RS27A_CHICK
ID RS27A_CHICK Reviewed; 156 AA.
AC P79781; P02248; P02249; P02250; P62973; Q29120; Q91887; Q91888;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 3.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Ubiquitin-40S ribosomal protein S27a;
DE AltName: Full=Ubiquitin carboxyl extension protein 80;
DE Contains:
DE RecName: Full=Ubiquitin;
DE Contains:
DE RecName: Full=40S ribosomal protein S27a;
DE Flags: Precursor;
GN Name=RPS27A; Synonyms=UBA80;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Hubbard White Mountain; TISSUE=Kidney, and Testis;
RX PubMed=9305777; DOI=10.1016/s0378-1119(97)00189-3;
RA Mezquita J., Pau M., Mezquita C.;
RT "Characterization and expression of two chicken cDNAs encoding ubiquitin
RT fused to ribosomal proteins of 52 and 80 amino acids.";
RL Gene 195:313-319(1997).
CC -!- FUNCTION: Ubiquitin Exists either covalently attached to another
CC protein, or free (unanchored). When covalently bound, it is conjugated
CC to target proteins via an isopeptide bond either as a monomer
CC (monoubiquitin), a polymer linked via different Lys residues of the
CC ubiquitin (polyubiquitin chains) or a linear polymer linked via the
CC initiator Met of the ubiquitin (linear polyubiquitin chains).
CC Polyubiquitin chains, when attached to a target protein, have different
CC functions depending on the Lys residue of the ubiquitin that is linked:
CC Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved
CC in ERAD (endoplasmic reticulum-associated degradation) and in cell-
CC cycle regulation; Lys-29-linked is involved in proteotoxic stress
CC response and cell cycle; Lys-33-linked is involved in kinase
CC modification; Lys-48-linked is involved in protein degradation via the
CC proteasome; Lys-63-linked is involved in endocytosis, DNA-damage
CC responses as well as in signaling processes leading to activation of
CC the transcription factor NF-kappa-B. Linear polymer chains formed via
CC attachment by the initiator Met lead to cell signaling. Ubiquitin is
CC usually conjugated to Lys residues of target proteins, however, in rare
CC cases, conjugation to Cys or Ser residues has been observed. When
CC polyubiquitin is free (unanchored-polyubiquitin), it also has distinct
CC roles, such as in activation of protein kinases, and in signaling (By
CC similarity). {ECO:0000250}.
CC -!- FUNCTION: Ribosomal protein S27a is a component of the 40S subunit of
CC the ribosome.
CC -!- SUBUNIT: Ribosomal protein S27a is part of the 40S ribosomal subunit.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Ubiquitin]: Cytoplasm {ECO:0000250}. Nucleus
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Ubiquitin is encoded by 4 different genes. Uba52 and
CC Rps27a genes code for a single copy of ubiquitin fused to the ribosomal
CC proteins L40 and S27a, respectively. UBB and UBC genes code for a
CC polyubiquitin precursor with exact head to tail repeats, the number of
CC repeats differ between species and strains.
CC -!- SIMILARITY: In the N-terminal section; belongs to the ubiquitin family.
CC {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the eukaryotic
CC ribosomal protein eS31 family. {ECO:0000305}.
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DR EMBL; U67931; AAC60279.1; -; mRNA.
DR RefSeq; NP_001274134.1; NM_001287205.1.
DR RefSeq; NP_990284.1; NM_204953.1.
DR PDB; 6ZVK; EM; 3.49 A; Q3=83-151.
DR PDBsum; 6ZVK; -.
DR AlphaFoldDB; P79781; -.
DR SMR; P79781; -.
DR STRING; 9031.ENSGALP00000013174; -.
DR PaxDb; P79781; -.
DR Ensembl; ENSGALT00000059625; ENSGALP00000050085; ENSGALG00000036790.
DR GeneID; 395796; -.
