B3GT4_ARATH
ID B3GT4_ARATH Reviewed; 407 AA.
AC Q8LEJ9; Q3E9W1; Q9SZ33;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable beta-1,3-galactosyltransferase 4;
DE EC=2.4.1.-;
GN Name=B3GALT4; OrderedLocusNames=At4g26940; ORFNames=F10M23.280;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18548197; DOI=10.1007/s11103-008-9351-3;
RA Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J.,
RA Bacic A.;
RT "Identification of a novel group of putative Arabidopsis thaliana beta-
RT (1,3)-galactosyltransferases.";
RL Plant Mol. Biol. 68:43-59(2008).
CC -!- FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from
CC UDP-galactose to substrates with a terminal glycosyl residue.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LEJ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LEJ9-2; Sequence=VSP_036144, VSP_036145;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 31 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36540.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79549.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035440; CAB36540.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161566; CAB79549.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85272.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85273.1; -; Genomic_DNA.
DR EMBL; AY085383; AAM62612.1; -; mRNA.
DR PIR; T04817; T04817.
DR RefSeq; NP_567762.1; NM_118828.4. [Q8LEJ9-1]
DR RefSeq; NP_849454.1; NM_179123.3. [Q8LEJ9-2]
DR AlphaFoldDB; Q8LEJ9; -.
DR SMR; Q8LEJ9; -.
DR BioGRID; 14088; 1.
DR IntAct; Q8LEJ9; 1.
DR CAZy; GT31; Glycosyltransferase Family 31.
DR PaxDb; Q8LEJ9; -.
DR PRIDE; Q8LEJ9; -.
DR ProteomicsDB; 241115; -. [Q8LEJ9-1]
DR EnsemblPlants; AT4G26940.1; AT4G26940.1; AT4G26940. [Q8LEJ9-1]
DR EnsemblPlants; AT4G26940.2; AT4G26940.2; AT4G26940. [Q8LEJ9-2]
DR GeneID; 828801; -.
DR Gramene; AT4G26940.1; AT4G26940.1; AT4G26940. [Q8LEJ9-1]
DR Gramene; AT4G26940.2; AT4G26940.2; AT4G26940. [Q8LEJ9-2]
DR KEGG; ath:AT4G26940; -.
DR Araport; AT4G26940; -.
DR TAIR; locus:2116452; AT4G26940.
DR eggNOG; KOG2288; Eukaryota.
DR HOGENOM; CLU_040730_3_0_1; -.
DR InParanoid; Q8LEJ9; -.
DR OMA; EVTKTHH; -.
DR PhylomeDB; Q8LEJ9; -.
DR UniPathway; UPA00378; -.
DR PRO; PR:Q8LEJ9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LEJ9; baseline and differential.
DR Genevisible; Q8LEJ9; AT.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008378; F:galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR InterPro; IPR025298; DUF4094.
DR InterPro; IPR002659; Glyco_trans_31.
DR PANTHER; PTHR11214; PTHR11214; 1.
DR Pfam; PF13334; DUF4094; 1.
DR Pfam; PF01762; Galactosyl_T; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycosyltransferase; Golgi apparatus; Manganese;
KW Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..407
FT /note="Probable beta-1,3-galactosyltransferase 4"
FT /id="PRO_0000359414"
FT TRANSMEM 23..39
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT VAR_SEQ 282..306
FT /note="GVRYHEPEYWKFGEEGNKYFRHATG -> SALCNIQGVGVLHIDKPKRTSQI
FT CE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036144"
FT VAR_SEQ 307..407
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036145"
SQ SEQUENCE 407 AA; 46020 MW; 756C0F8866E1C952 CRC64;
MSLKHHHRGL ELSASKSFVS KKWTLFLCIG FFCAGILFSD RMWPEPESNV VSRDTVASDE
RLRLESEDCD SSKKGLKRES KDILGDVYKS PDAIQTLDKT ISKLETELAD ARAAQESIMN
GSPVSDDFKL PETVTKRKYL MVVGVNTAFS SRKRRDSVRA TWMPPGEERK KLEEEKGIVM
RFVIGHSSTP GGILDRAIQA EESKHGDFLR LDHVEGYLEL SAKTKTYFTT AFAMWDADFY
VKVDDDVHVN IATLGAELAR YRMKPRVYIG CMKSGPVLAQ KGVRYHEPEY WKFGEEGNKY
FRHATGQLYA ISRELASYIS INQNVLHKYV NEDVSLGSWF LGLDVEHVDD RRLCCGTTDC
EWKAQAGNIC VASFDWSCSG ICRSADRMKD VHRRCGEGEK ALLAASF
