BACE_BACSU
ID BACE_BACSU Reviewed; 394 AA.
AC P39642;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Putative bacilysin exporter BacE;
GN Name=bacE; Synonyms=ywfF; OrderedLocusNames=BSU37700; ORFNames=ipa-84d;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7934828; DOI=10.1111/j.1365-2958.1993.tb01963.x;
RA Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F.,
RA Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E.,
RA Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A.;
RT "Bacillus subtilis genome project: cloning and sequencing of the 97 kb
RT region from 325 degrees to 333 degrees.";
RL Mol. Microbiol. 10:371-384(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP REGULATION.
RC STRAIN=168 / 61884;
RX PubMed=12372825; DOI=10.1074/jbc.m208722200;
RA Inaoka T., Takahashi K., Ohnishi-Kameyama M., Yoshida M., Ochi K.;
RT "Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-
RT operatively regulate the production of an antibiotic bacilysin in Bacillus
RT subtilis.";
RL J. Biol. Chem. 278:2169-2176(2003).
RN [4]
RP FUNCTION IN BACILYSIN PRODUCTION, AND GENE NAME.
RX PubMed=15609023; DOI=10.1007/s00203-004-0743-8;
RA Steinborn G., Hajirezaei M.-R., Hofemeister J.;
RT "bac genes for recombinant bacilysin and anticapsin production in Bacillus
RT host strains.";
RL Arch. Microbiol. 183:71-79(2005).
CC -!- FUNCTION: Part of the bacilysin biosynthesis operon. May be involved in
CC self-resistance to bacilysin by permitting efflux of this antibiotic.
CC {ECO:0000269|PubMed:15609023}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: The compound ppGpp is essential for the transcription of
CC the bacABCDE operon and GTP regulates the transcription of both this
CC operon and ywfH via the CodY-mediated regulation system.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Drug:H(+)
CC antiporter-3 (DHA3) (TC 2.A.1.21) family. {ECO:0000305}.
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DR EMBL; X73124; CAA51640.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15797.1; -; Genomic_DNA.
DR PIR; S39739; S39739.
DR RefSeq; NP_391650.1; NC_000964.3.
DR RefSeq; WP_003244502.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P39642; -.
DR SMR; P39642; -.
DR STRING; 224308.BSU37700; -.
DR TCDB; 2.A.1.21.5; the major facilitator superfamily (mfs).
DR PaxDb; P39642; -.
DR PRIDE; P39642; -.
DR EnsemblBacteria; CAB15797; CAB15797; BSU_37700.
DR GeneID; 937161; -.
DR KEGG; bsu:BSU37700; -.
DR PATRIC; fig|224308.179.peg.4082; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; P39642; -.
DR OMA; IAMEYGE; -.
DR BioCyc; BSUB:BSU37700-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR022324; Bacilysin_exporter_BacE_put.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01988; EXPORTERBACE.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..394
FT /note="Putative bacilysin exporter BacE"
FT /id="PRO_0000084834"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 394 AA; 43417 MW; CFEEB079F0A56A98 CRC64;
MKQLKPNSKY LLYGQALSFM GDYCVLPALL ILSTYYHDYW VTSGVIVVRS IPMVFQPFLG
VLVDRLDRIK IMLWTDIIRG IIFLGLTFLP KGEYPLIFLA LLFITYGSGV FFNPARLAVM
SSLESDIKSI NTLFAKATTI SIIVGAAAGG LFLLGGSVEL AVAFNGVTYL VSAFFISRIK
LQFVPIQSEN IKEAFQSFKE GLKEIKTNSF VLNAMFTMIT MALLWGVVYS YFPIVSRFLG
DGEIGNFILT FCIGFGGFIG AALVSKWGFN NNRGLTYFTV LSIVSLALFL FTPIFAVSVI
AAILFFIAME YGEVLAKVKV QENAANQIQG RIFSVAEASI GLCISIGSMF INILSAPVIM
GLIVVIVCGL FLHTKLVNKS FLERDNKTEQ KGVF
