ID   B4GN2_MOUSE             Reviewed;         510 AA.
AC   Q09199;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Beta-1,4 N-acetylgalactosaminyltransferase 2;
DE            EC=2.4.1.-;
GN   Name=B4galnt2; Synonyms=Galgt2, Ggm3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7515051; DOI=10.1016/s0021-9258(17)36587-0;
RA   Smith P.L., Lowe J.B.;
RT   "Molecular cloning of a murine N-acetylgalactosamine transferase cDNA that
RT   determines expression of the T lymphocyte-specific CT oligosaccharide
RT   differentiation antigen.";
RL   J. Biol. Chem. 269:15162-15171(1994).
CC   -!- FUNCTION: Responsible for synthesis of murine T-lymphocyte CT antigen.
CC       Can transfer N-acetylgalactosamine moiety from UDP-GalNAc to the low
CC       molecular weight acceptor 3'-sialyl-N-acetyllactosamine, to form a non-
CC       reducing terminal tetrasaccharide Sda blood group structure.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L30104; AAA39802.1; -; mRNA.
DR   CCDS; CCDS25285.1; -.
DR   PIR; A53802; A53802.
DR   RefSeq; NP_032107.1; NM_008081.3.
DR   AlphaFoldDB; Q09199; -.
DR   SMR; Q09199; -.
DR   STRING; 10090.ENSMUSP00000037239; -.
DR   CAZy; GT12; Glycosyltransferase Family 12.
DR   GlyGen; Q09199; 1 site.
DR   iPTMnet; Q09199; -.
DR   PhosphoSitePlus; Q09199; -.
DR   MaxQB; Q09199; -.
DR   PaxDb; Q09199; -.
DR   PRIDE; Q09199; -.
DR   ProteomicsDB; 277154; -.
DR   Antibodypedia; 2589; 80 antibodies from 22 providers.
DR   DNASU; 14422; -.
DR   Ensembl; ENSMUST00000038343; ENSMUSP00000037239; ENSMUSG00000013418.
DR   GeneID; 14422; -.
DR   KEGG; mmu:14422; -.
DR   UCSC; uc007lax.1; mouse.
DR   CTD; 124872; -.
DR   MGI; MGI:1342058; B4galnt2.
DR   VEuPathDB; HostDB:ENSMUSG00000013418; -.
DR   eggNOG; ENOG502QTK7; Eukaryota.
DR   GeneTree; ENSGT00390000006679; -.
DR   HOGENOM; CLU_036051_1_0_1; -.
DR   InParanoid; Q09199; -.
DR   OMA; KNHLQCS; -.
DR   OrthoDB; 363090at2759; -.
DR   PhylomeDB; Q09199; -.
DR   TreeFam; TF332297; -.
DR   Reactome; R-MMU-446203; Asparagine N-linked glycosylation.
DR   Reactome; R-MMU-9037629; Lewis blood group biosynthesis.
DR   UniPathway; UPA00378; -.
DR   BioGRID-ORCS; 14422; 3 hits in 74 CRISPR screens.
DR   PRO; PR:Q09199; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; Q09199; protein.
DR   Bgee; ENSMUSG00000013418; Expressed in duodenum and 54 other tissues.
DR   ExpressionAtlas; Q09199; baseline and differential.
DR   Genevisible; Q09199; MM.
DR   GO; GO:0030173; C:integral component of Golgi membrane; IEA:InterPro.
DR   GO; GO:0008376; F:acetylgalactosaminyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR   GO; GO:0022408; P:negative regulation of cell-cell adhesion; ISS:UniProtKB.
DR   GO; GO:0006486; P:protein glycosylation; IMP:MGI.
DR   GO; GO:0019276; P:UDP-N-acetylgalactosamine metabolic process; ISS:UniProtKB.
DR   GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IMP:MGI.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR011143; GM2_synthase.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   PIRSF; PIRSF000474; GM2_GD2_synthase; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..510
FT                   /note="Beta-1,4 N-acetylgalactosaminyltransferase 2"
FT                   /id="PRO_0000059104"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..32
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..510
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        390
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   510 AA;  58317 MW;  013783C80ADEC3CB CRC64;
     MTSSVSFASF RFPWLLKTFV LMVGLATVAF MVRKVSLTTD FSTFKPKFPE PARVDPVLKL
     LPEEHLRKLF TYSDIWLFPK NQCDCNSGKL RMKYKFQDAY NQKDLPAVNA RRQAEFEHFQ
     RREGLPRPPP LLAPPNLPFG YPVHGVEVMP LHTILIPGLQ YEGPDAPVYE VILKASLGTL
     NTLADVPDDE VQGRGQRQLT ISTRHRKVLN FILQHVTYTS TEYYLHKVDT VSMEYESSVA
     KFPVTIKQQT VPKLYDPGPE RKIRNLVTIA TKTFLRPHKL KILLQSIRKY YPDITVIVAD
     DSKEPLEIND DYVEYYTMPF GKGWFAGRNL AISQVTTKYV LWVDDDFLFS DKTKIEVLVD
     VLEKTELDVV GGSVQGNTYQ FRLLYEQTKN GSCLHQRWGS FQALDGFPGC TLTSGVVNFF
     LAHTEQLRRV GFDPILQRVA HGEFFIDGLG RLLVGSCPGV IINHQVRTPP KDPKLAALEK
     TYDKYRANTN SVIQFKVALQ YFKNHLYCST