RS27_MOUSE
ID RS27_MOUSE Reviewed; 84 AA.
AC Q6ZWU9; Q3TLE3;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=40S ribosomal protein S27;
GN Name=Rps27 {ECO:0000312|MGI:MGI:1888676};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Head, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000312|EMBL:AAH55693.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH55693.1}, and FVB/N-3;
RC TISSUE=Brain {ECO:0000312|EMBL:AAH55693.1}, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, and
RC Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the small ribosomal subunit (By similarity).
CC Required for proper rRNA processing and maturation of 18S rRNAs (By
CC similarity). {ECO:0000250|UniProtKB:P42677}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC -!- SUBUNIT: Component of the small ribosomal subunit.
CC {ECO:0000250|UniProtKB:P42677}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eS27 family.
CC {ECO:0000305}.
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DR EMBL; AK014294; BAB29250.1; -; mRNA.
DR EMBL; AK088320; BAC40279.1; -; mRNA.
DR EMBL; AK166551; BAE38849.1; -; mRNA.
DR EMBL; BC048352; AAH48352.1; -; mRNA.
DR EMBL; BC055693; AAH55693.1; -; mRNA.
DR CCDS; CCDS50970.1; -.
DR RefSeq; NP_001177187.1; NM_001190258.1.
DR RefSeq; NP_081291.1; NM_027015.4.
DR PDB; 7CPU; EM; 2.82 A; Sb=1-84.
DR PDB; 7CPV; EM; 3.03 A; Sb=1-84.
DR PDBsum; 7CPU; -.
DR PDBsum; 7CPV; -.
DR AlphaFoldDB; Q6ZWU9; -.
DR SMR; Q6ZWU9; -.
DR BioGRID; 208248; 38.
DR BioGRID; 786971; 4.
DR IntAct; Q6ZWU9; 2.
DR MINT; Q6ZWU9; -.
DR STRING; 10090.ENSMUSP00000060523; -.
DR iPTMnet; Q6ZWU9; -.
DR PhosphoSitePlus; Q6ZWU9; -.
DR SwissPalm; Q6ZWU9; -.
DR EPD; Q6ZWU9; -.
DR jPOST; Q6ZWU9; -.
DR MaxQB; Q6ZWU9; -.
DR PaxDb; Q6ZWU9; -.
DR PeptideAtlas; Q6ZWU9; -.
DR PRIDE; Q6ZWU9; -.
DR ProteomicsDB; 260855; -.
DR TopDownProteomics; Q6ZWU9; -.
DR DNASU; 57294; -.
DR Ensembl; ENSMUST00000053150; ENSMUSP00000060523; ENSMUSG00000050621.
DR Ensembl; ENSMUST00000170122; ENSMUSP00000132102; ENSMUSG00000090733.
DR GeneID; 100043813; -.
DR GeneID; 57294; -.
DR KEGG; mmu:100043813; -.
DR KEGG; mmu:57294; -.
DR UCSC; uc008qbl.2; mouse.
DR CTD; 100043813; -.
DR CTD; 6232; -.
DR MGI; MGI:1888676; Rps27.
DR VEuPathDB; HostDB:ENSMUSG00000050621; -.
DR VEuPathDB; HostDB:ENSMUSG00000090733; -.
DR eggNOG; KOG1779; Eukaryota.
DR GeneTree; ENSGT00950000182891; -.
DR HOGENOM; CLU_130128_3_0_1; -.
DR InParanoid; Q6ZWU9; -.
DR OMA; VYSHANT; -.
DR OrthoDB; 949920at2759; -.
DR PhylomeDB; Q6ZWU9; -.
DR TreeFam; TF300265; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-MMU-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-72649; Translation initiation complex formation.
DR Reactome; R-MMU-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-MMU-72695; Formation of the ternary complex, and subsequently, the 43S complex.
DR Reactome; R-MMU-72702; Ribosomal scanning and start codon recognition.
DR Reactome; R-MMU-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR Reactome; R-MMU-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-MMU-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 100043813; 14 hits in 38 CRISPR screens.
DR BioGRID-ORCS; 57294; 28 hits in 52 CRISPR screens.
DR ChiTaRS; Rps27; mouse.
DR PRO; PR:Q6ZWU9; -.
DR Proteomes; UP000000589; Chromosome 3.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q6ZWU9; protein.
DR Bgee; ENSMUSG00000050621; Expressed in adrenal gland and 61 other tissues.
DR ExpressionAtlas; Q6ZWU9; baseline and differential.
DR Genevisible; Q6ZWU9; MM.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0022626; C:cytosolic ribosome; ISO:MGI.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; ISO:MGI.
DR GO; GO:0098982; C:GABA-ergic synapse; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0014069; C:postsynaptic density; ISO:MGI.
DR GO; GO:0098793; C:presynapse; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; ISO:MGI.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 2.20.25.100; -; 1.
DR HAMAP; MF_00371; Ribosomal_S27e; 1.
DR InterPro; IPR000592; Ribosomal_S27.
DR InterPro; IPR023407; Ribosomal_S27_Zn-bd_dom_sf.
DR InterPro; IPR011332; Ribosomal_zn-bd.
DR PANTHER; PTHR11594; PTHR11594; 1.
DR Pfam; PF01667; Ribosomal_S27e; 1.
DR SUPFAM; SSF57829; SSF57829; 1.
DR PROSITE; PS01168; RIBOSOMAL_S27E; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Phosphoprotein; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; Zinc; Zinc-finger.
FT CHAIN 1..84
FT /note="40S ribosomal protein S27"
FT /id="PRO_0000149052"
FT ZN_FING 38..60
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
SQ SEQUENCE 84 AA; 9461 MW; 242C4466AC8A8900 CRC64;
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS
TVLCQPTGGK ARLTEGCSFR RKQH
