BAB2_DROME
ID BAB2_DROME Reviewed; 1067 AA.
AC Q9W0K4; Q24001; Q7YU69; Q9U1H3;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Protein bric-a-brac 2;
GN Name=bab2; Synonyms=BtbII; ORFNames=CG9102;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF47442.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Embryo, and Ovary;
RX PubMed=11973274; DOI=10.1242/dev.129.10.2419;
RA Couderc J.-L.G., Godt D., Zollman S., Chen J., Li M., Tiong S.,
RA Cramton S.E., Sahut-Barnola I., Laski F.A.;
RT "The bric a brac locus consists of two paralogous genes encoding BTB/POZ
RT domain proteins and acts as a homeotic and morphogenetic regulator of
RT imaginal development in Drosophila.";
RL Development 129:2419-2433(2002).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 196-310.
RX PubMed=7938017; DOI=10.1073/pnas.91.22.10717;
RA Zollman S., Godt D., Prive G.G., Couderc J.-L., Laski F.A.;
RT "The BTB domain, found primarily in zinc finger proteins, defines an
RT evolutionarily conserved family that includes several developmentally
RT regulated genes in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:10717-10721(1994).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-55; SER-56; SER-87; SER-147;
RP SER-377 AND THR-384, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Probably acts as a transcriptional regulator. Required for
CC the specification of the tarsal segment. Also involved in antenna
CC development. {ECO:0000269|PubMed:11973274,
CC ECO:0000303|PubMed:11973274}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00320,
CC ECO:0000269|PubMed:11973274}.
CC -!- TISSUE SPECIFICITY: Leg imaginal disk at the central region of the
CC tarsus and in eye antenna disk at the basal cylinder.
CC {ECO:0000269|PubMed:11973274}.
CC -!- MISCELLANEOUS: 'Bric-a-brac' means 'jumble' in French (referring to the
CC mutant ovary phenotype).
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DR EMBL; AJ252173; CAB64388.1; -; mRNA.
DR EMBL; AE014296; AAF47442.2; -; Genomic_DNA.
DR EMBL; BT009963; AAQ22432.1; -; mRNA.
DR EMBL; U14399; AAA50834.1; -; Genomic_DNA.
DR RefSeq; NP_523879.2; NM_079155.3.
DR AlphaFoldDB; Q9W0K4; -.
DR SMR; Q9W0K4; -.
DR BioGRID; 68922; 7.
DR DIP; DIP-20101N; -.
DR IntAct; Q9W0K4; 2.
DR STRING; 7227.FBpp0072535; -.
DR iPTMnet; Q9W0K4; -.
DR PaxDb; Q9W0K4; -.
DR EnsemblMetazoa; FBtr0072639; FBpp0072535; FBgn0025525.
DR GeneID; 44254; -.
DR KEGG; dme:Dmel_CG9102; -.
DR CTD; 44254; -.
DR FlyBase; FBgn0025525; bab2.
DR VEuPathDB; VectorBase:FBgn0025525; -.
DR eggNOG; ENOG502RYZ4; Eukaryota.
DR GeneTree; ENSGT00940000173981; -.
DR HOGENOM; CLU_009130_0_0_1; -.
DR InParanoid; Q9W0K4; -.
DR OrthoDB; 686400at2759; -.
DR PhylomeDB; Q9W0K4; -.
DR BioGRID-ORCS; 44254; 0 hits in 1 CRISPR screen.
DR ChiTaRS; bab2; fly.
DR GenomeRNAi; 44254; -.
DR PRO; PR:Q9W0K4; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0025525; Expressed in crop (Drosophila) and 47 other tissues.
DR ExpressionAtlas; Q9W0K4; baseline and differential.
DR Genevisible; Q9W0K4; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:FlyBase.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IDA:FlyBase.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IEP:UniProtKB.
DR GO; GO:0061040; P:female gonad morphogenesis; IMP:FlyBase.