DR KEGG; gga:395796; -.
DR CTD; 6233; -.
DR VEuPathDB; HostDB:geneid_395796; -.
DR eggNOG; KOG0004; Eukaryota.
DR GeneTree; ENSGT00910000144152; -.
DR HOGENOM; CLU_010412_2_0_1; -.
DR InParanoid; P79781; -.
DR OMA; FMAQHAN; -.
DR OrthoDB; 1536766at2759; -.
DR PhylomeDB; P79781; -.
DR Reactome; R-GGA-110312; Translesion synthesis by REV1.
DR Reactome; R-GGA-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR Reactome; R-GGA-110320; Translesion Synthesis by POLH.
DR Reactome; R-GGA-1169091; Activation of NF-kappaB in B cells.
DR Reactome; R-GGA-1227882; TRAF mediated activation of IRF.
DR Reactome; R-GGA-1227888; Negative Regulation of MDA5 signaling.
DR Reactome; R-GGA-1227892; TRAF6 mediated NF-kB activation.
DR Reactome; R-GGA-1234176; Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
DR Reactome; R-GGA-1253288; Downregulation of ERBB4 signaling.
DR Reactome; R-GGA-1295596; Spry regulation of FGF signaling.
DR Reactome; R-GGA-1358803; Downregulation of ERBB2:ERBB3 signaling.
DR Reactome; R-GGA-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR Reactome; R-GGA-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR Reactome; R-GGA-174113; SCF-beta-TrCP mediated degradation of Emi1.
DR Reactome; R-GGA-174154; APC/C:Cdc20 mediated degradation of Securin.
DR Reactome; R-GGA-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-GGA-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR Reactome; R-GGA-179409; APC-Cdc20 mediated degradation of Nek2A.
DR Reactome; R-GGA-182971; EGFR downregulation.
DR Reactome; R-GGA-187577; SCF(Skp2)-mediated degradation of p27/p21.
DR Reactome; R-GGA-195253; Degradation of beta-catenin by the destruction complex.
DR Reactome; R-GGA-201681; TCF dependent signaling in response to WNT.
DR Reactome; R-GGA-205043; NRIF signals cell death from the nucleus.
DR Reactome; R-GGA-209560; NF-kB is activated and signals survival.
DR Reactome; R-GGA-2122948; Activated NOTCH1 Transmits Signal to the Nucleus.
DR Reactome; R-GGA-2173788; Downregulation of TGF-beta receptor signaling.
DR Reactome; R-GGA-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR Reactome; R-GGA-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-GGA-2467813; Separation of Sister Chromatids.
DR Reactome; R-GGA-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR Reactome; R-GGA-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-GGA-2672351; Stimuli-sensing channels.
DR Reactome; R-GGA-3134975; Regulation of innate immune responses to cytosolic DNA.
DR Reactome; R-GGA-349425; Autodegradation of the E3 ubiquitin ligase COP1.
DR Reactome; R-GGA-351465; Fanconi Anemia Pathway in DNA repair.
DR Reactome; R-GGA-353299; RAD18 and ubiquitinated PCNA-mediated recruitment of translesion polymerases.
DR Reactome; R-GGA-353303; Nucleotide Excision Repair.
DR Reactome; R-GGA-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-GGA-382556; ABC-family proteins mediated transport.
DR Reactome; R-GGA-433822; NFkB and MAPK activation mediated by TRAF6.
DR Reactome; R-GGA-433871; TRAF6 mediated induction of proinflammatory cytokines.
DR Reactome; R-GGA-434001; TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
DR Reactome; R-GGA-434131; NFkB activation mediated by RIP1 complexed with activated TLR3.
DR Reactome; R-GGA-437980; Activated TAK1 mediates p38 MAP kinase phosphorylation.
DR Reactome; R-GGA-437986; Activated TAK1 mediates Jun kinases (JNK) phosphorylation and activation.
