ID   RS2_MAIZE               Reviewed;         370 AA.
AC   Q9S7B2;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 139.
DE   RecName: Full=Protein rough sheath 2;
DE   AltName: Full=Protein PHANTASTICA;
DE   AltName: Full=ZmPHAN;
GN   Name=RS2;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND FUNCTION.
RC   STRAIN=cv. B73;
RX   PubMed=10102816; DOI=10.1126/science.284.5411.151;
RA   Timmermans M.C.P., Hudson A., Becraft P.W., Nelson T.;
RT   "ROUGH SHEATH2: a Myb protein that represses knox homeobox genes in maize
RT   lateral organ primordia.";
RL   Science 284:151-153(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INTERACTION WITH AS2;
RP   WRKY1; RIK AND HIRA.
RX   PubMed=16243907; DOI=10.1105/tpc.105.035477;
RA   Phelps-Durr T.L., Thomas J., Vahab P., Timmermans M.C.P.;
RT   "Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1
RT   interact with HIRA, a predicted histone chaperone, to maintain knox gene
RT   silencing and determinacy during organogenesis.";
RL   Plant Cell 17:2886-2898(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10102817; DOI=10.1126/science.284.5411.154;
RA   Tsiantis M., Schneeberger R., Golz J.F., Freeling M., Langdale J.A.;
RT   "The maize rough sheath2 gene and leaf development programs in monocot and
RT   dicot plants.";
RL   Science 284:154-156(1999).
RN   [4]
RP   FUNCTION.
RX   PubMed=9655808; DOI=10.1242/dev.125.15.2857;
RA   Schneeberger R., Tsiantis M., Freeling M., Langdale J.A.;
RT   "The rough sheath2 gene negatively regulates homeobox gene expression
RT   during maize leaf development.";
RL   Development 125:2857-2865(1998).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=12750468; DOI=10.1073/pnas.1132113100;
RA   Theodoris G., Inada N., Freeling M.;
RT   "Conservation and molecular dissection of ROUGH SHEATH2 and ASYMMETRIC
RT   LEAVES1 function in leaf development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:6837-6842(2003).
CC   -!- FUNCTION: Transcription factor required for normal cell
CC       differentiation. Interacts directly with asymmetric leaves 2 (AS2) to
CC       repress the knox homeobox genes. {ECO:0000269|PubMed:10102816,
CC       ECO:0000269|PubMed:12750468, ECO:0000269|PubMed:9655808}.
CC   -!- SUBUNIT: Homodimer. Interacts with AS2, WRKY1, HIRA, a probable histone
CC       chaperone, and RIK, a predicted RNA binding protein.
CC       {ECO:0000269|PubMed:12750468, ECO:0000269|PubMed:16243907}.
CC   -!- INTERACTION:
CC       Q9S7B2; Q32SG3: LBD6; NbExp=2; IntAct=EBI-761350, EBI-761239;
CC       Q9S7B2; Q32SG4; NbExp=2; IntAct=EBI-761350, EBI-761253;
CC       Q9S7B2; Q32SG5; NbExp=2; IntAct=EBI-761350, EBI-761296;
CC       Q9S7B2; Q32SG6; NbExp=2; IntAct=EBI-761350, EBI-761318;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC       ECO:0000269|PubMed:12750468}. Note=Localized in discrete subnuclear
CC       bodies.
CC   -!- TISSUE SPECIFICITY: Expressed in lateral organ promordia.
CC       {ECO:0000269|PubMed:10102816, ECO:0000269|PubMed:16243907}.
CC   -!- DOMAIN: A C-terminal domain (245-347) is required for dimerization.
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DR   EMBL; AF126489; AAD25082.1; -; Genomic_DNA.
DR   EMBL; AF143447; AAD33898.1; -; mRNA.
DR   RefSeq; NP_001105509.1; NM_001112039.2.
DR   AlphaFoldDB; Q9S7B2; -.
DR   SMR; Q9S7B2; -.
DR   IntAct; Q9S7B2; 4.
DR   STRING; 4577.GRMZM2G403620_P01; -.
DR   PaxDb; Q9S7B2; -.
DR   EnsemblPlants; Zm00001eb029300_T001; Zm00001eb029300_P001; Zm00001eb029300.
DR   EnsemblPlants; Zm00001eb029300_T002; Zm00001eb029300_P002; Zm00001eb029300.
DR   GeneID; 542488; -.
DR   Gramene; Zm00001eb029300_T001; Zm00001eb029300_P001; Zm00001eb029300.
DR   Gramene; Zm00001eb029300_T002; Zm00001eb029300_P002; Zm00001eb029300.
DR   KEGG; zma:542488; -.
DR   MaizeGDB; 726059; -.
DR   eggNOG; KOG0048; Eukaryota.
DR   HOGENOM; CLU_864276_0_0_1; -.
DR   OMA; KSCAERW; -.
DR   OrthoDB; 959551at2759; -.
DR   Proteomes; UP000007305; Chromosome 1.
DR   ExpressionAtlas; Q9S7B2; baseline and differential.
DR   Genevisible; Q9S7B2; ZM.
DR   GO; GO:0000793; C:condensed chromosome; IEA:EnsemblPlants.
DR   GO; GO:0005730; C:nucleolus; IEA:EnsemblPlants.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblPlants.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IEA:EnsemblPlants.
DR   GO; GO:0008356; P:asymmetric cell division; IEA:EnsemblPlants.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:EnsemblPlants.
DR   GO; GO:0050832; P:defense response to fungus; IEA:EnsemblPlants.
DR   GO; GO:0010338; P:leaf formation; IEA:EnsemblPlants.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR   GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IEA:EnsemblPlants.
DR   GO; GO:0009946; P:proximal/distal axis specification; IEA:EnsemblPlants.
DR   GO; GO:0045088; P:regulation of innate immune response; IEA:EnsemblPlants.
DR   GO; GO:0009615; P:response to virus; IEA:EnsemblPlants.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00167; SANT; 2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR031055; RS2/Asn1.
DR   InterPro; IPR001005; SANT/Myb.
DR   PANTHER; PTHR47214:SF3; PTHR47214:SF3; 1.
DR   SMART; SM00717; SANT; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS51294; HTH_MYB; 2.
PE   1: Evidence at protein level;
KW   Coiled coil; DNA-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..370
FT                   /note="Protein rough sheath 2"
FT                   /id="PRO_0000299135"
FT   DOMAIN          1..53
FT                   /note="HTH myb-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DOMAIN          54..108
FT                   /note="HTH myb-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        27..53
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        81..104
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          107..129
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          276..340
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   370 AA;  41919 MW;  B46825CA38003EE4 CRC64;
     MKERQRWRPE EDAVLRAYVR QYGPREWHLV SQRMNVALDR DAKSCLERWK NYLRPGIKKG
     SLTEEEQRLV IRLQAKHGNK WKKIAAEVPG RTAKRLGKWW EVFKEKQQRE LRDSRRPPPE
     PSPDERGRYE WLLENFAEKL VGERPQQAAA APSPLLMAAP VLPPWLSSNA GPAAAAAAAV
     AHPPPRPPSP SVTLSLASAA VAPGPPAPAP WMPDRAAADA APYGFPSPSQ HGGAAPPGMA
     VVDGQALAEL AECCRELEEG RRAWAAHRRE AAWRLKRVEQ QLEMEREMRR REVWEEFEAK
     MRTMRLEQAA AAERVERDHR EKVAELRRDA QVKEEKMAEQ WAAKHARVAK FVEQMGGCSR
     SWSSATDMNC