RS19B_YEAST
ID RS19B_YEAST Reviewed; 144 AA.
AC P07281; D6W0P2;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=40S ribosomal protein S19-B {ECO:0000303|PubMed:9559554};
DE AltName: Full=RP55B;
DE AltName: Full=S16a;
DE AltName: Full=Small ribosomal subunit protein eS19-B {ECO:0000303|PubMed:24524803};
DE AltName: Full=YS16B;
GN Name=RPS19B {ECO:0000303|PubMed:9559554}; Synonyms=RP55B, RPS16AB;
GN OrderedLocusNames=YNL302C; ORFNames=N0422;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6387623; DOI=10.1093/nar/12.19.7345;
RA Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J.,
RA Donovan D.M., Pearson N.J.;
RT "Structure and organization of two linked ribosomal protein genes in
RT yeast.";
RL Nucleic Acids Res. 12:7345-7358(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8553702; DOI=10.1002/yea.320111311;
RA Maurer K.C.T., Urbanus J.H.M., Planta R.J.;
RT "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV
RT carrying a ribosomal protein gene cluster, the genes encoding a plasma
RT membrane protein and a subunit of replication factor C, and a novel
RT putative serine/threonine protein kinase gene.";
RL Yeast 11:1303-1310(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP PROTEIN SEQUENCE OF 2-50.
RX PubMed=6814480; DOI=10.1021/bi00262a005;
RA Otaka E., Higo K., Osawa S.;
RT "Isolation of seventeen proteins and amino-terminal amino acid sequences of
RT eight proteins from cytoplasmic ribosomes of yeast.";
RL Biochemistry 21:4545-4550(1982).
RN [6]
RP NOMENCLATURE, AND SUBUNIT.
RX PubMed=9559554;
RX DOI=10.1002/(sici)1097-0061(19980330)14:5<471::aid-yea241>3.0.co;2-u;
RA Planta R.J., Mager W.H.;
RT "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.";
RL Yeast 14:471-477(1998).
RN [7]
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=10601260; DOI=10.1074/jbc.274.52.37035;
RA Arnold R.J., Polevoda B., Reilly J.P., Sherman F.;
RT "The action of N-terminal acetyltransferases on yeast ribosomal proteins.";
RL J. Biol. Chem. 274:37035-37040(1999).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP FUNCTION IN RIBOSOME SYNTHESIS, AND DISRUPTION PHENOTYPE.
RX PubMed=16159874; DOI=10.1074/jbc.m506916200;
RA Leger-Silvestre I., Caffrey J.M., Dawaliby R., Alvarez-Arias D.A., Gas N.,
RA Bertolone S.J., Gleizes P.E., Ellis S.R.;
RT "Specific role for yeast homologs of the Diamond Blackfan anemia-associated
RT Rps19 protein in ribosome synthesis.";
RL J. Biol. Chem. 280:38177-38185(2005).
RN [11]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=22096102; DOI=10.1126/science.1212642;
RA Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G.,
RA Yusupov M.;
RT "The structure of the eukaryotic ribosome at 3.0 A resolution.";
RL Science 334:1524-1529(2011).
RN [12]
RP NOMENCLATURE.
RX PubMed=24524803; DOI=10.1016/j.sbi.2014.01.002;
RA Ban N., Beckmann R., Cate J.H.D., Dinman J.D., Dragon F., Ellis S.R.,
RA Lafontaine D.L.J., Lindahl L., Liljas A., Lipton J.M., McAlear M.A.,
RA Moore P.B., Noller H.F., Ortega J., Panse V.G., Ramakrishnan V.,
RA Spahn C.M.T., Steitz T.A., Tchorzewski M., Tollervey D., Warren A.J.,
RA Williamson J.R., Wilson D., Yonath A., Yusupov M.;
RT "A new system for naming ribosomal proteins.";
RL Curr. Opin. Struct. Biol. 24:165-169(2014).
CC -!- FUNCTION: Component of the ribosome, a large ribonucleoprotein complex
CC responsible for the synthesis of proteins in the cell. The small
CC ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the
CC encoded message by selecting cognate aminoacyl-transfer RNA (tRNA)
CC molecules. The large subunit (LSU) contains the ribosomal catalytic
CC site termed the peptidyl transferase center (PTC), which catalyzes the
CC formation of peptide bonds, thereby polymerizing the amino acids
CC delivered by tRNAs into a polypeptide chain. The nascent polypeptides
CC leave the ribosome through a tunnel in the LSU and interact with
CC protein factors that function in enzymatic processing, targeting, and
CC the membrane insertion of nascent chains at the exit of the ribosomal
CC tunnel (PubMed:22096102). eS19 is required for proper maturation of the
CC small (40S) ribosomal subunit. Binds to 40S pre-ribosomal particles,
CC probably required after association of NOC4 but before association of
CC ENP1, TSR1 and RIO2 with 20/21S pre-rRNA (PubMed:16159874).
