B5_VACCT
ID B5_VACCT Reviewed; 317 AA.
AC Q9JF44;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Protein B5;
DE AltName: Full=Plaque-size/host range protein;
DE Flags: Precursor;
GN Name=PS/HR; ORFNames=TB5R;
OS Vaccinia virus (strain Tian Tan) (VACV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10253;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Jin Q., Hou Y.D., Cheng N.H., Yao E.M., Cheng S.X., Yang X.K., Jing D.Y.,
RA Yu W.H., Yuan J.S., Ma X.J.;
RT "Complete genomic sequence of vaccinia virus (Tian Tan strain).";
RL Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulation of plaque size and host range.
CC -!- SUBUNIT: Interacts with A33; this interaction is required for efficient
CC targeting of A33 and B5 into enveloped virions. Interacts with A34;
CC this interaction is required for the correct glycosylation, trafficking
CC and stability of A34 and B5 incorporation into extracellular enveloped
CC virions. Interacts with envelope phospholipase F13.
CC {ECO:0000250|UniProtKB:Q01227}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q01227};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q01227}.
CC Host Golgi apparatus, host trans-Golgi network
CC {ECO:0000250|UniProtKB:Q01227}. Note=B5 is found on enveloped virion
CC (EV) membranes. {ECO:0000250|UniProtKB:Q01227}.
CC -!- SIMILARITY: Belongs to the receptors of complement activation (RCA)
CC family. {ECO:0000305}.
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DR EMBL; AF095689; AAF34074.1; -; Genomic_DNA.
DR SMR; Q9JF44; -.
DR Proteomes; UP000163220; Genome.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0001848; F:complement binding; IEA:InterPro.
DR GO; GO:0045916; P:negative regulation of complement activation; IEA:InterPro.
DR CDD; cd00033; CCP; 3.
DR InterPro; IPR011176; CCP_VACV_C3/B5.
DR InterPro; IPR035976; Sushi/SCR/CCP_sf.
DR InterPro; IPR000436; Sushi_SCR_CCP_dom.
DR Pfam; PF00084; Sushi; 3.
DR PIRSF; PIRSF002486; CIP_VAC_C3L; 1.
DR SMART; SM00032; CCP; 4.
DR SUPFAM; SSF57535; SSF57535; 3.
DR PROSITE; PS50923; SUSHI; 3.
PE 3: Inferred from homology;
KW Disulfide bond; Host Golgi apparatus; Lipoprotein; Membrane; Palmitate;
KW Repeat; Signal; Sushi; Transmembrane; Transmembrane helix; Virion.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..317
FT /note="Protein B5"
FT /id="PRO_0000006019"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 19..74
FT /note="Sushi 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 75..126
FT /note="Sushi 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 127..184
FT /note="Sushi 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 185..239
FT /note="Sushi 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT LIPID 301
FT /note="S-palmitoyl cysteine; by host"
FT /evidence="ECO:0000250|UniProtKB:Q01227"
FT LIPID 303
FT /note="S-palmitoyl cysteine; by host"
FT /evidence="ECO:0000250|UniProtKB:Q01227"
FT DISULFID 21..61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 48..72
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 76..110
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 101..125
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 130..171
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 157..182
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 186..225
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 211..237
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
SQ SEQUENCE 317 AA; 35160 MW; 823E611362E44851 CRC64;
MKTISVVTLL CVLPAVVYST CTVPTMNNAK LTSTETSFND KQKVTFTCDQ GYHSSDPNAV
CETDKWKYEN PCKKMCTVSD YISELYNKPL YEVNSTMTLS CNGETKYFRC EEKNGNTSWN
DTVTCPNAEC QPLQLEHGSC QPVKEKYSFG EYMTINCDVG YEVIGASYIS CTANSWNVIP
SCQQKCDMPS LSNGLISGST FSIGGVIHLS CKSGFILTGS PSSTCIDGKW NPVLPICVRT
NEEFDPVDDG PDDETDLSKL SKDVVQYEQE IESLEATYHI IMVALTIMGV IFLISVIVLV
CSCDKNNDQY KFHKLLP