!- PTM: Phosphorylated by MELK, leading to inhibit its pro-apoptotic
CC function. {ECO:0000269|PubMed:17280616}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Bcl-2 family. {ECO:0000305}.
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DR EMBL; AF281254; AAG59793.1; -; mRNA.
DR EMBL; AF281255; AAG59794.1; -; mRNA.
DR EMBL; AY040274; AAK72109.1; -; mRNA.
DR EMBL; AK292995; BAF85684.1; -; mRNA.
DR EMBL; CH471094; EAW96247.1; -; Genomic_DNA.
DR EMBL; BC025778; AAH25778.1; -; mRNA.
DR CCDS; CCDS8645.1; -. [Q9BZR8-1]
DR CCDS; CCDS8646.1; -. [Q9BZR8-2]
DR RefSeq; NP_110393.1; NM_030766.1. [Q9BZR8-2]
DR RefSeq; NP_620048.1; NM_138722.1. [Q9BZR8-1]
DR RefSeq; NP_620049.1; NM_138723.1. [Q9BZR8-1]
DR RefSeq; XP_006719214.1; XM_006719151.3. [Q9BZR8-1]
DR RefSeq; XP_011519150.1; XM_011520848.1. [Q9BZR8-1]
DR RefSeq; XP_011519151.1; XM_011520849.1. [Q9BZR8-1]
DR RefSeq; XP_016875452.1; XM_017019963.1.
DR AlphaFoldDB; Q9BZR8; -.
DR BioGRID; 122658; 122.
DR IntAct; Q9BZR8; 118.
DR MINT; Q9BZR8; -.
DR STRING; 9606.ENSP00000309132; -.
DR iPTMnet; Q9BZR8; -.
DR PhosphoSitePlus; Q9BZR8; -.
DR BioMuta; BCL2L14; -.
DR DMDM; 56748611; -.
DR jPOST; Q9BZR8; -.
DR MassIVE; Q9BZR8; -.
DR PaxDb; Q9BZR8; -.
DR PeptideAtlas; Q9BZR8; -.
DR PRIDE; Q9BZR8; -.
DR ProteomicsDB; 79895; -. [Q9BZR8-1]
DR ProteomicsDB; 79896; -. [Q9BZR8-2]
DR ProteomicsDB; 79897; -. [Q9BZR8-3]
DR Antibodypedia; 11858; 295 antibodies from 35 providers.
DR DNASU; 79370; -.
DR Ensembl; ENST00000266434.8; ENSP00000266434.4; ENSG00000121380.12. [Q9BZR8-2]
DR Ensembl; ENST00000298566.2; ENSP00000298566.1; ENSG00000121380.12. [Q9BZR8-3]
DR Ensembl; ENST00000308721.9; ENSP00000309132.4; ENSG00000121380.12. [Q9BZR8-1]
DR Ensembl; ENST00000396367.5; ENSP00000379653.1; ENSG00000121380.12. [Q9BZR8-1]
DR Ensembl; ENST00000589718.5; ENSP00000467086.1; ENSG00000121380.12. [Q9BZR8-1]
DR Ensembl; ENST00000627413.2; ENSP00000486050.1; ENSG00000281449.2. [Q9BZR8-1]
DR Ensembl; ENST00000628315.1; ENSP00000485980.1; ENSG00000281449.2. [Q9BZR8-3]
DR Ensembl; ENST00000628783.2; ENSP00000487330.1; ENSG00000281449.2. [Q9BZR8-1]
DR Ensembl; ENST00000631251.2; ENSP00000486560.1; ENSG00000281449.2. [Q9BZR8-2]
DR Ensembl; ENST00000631317.2; ENSP00000486796.1; ENSG00000281449.2. [Q9BZR8-1]
DR GeneID; 79370; -.
DR KEGG; hsa:79370; -.
DR MANE-Select; ENST00000308721.9; ENSP00000309132.4; NM_138723.2; NP_620049.1.
DR UCSC; uc001rac.4; human. [Q9BZR8-1]
DR CTD; 79370; -.
DR DisGeNET; 79370; -.
DR GeneCards; BCL2L14; -.