DR GO; GO:0007478; P:leg disc morphogenesis; IEP:UniProtKB.
DR GO; GO:2000736; P:regulation of stem cell differentiation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:UniProtKB.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR007889; HTH_Psq.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF05225; HTH_psq; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS50960; HTH_PSQ; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1067
FT /note="Protein bric-a-brac 2"
FT /id="PRO_0000064792"
FT DOMAIN 223..288
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037,
FT ECO:0000305"
FT DOMAIN 635..687
FT /note="HTH psq-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00320"
FT DNA_BIND 645..690
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00320"
FT DNA_BIND 697..708
FT /note="A.T hook"
FT REGION 30..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 312..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 525..563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 796..829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 860..879
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 891..967
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..362
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 914..935
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 936..961
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 55
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 377
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 384
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 49
FT /note="E -> G (in Ref. 4; AAQ22432)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="D -> G (in Ref. 4; AAQ22432)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="R -> G (in Ref. 4; AAQ22432)"
FT /evidence="ECO:0000305"
FT CONFLICT 858
FT /note="A -> R (in Ref. 1; CAB64388)"
FT /evidence="ECO:0000305"
FT CONFLICT 911
FT /note="S -> F (in Ref. 4; AAQ22432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1067 AA; 114662 MW; 7DBFC7681D507FC0 CRC64;
MDMTKQIVDF EIKSELIGEI DQFEASDYTM APPEEPKMVE ESPQLGHLED QNRKYSPERE
VEPTLQDPSE VVDQMQKDTE SVGEVKSPEK DVETELVKSK ASPMNDQALT PPPRPLTSSE
VVGLRDPEHT ELRMCLEAKK SRSLPVSPQP QPNLKLAGSA LFEFGQRSSP VETKIKTNPE
TKPPRRKIVP PSGEGQQFCL RWNNYQSNLT NVFDELLQSE SFVDVTLSCE GHSIKAHKMV
LSACSPYFQA LFYDNPCQHP IIIMRDVSWS DLKALVEFMY KGEINVCQDQ INPLLKVAET
LKIRGLAEVS AGRGEGGASA LPMSAFDDED EEEELASATA ILQQDGDADP DEEMKAKRPR
LLPEGVLDLN QRQRKRSRDG SYATPSPSLQ GGESEISERG SSGTPGQSQS QPLAMTTSTI
VRNPFASPNP QTLEGRNSAM NAVANQRKSP APTATGHSNG NSGAAMHSPP GGVAVQSALP
PHMAAIVPPP PSAMHHHAQQ LAAQHQLAHS HAMASALAAA AAGAGAAGAG GAGSGSGSGA
SAPTGGTGVA GSGAGAAVGS HHDDMEIKPE IAEMIREEER AKMIESGGHG GWMGAAAAAT
GAASVAADSY QYQLQSMWQK CWNTNQQNLV QQLRFRERGP LKSWRPEAMA EAIFSVLKEG
LSLSQAARKF DIPYPTFVLY ANRVHNMLGP SLDGGADPRP KARGRPQRIL LGMWPEELIR
SVIKAVVFRD YREIKEDMSA HQYANGQGHG TYIGGGTTTN GYHSAAAAKL AAQNAALAPP
DAGSPLSSMT ETLRRQILSQ QQQHQQHHQQ QAHHQQQPSH HQQQSPHAQS MNMYKSPAYL
QRSEIEDQVS AAAAVAAAAA KHQQQQGERR GSENLPDLSA LGLMGLPGLN VMPSRGSGGG
SGGAAPNSAA SYARELSRER ERDRERERER ELSRQYGSQS RGSSSGSGSA KSLTASQRPG
AASPYSAAHY AKHQASAYNK RFLESLPAGI DLEAFANGLL QKSVNKSPRF EDFFPGPGQD
MSELFANPDA SAAAAAAAYA PPGAIRESPL MKIKLEQQHA TELPHED
