B3GT2_ARATH
ID B3GT2_ARATH Reviewed; 407 AA.
AC A8MRC7; O23050;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable beta-1,3-galactosyltransferase 2;
DE EC=2.4.1.-;
GN Name=B3GALT2; OrderedLocusNames=At1g05170; ORFNames=YUP8H12.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18548197; DOI=10.1007/s11103-008-9351-3;
RA Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J.,
RA Bacic A.;
RT "Identification of a novel group of putative Arabidopsis thaliana beta-
RT (1,3)-galactosyltransferases.";
RL Plant Mol. Biol. 68:43-59(2008).
CC -!- FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from
CC UDP-galactose to substrates with a terminal glycosyl residue.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A8MRC7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A8MRC7-2; Sequence=VSP_036143;
CC -!- SIMILARITY: Belongs to the glycosyltransferase 31 family.
CC {ECO:0000305}.
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DR EMBL; AC000098; AAB71461.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27798.1; -; Genomic_DNA.
DR EMBL; AF389287; AAK63859.1; -; mRNA.
DR EMBL; AY113007; AAM47315.1; -; mRNA.
DR PIR; A86186; A86186.
DR RefSeq; NP_001077462.1; NM_001083993.2. [A8MRC7-1]
DR AlphaFoldDB; A8MRC7; -.
DR SMR; A8MRC7; -.
DR CAZy; GT31; Glycosyltransferase Family 31.
DR PaxDb; A8MRC7; -.
DR PRIDE; A8MRC7; -.
DR ProteomicsDB; 240957; -. [A8MRC7-1]
DR EnsemblPlants; AT1G05170.2; AT1G05170.2; AT1G05170. [A8MRC7-1]
DR GeneID; 839292; -.
DR Gramene; AT1G05170.2; AT1G05170.2; AT1G05170. [A8MRC7-1]
DR KEGG; ath:AT1G05170; -.
DR Araport; AT1G05170; -.
DR TAIR; locus:2207225; AT1G05170.
DR eggNOG; KOG2288; Eukaryota.
DR InParanoid; A8MRC7; -.
DR OMA; GRTTAME; -.
DR PhylomeDB; A8MRC7; -.
DR UniPathway; UPA00378; -.
DR PRO; PR:A8MRC7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A8MRC7; baseline and differential.
DR Genevisible; A8MRC7; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008378; F:galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR InterPro; IPR025298; DUF4094.
DR InterPro; IPR002659; Glyco_trans_31.
DR PANTHER; PTHR11214; PTHR11214; 1.
DR Pfam; PF13334; DUF4094; 1.
DR Pfam; PF01762; Galactosyl_T; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycosyltransferase; Golgi apparatus; Manganese;
KW Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..407
FT /note="Probable beta-1,3-galactosyltransferase 2"
FT /id="PRO_0000359412"
FT TRANSMEM 13..35
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT VAR_SEQ 70..72
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_036143"
SQ SEQUENCE 407 AA; 46162 MW; F58ECFF90B2D60E5 CRC64;
MSAKIKGEYS SRSFVSRKWT ILLCLGSFCV GMFFTNRMWN IPESKGMSHP SVTEAERLKL
VSEGCNPKAL YQKEVKRDPQ ALFGEVANTH IALQTLDKTI SSLEMELAAA RSVQESLQNG
APLSDDMGKK QPQEQRRFLM VVGINTAFSS RKRRDSIRAT WMPQGEKRKR LEEEKGIIIR
FVIGHSATTG GILDRAIEAE DRKHGDFLRL DHVEGYLELS GKTKTYFSTA FSMWDADFYV
KVDDDVHVNI ATLGETLVRH RKKPRVYIGC MKSGPVLSQK GVRYHEPEYW KFGENGNKYF
RHATGQLYAI SRDLASYISI NQHVLHKYAN EDVSLGAWFI GIDVKHIDDR RLCCGTPPDC
EWKAQAGNIC VASFDWSCSG ICRSADRIKE VHRRCGEGEK ALWSATF