DR Reactome; R-GGA-450302; activated TAK1 mediates p38 MAPK activation.
DR Reactome; R-GGA-450321; JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
DR Reactome; R-GGA-450408; AUF1 (hnRNP D0) binds and destabilizes mRNA.
DR Reactome; R-GGA-4641257; Degradation of AXIN.
DR Reactome; R-GGA-4641258; Degradation of DVL.
DR Reactome; R-GGA-4641263; Regulation of FZD by ubiquitination.
DR Reactome; R-GGA-532668; N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
DR Reactome; R-GGA-5357905; Regulation of TNFR1 signaling.
DR Reactome; R-GGA-5357956; TNFR1-induced NFkappaB signaling pathway.
DR Reactome; R-GGA-5358346; Hedgehog ligand biogenesis.
DR Reactome; R-GGA-5632684; Hedgehog 'on' state.
DR Reactome; R-GGA-5654726; Negative regulation of FGFR1 signaling.
DR Reactome; R-GGA-5654727; Negative regulation of FGFR2 signaling.
DR Reactome; R-GGA-5654732; Negative regulation of FGFR3 signaling.
DR Reactome; R-GGA-5654733; Negative regulation of FGFR4 signaling.
DR Reactome; R-GGA-5655862; Translesion synthesis by POLK.
DR Reactome; R-GGA-5656121; Translesion synthesis by POLI.
DR Reactome; R-GGA-5656169; Termination of translesion DNA synthesis.
DR Reactome; R-GGA-5668541; TNFR2 non-canonical NF-kB pathway.
DR Reactome; R-GGA-5675221; Negative regulation of MAPK pathway.
DR Reactome; R-GGA-5675482; Regulation of necroptotic cell death.
DR Reactome; R-GGA-5684264; MAP3K8 (TPL2)-dependent MAPK1/3 activation.
DR Reactome; R-GGA-5685942; HDR through Homologous Recombination (HRR).
DR Reactome; R-GGA-5689603; UCH proteinases.
DR Reactome; R-GGA-5689877; Josephin domain DUBs.
DR Reactome; R-GGA-5689880; Ub-specific processing proteases.
DR Reactome; R-GGA-5689896; Ovarian tumor domain proteases.
DR Reactome; R-GGA-5689901; Metalloprotease DUBs.
DR Reactome; R-GGA-5693565; Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
DR Reactome; R-GGA-5696394; DNA Damage Recognition in GG-NER.
DR Reactome; R-GGA-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-GGA-5696397; Gap-filling DNA repair synthesis and ligation in GG-NER.
DR Reactome; R-GGA-5696400; Dual Incision in GG-NER.
DR Reactome; R-GGA-573298; NFkB and MAPK activation mediated by TRAF6 upon TLR7 or TLR21 stimulation.
DR Reactome; R-GGA-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-GGA-6782135; Dual incision in TC-NER.
DR Reactome; R-GGA-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-GGA-6783310; Fanconi Anemia Pathway.
DR Reactome; R-GGA-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-GGA-6804757; Regulation of TP53 Degradation.
DR Reactome; R-GGA-6804760; Regulation of TP53 Activity through Methylation.
DR Reactome; R-GGA-6807004; Negative regulation of MET activity.
DR Reactome; R-GGA-68867; Assembly of the pre-replicative complex.
DR Reactome; R-GGA-68949; Orc1 removal from chromatin.
DR Reactome; R-GGA-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR Reactome; R-GGA-69231; Cyclin D associated events in G1.
DR Reactome; R-GGA-69541; Stabilization of p53.
DR Reactome; R-GGA-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-GGA-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-GGA-8849469; PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1.
DR Reactome; R-GGA-8854050; FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
DR Reactome; R-GGA-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-GGA-8856828; Clathrin-mediated endocytosis.
DR Reactome; R-GGA-8863795; Downregulation of ERBB2 signaling.
