ID   B2RP_LITST              Reviewed;          21 AA.
AC   P0DQK8;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Brevinin-2-related peptide {ECO:0000303|PubMed:15556063};
OS   Lithobates septentrionalis (Mink frog) (Rana septentrionalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=190274;
RN   [1]
RP   PROTEIN SEQUENCE, AMIDATION AT LEU-21, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Skin secretion;
RX   PubMed=15556063; DOI=10.1016/j.cca.2004.08.019;
RA   Bevier C.R., Sonnevend A., Kolodziejek J., Nowotny N., Nielsen P.F.,
RA   Conlon J.M.;
RT   "Purification and characterization of antimicrobial peptides from the skin
RT   secretions of the mink frog (Rana septentrionalis).";
RL   Comp. Biochem. Physiol. 139:31-38(2004).
RN   [2]
RP   FUNCTION.
RX   PubMed=20086159; DOI=10.1128/aac.01448-09;
RA   Wang G., Watson K.M., Peterkofsky A., Buckheit R.W. Jr.;
RT   "Identification of novel human immunodeficiency virus type 1-inhibitory
RT   peptides based on the antimicrobial peptide database.";
RL   Antimicrob. Agents Chemother. 54:1343-1346(2010).
CC   -!- FUNCTION: Antimicrobial peptide with activity against Gram-negative and
CC       Gram-positive bacteria (MIC=13 uM against E.coli, MIC=25 uM against
CC       S.aureus) and fungi (MIC=25 uM against C.albicans) (PubMed:15556063).
CC       Also shows hemolytic activity (HC(50)=50 uM) (PubMed:15556063). In
CC       vitro, shows moderate inhibitory activity against HIV
CC       (PubMed:20086159). {ECO:0000269|PubMed:15556063,
CC       ECO:0000269|PubMed:20086159}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15556063}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:15556063}.
CC   -!- DEVELOPMENTAL STAGE: Is equally expressed in juvenile and adult (male
CC       and female) frogs. {ECO:0000305|PubMed:15556063}.
CC   -!- MASS SPECTROMETRY: Mass=2317.2; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15556063};
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=00599";
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DR   AlphaFoldDB; P0DQK8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0050688; P:regulation of defense response to virus; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Antiviral protein; Cytolysis; Direct protein sequencing; Fungicide;
KW   Hemolysis; Secreted.
FT   PEPTIDE         1..21
FT                   /note="Brevinin-2-related peptide"
FT                   /evidence="ECO:0000269|PubMed:15556063"
FT                   /id="PRO_0000449485"
FT   MOD_RES         21
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:15556063"
SQ   SEQUENCE   21 AA;  2320 MW;  943F9FF51B00EF80 CRC64;
     GIWDTIKSMG KVFAGKILQN L