CC {ECO:0000269|PubMed:16159874, ECO:0000305|PubMed:22096102}.
CC -!- FUNCTION: Required for proper maturation of the small (40S) ribosomal
CC subunit. Binds to 40s pre-ribosomal particles, probably required after
CC association of NOC4 but before association of ENP1, TSR1 and RIO2 with
CC 20/21S pre-rRNA. {ECO:0000269|PubMed:16159874}.
CC -!- SUBUNIT: Component of the small ribosomal subunit (SSU). Mature yeast
CC ribosomes consist of a small (40S) and a large (60S) subunit. The 40S
CC small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33
CC different proteins (encoded by 57 genes). The large 60S subunit
CC contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different
CC proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).
CC {ECO:0000269|PubMed:22096102, ECO:0000305|PubMed:9559554}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:22096102}.
CC -!- DISRUPTION PHENOTYPE: Disruption of a single RPS19 gene reduces cell
CC growth; a double disruption is lethal. Depletion experiments show the
CC proteins are required for correct maturation of precursor rRNA to
CC generate the 18S small rRNA. A specific site between the 18S and 5.8S
CC precursors (site A2 in ETS1) is not cleaved in disruption mutants.
CC Partially assembled ribosomes are retained in the nucleolus rather than
CC being exported to the cytoplasm. All effects are exacerbated in the
CC double disruption. Increases association of NOC4 with 20/21S pre-rRNA,
CC decreases association of ENP1, TSR1 and RIO2 with 20/21S pre-rRNA.
CC {ECO:0000269|PubMed:16159874}.
CC -!- MISCELLANEOUS: Present with 3440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- MISCELLANEOUS: There are 2 genes for eS19 in yeast. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eS19 family.
CC {ECO:0000305}.
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DR EMBL; X01100; CAA25575.1; -; Genomic_DNA.
DR EMBL; U23084; AAC49096.1; -; Genomic_DNA.
DR EMBL; Z71578; CAA96220.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10258.1; -; Genomic_DNA.
DR PIR; S60398; S60398.
DR RefSeq; NP_014097.1; NM_001183140.1.
DR AlphaFoldDB; P07281; -.
DR SMR; P07281; -.
DR BioGRID; 35536; 208.
DR IntAct; P07281; 5.
DR MINT; P07281; -.
DR STRING; 4932.YNL302C; -.
DR CarbonylDB; P07281; -.
DR iPTMnet; P07281; -.
DR MaxQB; P07281; -.
DR PaxDb; P07281; -.
DR TopDownProteomics; P07281; -.
DR EnsemblFungi; YNL302C_mRNA; YNL302C; YNL302C.
DR GeneID; 855414; -.
DR KEGG; sce:YNL302C; -.
DR SGD; S000005246; RPS19B.
DR VEuPathDB; FungiDB:YNL302C; -.
DR eggNOG; KOG3411; Eukaryota.
DR GeneTree; ENSGT00940000176238; -.
DR HOGENOM; CLU_108559_3_0_1; -.
DR InParanoid; P07281; -.
DR OMA; MTAPRNK; -.
DR BioCyc; YEAST:G3O-33290-MON; -.
DR Reactome; R-SCE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-SCE-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-SCE-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-SCE-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-SCE-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-SCE-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-SCE-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:P07281; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P07281; protein.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR GO; GO:0002181; P:cytoplasmic translation; IC:SGD.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD.
DR GO; GO:0006407; P:rRNA export from nucleus; IMP:SGD.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001266; Ribosomal_S19e.
DR InterPro; IPR018277; Ribosomal_S19e_CS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11710; PTHR11710; 1.
DR Pfam; PF01090; Ribosomal_S19e; 1.
DR SMART; SM01413; Ribosomal_S19e; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00628; RIBOSOMAL_S19E; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; Ribosome biogenesis.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:10601260,
FT ECO:0000269|PubMed:6814480"
FT CHAIN 2..144
FT /note="40S ribosomal protein S19-B"
FT /id="PRO_0000153836"
SQ SEQUENCE 144 AA; 15891 MW; A36AA94B855768CB CRC64;
MAGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS
VARHIYMRKQ VGVGKLNKLY GGAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG
GRRISENGQR DLDRIAAQTL EEDE