DR Reactome; R-GGA-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-GGA-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-GGA-8939236; RUNX1 regulates transcription of genes involved in differentiation of HSCs.
DR Reactome; R-GGA-8939902; Regulation of RUNX2 expression and activity.
DR Reactome; R-GGA-8941858; Regulation of RUNX3 expression and activity.
DR Reactome; R-GGA-8948747; Regulation of PTEN localization.
DR Reactome; R-GGA-8948751; Regulation of PTEN stability and activity.
DR Reactome; R-GGA-8951664; Neddylation.
DR Reactome; R-GGA-901032; ER Quality Control Compartment (ERQC).
DR Reactome; R-GGA-9013507; NOTCH3 Activation and Transmission of Signal to the Nucleus.
DR Reactome; R-GGA-9033241; Peroxisomal protein import.
DR Reactome; R-GGA-912631; Regulation of signaling by CBL.
DR Reactome; R-GGA-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR Reactome; R-GGA-917937; Iron uptake and transport.
DR Reactome; R-GGA-936964; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
DR Reactome; R-GGA-937041; IKK complex recruitment mediated by RIP1.
DR Reactome; R-GGA-937042; IRAK2 mediated activation of TAK1 complex.
DR Reactome; R-GGA-937072; TRAF6-mediated induction of TAK1 complex within TLR4 complex.
DR Reactome; R-GGA-9645460; Alpha-protein kinase 1 signaling pathway.
DR Reactome; R-GGA-9646399; Aggrephagy.
DR Reactome; R-GGA-9664873; Pexophagy.
DR Reactome; R-GGA-9708530; Regulation of BACH1 activity.
DR Reactome; R-GGA-975163; IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation.
DR Reactome; R-GGA-9755511; KEAP1-NFE2L2 pathway.
DR Reactome; R-GGA-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-GGA-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR Reactome; R-GGA-9762114; GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2.
DR Reactome; R-GGA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:P79781; -.
DR Proteomes; UP000000539; Chromosome 3.
DR Bgee; ENSGALG00000036790; Expressed in granulocyte and 13 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0031386; F:protein tag; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0019941; P:modification-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 6.20.50.150; -; 1.
DR InterPro; IPR002906; Ribosomal_S27a.
DR InterPro; IPR011332; Ribosomal_zn-bd.
DR InterPro; IPR038582; S27a-like_sf.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR019954; Ubiquitin_CS.
DR InterPro; IPR019956; Ubiquitin_dom.
DR Pfam; PF01599; Ribosomal_S27; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR PRINTS; PR00348; UBIQUITIN.
DR SMART; SM01402; Ribosomal_S27; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR SUPFAM; SSF57829; SSF57829; 1.
DR PROSITE; PS00299; UBIQUITIN_1; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Isopeptide bond; Metal-binding; Nucleus;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation;
KW Zinc; Zinc-finger.
FT CHAIN 1..76
FT /note="Ubiquitin"
FT /id="PRO_0000396481"
FT CHAIN 77..156
FT /note="40S ribosomal protein S27a"
FT /id="PRO_0000137665"
FT DOMAIN 1..76
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT ZN_FING 121..144
FT /note="C4-type"
FT REGION 76..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 54
FT /note="Interacts with activating enzyme"
FT SITE 68
FT /note="Essential for function"
FT SITE 72
FT /note="Interacts with activating enzyme"
FT CROSSLNK 6
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 11
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 27
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 29
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 33
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 48
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 63
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62979"
FT CROSSLNK 76
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-? in acceptor proteins)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
SQ SEQUENCE 156 AA; 17979 MW; 60D0DC8DF3A904F7 CRC64;
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN
IQKESTLHLV LRLRGGAKKR KKKSYTTPKK NKHKRKKVKL AVLKYYKVDE NGKISRLRRE
CPSEECGAGV FMASHFDRHY CGKCCLTYCF NKPEDK
