RS23_DROME
ID RS23_DROME Reviewed; 143 AA.
AC Q8T3U2; Q9V6Z0;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=40S ribosomal protein S23;
GN Name=RpS23; ORFNames=CG8415;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP HYDROXYLATION AT PRO-62.
RX PubMed=24550463; DOI=10.1073/pnas.1314485111;
RA Katz M.J., Acevedo J.M., Loenarz C., Galagovsky D., Liu-Yi P.,
RA Perez-Pepe M., Thalhammer A., Sekirnik R., Ge W., Melani M., Thomas M.G.,
RA Simonetta S., Boccaccio G.L., Schofield C.J., Cockman M.E., Ratcliffe P.J.,
RA Wappner P.;
RT "Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA
RT translation, cell homeostasis, and organ growth.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:4025-4030(2014).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (6.0 ANGSTROMS) OF THE 80S RIBOSOME,
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SUBUNIT: Component of the 40S small ribosomal subunit.
CC {ECO:0000269|PubMed:23636399}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P62266}. Cytoplasm
CC {ECO:0000305|PubMed:23636399}. Rough endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q6SA96}. Note=Detected on cytosolic polysomes
CC (By similarity). Detected in ribosomes that are associated with the
CC rough endoplasmic reticulum (By similarity).
CC {ECO:0000250|UniProtKB:P62266, ECO:0000250|UniProtKB:Q6SA96}.
CC -!- PTM: Hydroxylation at Pro-62 affects translation termination
CC efficiency. {ECO:0000305|PubMed:24550463}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAF58277.2; -; Genomic_DNA.
DR EMBL; AY089523; AAL90261.1; -; mRNA.
DR RefSeq; NP_001286410.1; NM_001299481.1.
DR RefSeq; NP_610939.2; NM_137095.3.
DR PDB; 4V6W; EM; 6.00 A; AX=1-143.
DR PDB; 6XU6; EM; 3.50 A; AX=1-143.
DR PDB; 6XU7; EM; 4.90 A; AX=1-143.
DR PDB; 6XU8; EM; 3.00 A; AX=1-143.
DR PDBsum; 4V6W; -.
DR PDBsum; 6XU6; -.
DR PDBsum; 6XU7; -.
DR PDBsum; 6XU8; -.
DR AlphaFoldDB; Q8T3U2; -.
DR SMR; Q8T3U2; -.
DR BioGRID; 62326; 111.
DR IntAct; Q8T3U2; 8.
DR MINT; Q8T3U2; -.
DR STRING; 7227.FBpp0086701; -.
DR PaxDb; Q8T3U2; -.
DR PRIDE; Q8T3U2; -.
DR DNASU; 36576; -.
DR EnsemblMetazoa; FBtr0087575; FBpp0086701; FBgn0033912.
DR EnsemblMetazoa; FBtr0339970; FBpp0308991; FBgn0033912.
DR GeneID; 36576; -.
DR KEGG; dme:Dmel_CG8415; -.
DR CTD; 6228; -.
DR FlyBase; FBgn0033912; RpS23.
DR VEuPathDB; VectorBase:FBgn0033912; -.
DR eggNOG; KOG1749; Eukaryota.
DR GeneTree; ENSGT00550000074784; -.
DR HOGENOM; CLU_115574_0_1_1; -.
DR InParanoid; Q8T3U2; -.
DR OMA; KFRWSQR; -.
DR OrthoDB; 1402984at2759; -.
DR PhylomeDB; Q8T3U2; -.
DR Reactome; R-DME-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-DME-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-DME-72649; Translation initiation complex formation.
DR Reactome; R-DME-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-DME-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-DME-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-DME-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-DME-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR SignaLink; Q8T3U2; -.
DR BioGRID-ORCS; 36576; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 36576; -.
DR PRO; PR:Q8T3U2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033912; Expressed in seminal fluid secreting gland and 13 other tissues.
DR ExpressionAtlas; Q8T3U2; baseline and differential.
DR Genevisible; Q8T3U2; DM.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0022626; C:cytosolic ribosome; IDA:FlyBase.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0042788; C:polysomal ribosome; ISS:UniProtKB.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0005791; C:rough endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:FlyBase.
DR GO; GO:0002181; P:cytoplasmic translation; ISS:UniProtKB.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03367; Ribosomal_S23; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005680; Ribosomal_S23_euk/arc.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00982; uS12_E_A; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Endoplasmic reticulum; Hydroxylation;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..143
FT /note="40S ribosomal protein S23"
FT /id="PRO_0000146465"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 62
FT /note="3-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:24550463"
SQ SEQUENCE 143 AA; 16017 MW; B1B700EC902F2C47 CRC64;
MGKPRGLRTA RKHVNHRRDQ RWADKDYKKA HLGTRWKANP FGGASHAKGI VLEKVGVEAK
QPNSAIRKCV RVQLIKNGKK ITAFVPRDGS LNYIEENDEV LVAGFGRKGH AVGDIPGVRF
KVVKVANVSL LALYKEKKER PRS
