RS20_BACSU
ID RS20_BACSU Reviewed; 88 AA.
AC P21477;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 4.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=30S ribosomal protein S20;
DE Short=BS20;
GN Name=rpsT; Synonyms=yqeO; OrderedLocusNames=BSU25550;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 18.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP PROTEIN SEQUENCE OF 2-31.
RX PubMed=6806564; DOI=10.1007/bf00330792;
RA Higo K., Otaka E., Osawa S.;
RT "Purification and characterization of 30S ribosomal proteins from Bacillus
RT subtilis: correlation to Escherichia coli 30S proteins.";
RL Mol. Gen. Genet. 185:239-244(1982).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
RC STRAIN=168;
RX PubMed=1840582; DOI=10.1128/jb.173.1.291-300.1991;
RA Sussman M.D., Setlow P.;
RT "Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr
RT gene, which codes for the protease that initiates degradation of small,
RT acid-soluble proteins during spore germination.";
RL J. Bacteriol. 173:291-300(1991).
RN [6] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-88 WITH AND WITHOUT
RP VIRGINIAMYCIN M, AND SUBUNIT.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: Binds directly to 16S ribosomal RNA.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit.
CC {ECO:0000269|PubMed:30126986}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bS20 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D84432; BAA12456.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14497.2; -; Genomic_DNA.
DR EMBL; M55263; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; C69701; C69701.
DR RefSeq; NP_390433.2; NC_000964.3.
DR RefSeq; WP_003229989.1; NZ_JNCM01000036.1.
DR PDB; 3J9W; EM; 3.90 A; AT=1-88.
DR PDB; 5NJT; EM; 3.80 A; T=3-88.
DR PDB; 6HA1; EM; 3.10 A; t=1-88.
DR PDB; 6HA8; EM; 3.50 A; t=1-88.
DR PDB; 6HTQ; EM; 4.50 A; t=4-86.
DR PDB; 7O5B; EM; 3.33 A; T=1-88.
DR PDB; 7QV1; EM; 3.50 A; t=1-88.
DR PDB; 7QV2; EM; 3.50 A; t=1-88.
DR PDB; 7QV3; EM; 5.14 A; t=1-88.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P21477; -.
DR SMR; P21477; -.
DR STRING; 224308.BSU25550; -.
DR jPOST; P21477; -.
DR PaxDb; P21477; -.
DR PRIDE; P21477; -.
DR EnsemblBacteria; CAB14497; CAB14497; BSU_25550.
DR GeneID; 937837; -.
DR KEGG; bsu:BSU25550; -.
DR PATRIC; fig|224308.179.peg.2776; -.
DR eggNOG; COG0268; Bacteria.
DR InParanoid; P21477; -.
DR OMA; GVIHKNA; -.
DR PhylomeDB; P21477; -.
DR BioCyc; BSUB:BSU25550-MON; -.
DR PRO; PR:P21477; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0070181; F:small ribosomal subunit rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.58.110; -; 1.
DR HAMAP; MF_00500; Ribosomal_S20; 1.
DR InterPro; IPR002583; Ribosomal_S20.
DR InterPro; IPR036510; Ribosomal_S20_sf.
DR PANTHER; PTHR33398; PTHR33398; 1.
DR Pfam; PF01649; Ribosomal_S20p; 1.
DR SUPFAM; SSF46992; SSF46992; 1.
DR TIGRFAMs; TIGR00029; S20; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:6806564"
FT CHAIN 2..88
FT /note="30S ribosomal protein S20"
FT /id="PRO_0000167920"
FT CONFLICT 18
FT /note="R -> G (in Ref. 1; BAA12456)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 88 AA; 9599 MW; E21D2717ABBA32C8 CRC64;
MPNIKSAIKR TKTNNERRVH NATIKSAMRT AIKQVEASVA NNEADKAKTA LTEAAKRIDK
AVKTGLVHKN TAARYKSRLA KKVNGLSA
