B2_FHV
ID B2_FHV Reviewed; 106 AA.
AC P68831; P29100; Q89618; Q96632;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 02-JUN-2021, entry version 42.
DE RecName: Full=Protein B2;
GN Name=B2;
OS Flock house virus (FHV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC Nodamuvirales; Nodaviridae; Alphanodavirus.
OX NCBI_TaxID=12287;
OH NCBI_TaxID=50579; Costelytra zealandica.
OH NCBI_TaxID=7137; Galleria mellonella (Greater wax moth).
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4932; Saccharomyces cerevisiae (Baker's yeast).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA Dasgupta R.;
RT "Near identity in the polymerase gene of two serologically distinct
RT nodaviruses.";
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION.
RX PubMed=14745017; DOI=10.1073/pnas.0308308100;
RA Li W.-X., Li H., Lu R., Li F., Dus M., Atkinson P., Brydon E.W.A.,
RA Johnson K.L., Garcia-Sastre A., Ball L.A., Palese P., Ding S.-W.;
RT "Interferon antagonist proteins of influenza and vaccinia viruses are
RT suppressors of RNA silencing.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:1350-1355(2004).
RN [3]
RP FUNCTION, SUBUNIT, AND RNA-BINDING.
RX PubMed=16228003; DOI=10.1038/nsmb1005;
RA Chao J.A., Lee J.H., Chapados B.R., Debler E.W., Schneemann A.,
RA Williamson J.R.;
RT "Dual modes of RNA-silencing suppression by Flock House virus protein B2.";
RL Nat. Struct. Mol. Biol. 12:952-957(2005).
CC -!- FUNCTION: Binds double-strand RNA (dsRNA) with high affinity.
CC Suppresses the host RNA silencing-based antiviral response.
CC {ECO:0000269|PubMed:14745017, ECO:0000269|PubMed:16228003}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16228003}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels early in the viral
CC replication cycle.
CC -!- DOMAIN: The dsRNA-binding region is required for suppression of RNA
CC silencing.
CC -!- MISCELLANEOUS: Encoded on a subgenomic RNA (RNA3) synthesized during
CC replication and which is co-terminal with RNA1.
CC -!- SIMILARITY: Belongs to the nodaviridae B2 protein family.
CC {ECO:0000305}.
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DR EMBL; X77156; CAA54401.1; -; Genomic_RNA.
DR PIR; S41398; S41398.
DR RefSeq; NP_689446.1; NC_004146.1.
DR PDB; 2AZ0; X-ray; 2.60 A; A/B=1-73.
DR PDB; 2AZ2; X-ray; 2.60 A; A/B=1-73.
DR PDB; 2B9Z; NMR; -; A/B=1-72.
DR PDBsum; 2AZ0; -.
DR PDBsum; 2AZ2; -.
DR PDBsum; 2B9Z; -.
DR SMR; P68831; -.
DR DIP; DIP-60497N; -.
DR PRIDE; P68831; -.
DR GeneID; 1724847; -.
DR KEGG; vg:1724847; -.
DR EvolutionaryTrace; P68831; -.
DR Proteomes; UP000203899; Genome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR024377; RNA-bd_B2.
DR InterPro; IPR037209; RNA-bd_B2_sf.
DR Pfam; PF11473; B2; 1.
DR SUPFAM; SSF140506; SSF140506; 1.
PE 1: Evidence at protein level;
KW 3D-structure; RNA-binding; Suppressor of RNA silencing.
FT CHAIN 1..106
FT /note="Protein B2"
FT /id="PRO_0000222454"
FT REGION 1..73
FT /note="RNA-binding"
FT HELIX 4..9
FT /evidence="ECO:0007829|PDB:2AZ0"
FT HELIX 11..20
FT /evidence="ECO:0007829|PDB:2AZ0"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:2AZ0"
FT STRAND 28..30
FT /evidence="ECO:0007829|PDB:2AZ0"
FT HELIX 32..59
FT /evidence="ECO:0007829|PDB:2AZ0"
FT HELIX 63..70
FT /evidence="ECO:0007829|PDB:2AZ0"
SQ SEQUENCE 106 AA; 11646 MW; FA79D28C4AB99CA9 CRC64;
MPSKLALIQE LPDRIQTAVE AAMGMSYQDA PNNVRRDLDN LHACLNKAKL TVSRMVTSLL
EKPSVVAYLE GKAPEEAKPT LEERLRKLEL SHSLPTTGSD PPPAKL
