ID   BA17B_METAN             Reviewed;         367 AA.
AC   P0DUT8;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=Polyenoic acids biosynthesis gene cluster protein Ba17b {ECO:0000303|PubMed:27801295};
DE   AltName: Full=PKS17 biosynthesis gene cluster protein Ba17b {ECO:0000303|PubMed:33991663};
GN   Name=Ba17b {ECO:0000303|PubMed:33991663}; ORFNames=MANI_002819;
OS   Metarhizium anisopliae (Entomophthora anisopliae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=5530;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=25263348; DOI=10.1186/1471-2164-15-822;
RA   Staats C.C., Junges A., Guedes R.L., Thompson C.E., de Morais G.L.,
RA   Boldo J.T., de Almeida L.G., Andreis F.C., Gerber A.L., Sbaraini N.,
RA   da Paixao R.L., Broetto L., Landell M., Santi L., Beys-da-Silva W.O.,
RA   Silveira C.P., Serrano T.R., de Oliveira E.S., Kmetzsch L., Vainstein M.H.,
RA   de Vasconcelos A.T., Schrank A.;
RT   "Comparative genome analysis of entomopathogenic fungi reveals a complex
RT   set of secreted proteins.";
RL   BMC Genomics 15:822-822(2014).
RN   [2]
RP   IDENTIFICATION, AND INDUCTION.
RX   PubMed=27801295; DOI=10.1186/s12864-016-3067-6;
RA   Sbaraini N., Guedes R.L., Andreis F.C., Junges A., de Morais G.L.,
RA   Vainstein M.H., de Vasconcelos A.T., Schrank A.;
RT   "Secondary metabolite gene clusters in the entomopathogen fungus
RT   Metarhizium anisopliae: genome identification and patterns of expression in
RT   a cuticle infection model.";
RL   BMC Genomics 17:736-736(2016).
RN   [3]
RP   FUNCTION.
RX   PubMed=33991663; DOI=10.1016/j.fgb.2021.103568;
RA   Sbaraini N., Hu J., Roux I., Phan C.S., Motta H., Rezaee H., Schrank A.,
RA   Chooi Y.H., Christian Staats C.;
RT   "Polyketides produced by the entomopathogenic fungus Metarhizium anisopliae
RT   induce Candida albicans growth.";
RL   Fungal Genet. Biol. 152:103568-103568(2021).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       (2Z,4E,6E,10E)-9-hydroxydodeca-2,4,6,10-tetraenoic acid (BAA),
CC       (2E,4E,6E,10E)-9-hydroxydodeca-2,4,6,10-tetraenoic acid (BAB), and
CC       (2Z,4E,6E)-octa-2,4,6-trienedioic acid (PBA) (PubMed:33991663). The
CC       highly reducing polyketide synthase Ba17a is sufficent to produce PBA
CC       and BAA (PubMed:33991663). The still to be characterized protein Ba17b
CC       leads to an increased production of BAA as well as to the production of
CC       the new compound BAB (PubMed:33991663). BAA does not possess
CC       insecticidal activity against G.mellonella larvae, however, both BAA
CC       and BAB increase the growth of Candida albicans and BAA can mitigate
CC       the fungicidal effects of fluconazole over C.albicans, suggesting that
CC       generalist pathogens such as M.anisopliae, can potentially manipulate
CC       the yeast microbiota found in arthropods (and anywhere else) by the
CC       activity of compounds as BAA and BAB (PubMed:33991663).
CC       {ECO:0000269|PubMed:33991663}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:33991663}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is induced under mimicked-infection conditions.
CC       {ECO:0000269|PubMed:27801295}.
CC   -!- SIMILARITY: Belongs to the SAT4 family. {ECO:0000305}.
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DR   EMBL; JNNZ01000213; KFG78607.1; -; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..367
FT                   /note="Polyenoic acids biosynthesis gene cluster protein
FT                   Ba17b"
FT                   /id="PRO_0000453548"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        133
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        245
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   367 AA;  39430 MW;  045EE8314D564D01 CRC64;
     MASEMNASPE YTGYRLEVFI AVFTPLTIIA VALRFYARSL TSKKIDSGDW LIIAALVGQI
     VAGGIAIGAV KQAGVGHHAA YLAETNPETL VAFFKYLVAM STWYATTEGL AKLAVCILYK
     RLFPQRGIHM VINTTMLVLV GASVGGGLAD LFGCTPFSAH WGTAEEQAAH CIDTEALFVW
     GSFPNIVTDV VLLVLPMPIV WGLHASVRLR LVLVLTFLFG SIFGELIGGD SGLITSVLRF
     IAFYNKSSFI DPTFHAVELI IWTVCEPGVY LIAACLLVYR PLLEKIGIPL VGGVSSRGGN
     RQEPTELAFQ KPSRPRNGAV IKSIGSGSIS ESGFEYIGDD DQRPLRRQGG ITATTNVEVT
     WAAGSAV