DR HGNC; HGNC:16657; BCL2L14.
DR HPA; ENSG00000121380; Tissue enhanced (intestine, stomach, testis).
DR MIM; 606126; gene.
DR neXtProt; NX_Q9BZR8; -.
DR OpenTargets; ENSG00000121380; -.
DR PharmGKB; PA134870762; -.
DR VEuPathDB; HostDB:ENSG00000121380; -.
DR eggNOG; KOG4728; Eukaryota.
DR GeneTree; ENSGT00940000154318; -.
DR HOGENOM; CLU_073095_0_0_1; -.
DR InParanoid; Q9BZR8; -.
DR OrthoDB; 1288548at2759; -.
DR PhylomeDB; Q9BZR8; -.
DR PathwayCommons; Q9BZR8; -.
DR Reactome; R-HSA-6803205; TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain.
DR SignaLink; Q9BZR8; -.
DR BioGRID-ORCS; 79370; 13 hits in 1072 CRISPR screens.
DR ChiTaRS; BCL2L14; human.
DR GeneWiki; BCL2L14; -.
DR GenomeRNAi; 79370; -.
DR Pharos; Q9BZR8; Tbio.
DR PRO; PR:Q9BZR8; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9BZR8; protein.
DR Bgee; ENSG00000121380; Expressed in rectum and 94 other tissues.
DR ExpressionAtlas; Q9BZR8; baseline and differential.
DR Genevisible; Q9BZR8; HS.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0043229; C:intracellular organelle; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IDA:UniProtKB.
DR GO; GO:2001238; P:positive regulation of extrinsic apoptotic signaling pathway; IDA:UniProtKB.
DR GO; GO:2001236; P:regulation of extrinsic apoptotic signaling pathway; IBA:GO_Central.
DR Gene3D; 1.10.437.10; -; 1.
DR InterPro; IPR036834; Bcl-2-like_sf.
DR InterPro; IPR002475; Bcl2-like.
DR InterPro; IPR033229; BCL2L14.
DR PANTHER; PTHR14965:SF1; PTHR14965:SF1; 1.
DR SUPFAM; SSF56854; SSF56854; 1.
DR PROSITE; PS50062; BCL2_FAMILY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Cytoplasm; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..327
FT /note="Apoptosis facilitator Bcl-2-like protein 14"
FT /id="PRO_0000143075"
FT MOTIF 212..226
FT /note="BH3"
FT MOTIF 308..315
FT /note="BH2"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AYK4"
FT VAR_SEQ 227..276
FT /note="LKKDKALMGHFQDGLSYSVFKTITDQVLMGVDPRGESEVKAQGFKAALVI
FT -> EKEWEESAKTKIKMALLWRSSGGGPRVLSIPEAFMKNSFTLTPPGFSEHN (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:15284860"
FT /id="VSP_012241"
FT VAR_SEQ 227..252
FT /note="LKKDKALMGHFQDGLSYSVFKTITDQ -> DTAFIPIPLVDTSIQGFPQDGL
FT MACI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11054413"
FT /id="VSP_012239"
FT VAR_SEQ 253..327
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11054413"
FT /id="VSP_012240"
FT VAR_SEQ 277..327
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15284860"
FT /id="VSP_012242"
SQ SEQUENCE 327 AA; 36598 MW; 155D43BA91BFB3EA CRC64;
MCSTSGCDLE EIPLDDDDLN TIEFKILAYY TRHHVFKSTP ALFSPKLLRT RSLSQRGLGN
CSANESWTEV SWPCRNSQSS EKAINLGKKK SSWKAFFGVV EKEDSQSTPA KVSAQGQRTL
EYQDSHSQQW SRCLSNVEQC LEHEAVDPKV ISIANRVAEI VYSWPPPQAT QAGGFKSKEI
FVTEGLSFQL QGHVPVASSS KKDEEEQILA KIVELLKYSG DQLERKLKKD KALMGHFQDG
LSYSVFKTIT DQVLMGVDPR GESEVKAQGF KAALVIDVTA KLTAIDNHPM NRVLGFGTKY
LKENFSPWIQ QHGGWEKILG ISHEEVD
